Mesenchymal stem cell factor composition as well as preparation method and application thereof

A technology of stem cells and hepatocyte growth factor, which is applied in the field of skin repair, can solve the problems of low extraction rate and inconvenient liquefaction, and achieve the effect of increasing skin elasticity and plumpness, stable product properties, and long-term biological activity

Active Publication Date: 2017-10-20
陕西德健众普生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, the extract of mesenchymal stem cells in the prior art has many disadvantages such as low extraction rate and inconvenient preservation in liquefaction,

Method used

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  • Mesenchymal stem cell factor composition as well as preparation method and application thereof
  • Mesenchymal stem cell factor composition as well as preparation method and application thereof
  • Mesenchymal stem cell factor composition as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Preparation of MSC cytokine freeze-dried powder

[0049] 1.1 Collect MSC stem cells

[0050] Case collection: 6 cases of hypertrophic scar tissue (not receiving any drug treatment) surgically resected in the Affiliated Hospital of Nantong University, including 20 males and 20 females, aged 18-53 years, confirmed by histopathological examination. There were 40 normal skin cases of surgical patients in the same period, including 20 males and 20 females, aged 18-53 years old. Adult patients with non-hematological diseases underwent iliac bone marrow aspiration to extract bone marrow. All materials were obtained with the patient's informed consent and approved by the hospital ethics committee.

[0051] 1.2 Cell culture

[0052] 1.2.1 The main reagents and instruments used in the experiment of intervening fibroblasts with conditioned medium of bone marrow mesenchymal stem cells:

[0053]

[0054] 1.2.2 Fibroblast culture: Take two kinds of skin tissues, remove the epiderm...

Embodiment 2

[0062] A lotion containing mesenchymal stem cell factor freeze-dried powder, characterized in that it contains the following components by weight percentage: mesenchymal stem cell factor freeze-dried powder 2.0%, glycerol 1%, polyol A 1.5%, Betaine 1%, panthenol 0.5%, dipotassium glycyrrhizinate 0.05%, other active ingredients A 0.11%, polyethylene glycol-3 2.5%, surfactant A 0.2%, preservative A 0.4%, xanthan gum 0.15%, flavor 0.03%, and purified water 90.56%; wherein, the polyol A is butanediol; the other active ingredient A is nicotinamide and sodium hyaluronate, the ratio of the two is 1.1; The surfactant A is hydroxyethyl cellulose and PEG-40 hydrogenated castor oil, the ratio of the two is 1.1; the preservative A is methyl paraben. Prepare the lotion according to conventional methods in the art.

Embodiment 3

[0064] A facial mask essence containing mesenchymal stem cell factor freeze-dried powder, which is characterized in that it contains the following components in weight percentage: mesenchymal stem cell factor freeze-dried powder 4.5%, glycerin 1%, polyol B 3% , Panthenol 1%, Dipotassium Glycyrrhizinate 0.1%, Allantoin 0.1%, Other Active Ingredient B 2.0%, Surfactant B 3.6%, Preservative B 0.45%, Xanthan Gum 0.2%, Acrylic Resin Pemulen TR- 2 0.3%, fragrance 0.05%, and added aminomethylpropanol, the content of the aminomethylpropanol is based on adjusting the pH value of the facial mask essence to 6, and purified water 83%; the polyol Class B is 1,3-propanediol; the other active ingredients B are nicotinamide and sodium hyaluronate, the ratio of the two is 1.1; the surfactant B is hydroxyethyl cellulose, isopropyl isostearate , The mixture of polyglycerol-2 oleate, the ratio of the three is 1:1:1; the preservative B is methyl paraben. The facial mask essence is prepared accordin...

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Abstract

The invention belongs to the field of skin repair and provides mesenchymal stem cell factor freeze-dried powder as well as a preparation method and application thereof. The mesenchymal stem cell factor freeze-dried powder prepared by the method provided by the invention is high in purity, injuries of foreign additives on the human body and skin can be avoided, and potential hazards of skin allergy are avoided. The prepared freeze-dried powder product is stable in property, so that the biological activity of the mesenchymal stem cell factor freeze-dried powder is achieved for a long time.

Description

Technical field [0001] The invention belongs to the technical field of skin repair, and specifically relates to a mesenchymal stem cell factor and a preparation method and application thereof. Background technique [0002] The skin is the largest organ of the human body and covers the surface of the human body. It has the functions of protection, sensation, and regulation of body temperature. Its structure is from top to bottom, followed by epidermis, dermis and hypodermis. As people grow older, the signs of skin aging will gradually appear. The causes of skin aging can be divided into internal and external causes. [0003] Intrinsic aging refers to the natural aging process of the body, including the gradual decrease of keratinous stem cells as we age, resulting in basal cell disorders, reduced hyaluronic acid, and loss of moisturizing factors; decreased secretion of hormones (such as sebaceous glands) slows down the overall The metabolism of the skin ultimately makes the skin la...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61Q19/00A61Q19/08A61K8/34A61K8/44A61K8/42A61K8/63A61K8/86A61K8/73A61K8/49A61K8/37A61K8/92
CPCA61K8/022A61K8/34A61K8/345A61K8/37A61K8/42A61K8/44A61K8/4946A61K8/4993A61K8/63A61K8/64A61K8/73A61K8/731A61K8/735A61K8/86A61K8/922A61K2800/10A61Q19/00A61Q19/08
Inventor 王峰
Owner 陕西德健众普生物科技有限公司
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