Composition containing berberine and timosaponin and application of composition

A technology of timosaponin and composition, applied in the direction of medical preparations containing active ingredients, applications, drug combinations, etc., can solve the problem of large toxic and side effects, lack of significant drug efficacy, active ingredients and mechanism of action, and aggravation of cardiovascular complications And other issues

Inactive Publication Date: 2017-09-22
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It also includes chemical drugs. Although they have a strong hypoglycemic effect, they have large toxic and side effects on the liver and kidneys, and can easily lead to aggravation of cardiovascular complications. There are also some drugs, such as: glucagon peptides (Glucagon -like peptide 1, GLP-1), fatty acylated glucagon peptide (NN2211, Novo Nordisk), Exendin-4 ( Amylin) and polyethylene glycol-modified Exendin-4, etc., can effectively control blood sugar, reduce glycosylated hemoglobin HbA1c value and improve β-islet cell function after long-term administration, but because these molecules also act on central nervous receptors, so Symptoms of vomiting and dizziness after administration are di...

Method used

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  • Composition containing berberine and timosaponin and application of composition
  • Composition containing berberine and timosaponin and application of composition
  • Composition containing berberine and timosaponin and application of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0134] Preparation of Timosaponin BⅡ

[0135] Take 5KG of commercially available Chinese medicine Anemarrhena decoction pieces, heat and reflux extraction with 90% (v / v) ethanol aqueous solution, recover the ethanol under reduced pressure, add 20L of water to heat to dissolve, centrifuge, discard the precipitate, and pass the supernatant through AB- 8 Macroporous resin (5L) adsorption separation, the supernatant was passed through the macroporous adsorption resin, washed with 3 times the volume (BV) of water to remove impurities, and then 10% (v / v) of 3 times the volume (BV) Aqueous ethanol is used as eluent for eluting, discarding the eluent; then eluting with 35% ethanol, collecting 12 times the volume (BV) of the eluent, recovering the ethanol in the eluent under reduced pressure, and drying to obtain Crude timosaponin BⅡ. After the crude timosaponin BⅡ was dissolved in methanol, 100g of ODS was added to mix the sample, and the sample was loaded onto a 2L ODS column, and t...

Embodiment 2

[0141] Preparation of berberine hydrochloride

[0142] Take 2 kg of Cortex Phellodendri decoction pieces, add water and decoct three times, 2 hours each time, filter the decoction, combine the filtrate, pass the filtrate through the AB-8 macroporous adsorption resin column (3L), and wash it with 1 times the volume (BV) of water , then eluted with 1 times volume (BV) of 20% ethanol water, discarded the 20% ethanol water eluate, then eluted with 40% ethanol water, collected 4 times volume (BV) of 40% ethanol water eluate ), concentrated into a thick paste. Add water to the thick paste and heat to 90°C, filter while it is hot to remove insoluble matter, add concentrated hydrochloric acid while stirring until the pH is 1-2, and precipitate berberine hydrochloride, let it stand overnight, filter, and repeat the crystallization once more. Add water and wash until the filtrate is nearly neutral, dry and pulverize to obtain 30.3 g, the content of which is 97% as determined by HPLC. ...

Embodiment 3

[0144] Preparation of Zhimu Extract

[0145] Get 10kg of commercially available Chinese medicine Anemarrhena decoction pieces, heat and reflux extraction with 30% methanol aqueous solution, after the methanol is recovered from the extract, dilute with water, filter, and then separate the filtrate through the adsorption method of AB-8 macroporous adsorption resin (10L) to make The filtrate of the extract is passed through a macroporous adsorption resin column, washed with 1 volume volume (BV) of water to remove impurities, then eluted with 2 volume volume (BV) of 0.2% sodium hydroxide, washed with water until neutral, and then washed with 3 volume volumes (BV) of The ethanol aqueous solution of volume (BV) 10v / v% is eluted as eluent, discards this eluent; Then 40% ethanol is eluted, collects eluent 4 times of volume (BV), reclaims under reduced pressure washing The ethanol in the deliquification was dried to obtain 498g of total mother saponins. The above-mentioned 0.2% sodium...

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PUM

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Abstract

The invention provides a composition and application thereof, and particularly provides a composition for decreasing blood sugar. The composition contains a first active component with berberine being an effective component and a second active component with timosaponin being the effective component; moreover, the effective component ratio V1 (mg: mg) of the first active component to the second active component is (1.5-6): 1. The composition can fast and effectively decrease the blood sugar and the drug feeding dose of the composition can be remarkably lowered at the same time, and adverse reactions of drugs can be reduced and a safe window is expanded.

Description

technical field [0001] The invention relates to the field of food and medicine, more specifically to a composition containing berberine and timosaponin, and the application of the composition in lowering blood sugar. Background technique [0002] Diabetes Mellitus (DM) is a series of clinical syndromes caused by absolute or relative insufficiency of insulin in the body. The disease can also cause some complications during the development process, such as: hypoglycemia (Hypoglycemia), ketoacidosis (Ketoacidosis), nonketotic hyperosmolar coma (Nonketotic Hyperosmolar Coma), cardiovascular disease, chronic renal failure, retinal disease, neuropathy, and microvascular disease. [0003] There are three main categories of drugs commonly used in the treatment of diabetes. The first category is biological drugs, such as insulin (Insulin); chemical drugs, such as: sulfonylureas (Sulfonylurea), biguanides (Biguanide), glitazones (Glitazone) ) and Chinese patent medicines. Due to th...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P3/10A23L33/105A23L2/52A61K31/4375
CPCA23L2/52A23V2002/00A61K31/4375A61K31/7048A61K2300/00A23V2200/328A23V2250/21
Inventor 黄成钢李佳李志雄李静雅孙兆林潘国宇陈明苍田小亭崔仕超林云飞刘磊江磊丁健
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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