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Electrochemical sensor for detecting mercury, its preparation method and application

An electrochemical and sensor technology, applied in the field of genes, can solve the problems of affecting DNA activity, easy to use environmentally harmful substances, unstable fixation, etc., to achieve simple production, improved high efficiency and sensitivity, good dispersion and biological phase. capacitive effect

Active Publication Date: 2020-03-17
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods all have defects such as weak fixation, the use of various affinity substances in fixation to affect the activity of DNA, and easy use of substances harmful to the environment.

Method used

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  • Electrochemical sensor for detecting mercury, its preparation method and application
  • Electrochemical sensor for detecting mercury, its preparation method and application
  • Electrochemical sensor for detecting mercury, its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0047] A kind of electrochemical sensor for detecting mercury of the present invention, see figure 1 : Including a glassy carbon electrode used as a working electrode in a three-electrode system, the surface of the detection end of the glassy carbon electrode is modified with carbon-doped graphene-like carbon nitride, and the carbon-doped graphene-like carbon nitride is deposited with Nano gold particles. Since there are available adsorption sites on the gold nanoparticles, add thiol-modified probe P1 on the glassy carbon electrode. site. Then add probe P2, part of the deoxyribonucleotide of probe P2 can form a double-stranded structure with the part of the deoxyribonucleotide of the probe P1 modified by the sulfhydryl group through thymine-mercury-thymine complementary pairing, and the probe P1 and The other part of the unpaired deoxyribonucleotides in the probe P2 are respectively paired with another thiol-modified probe P3 immobilized on the gold nanoparticles through bas...

Embodiment 2

[0057] A preparation method of the electrochemical sensor of embodiment 1, specifically comprises the following steps:

[0058] (1) Preparation of carbon-doped graphene-like carbon nitride:

[0059] Put 1 g of melamine in a reaction kettle with a volume of 100 mL, inject 80 mL of absolute ethanol, and react at a temperature of 200° C. for 24 h to obtain a colorless and transparent product. After drying the colorless and transparent product at room temperature, wrap it with tin foil, transfer it to a muffle furnace, raise the temperature to 520°C at a rate of 2°C / min, and calcinate at 520°C for 4h to obtain a carbon-doped graphene-like phase carbon nitride.

[0060] (2) Glassy carbon electrode pretreatment:

[0061] Polish the surface of the glassy carbon electrode (GCE), then rinse the surface of the glassy carbon electrode with water, then ultrasonically clean it with nitric acid, acetone, and ultrapure water, and finally use Tris-HCl buffer solution (Tris -HCl buffer solu...

Embodiment 3

[0074] A kind of application of the electrochemical sensor of embodiment 1 in detecting mercury ion, concrete detection method is:

[0075] (1) Probe P2 is respectively mixed with mercury ions of gradient concentration (mercury ion concentration is 10 -7 M, 5.0×10 -8 M, 1.0×10 -8 M, 5.0×10 -9 M, 1.0×10 -9 M, 5.0×10 -10 M, 1.0×10 -10 M, 5.0×10 -11 M, 2.0×10 -11 M, 1.0×10 -11 M) Mix well, the concentration of probe P2 is 1 μM.

[0076] (2) Probe P1 / nano-gold / carbon doped graphene-like phase carbon nitride modified glassy carbon electrode is soaked in the mixed solution of mercury and probe P2, so that probe P1, mercury and probe P2 react at room temperature for 30min .

[0077](3) The glassy carbon electrode obtained in step (2) was reacted with P3 immobilized on the surface of gold nanoparticles at 37° C. for 60 min (reaction time can be 30-90 min), and the concentration of probe P3 was 2 μM.

[0078] (4) Soak the glassy carbon electrode of the assembled electrochemi...

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Abstract

The invention provides an electrochemical sensor for detecting mercury as well as a preparation method and application of the electrochemical sensor. The electrochemical sensor comprises a glassy carbon electrode, a probe P2 and a mercapto-modified probe P3 which is fixed on gold nano-particles; the surface of the detection end of the glassy carbon electrode is modified with carbon-doped graphite-like carbon nitride; the gold nano-particles deposit on the carbon-doped graphite-like carbon nitride; a mercapto-modified probe P 1 is linked to the gold nano-particles; a part of deoxyribonucleotide of the probe P2 can be in complementary pairing with a part of deoxyribonucleotide of the mercapto-modified probe P1 to form a double-chain structure; and unpaired deoxyribonucleotide in the probe P1 and probe P2 and the mercapto-modified probe P3 which is fixed on the gold nano-particles form the double-chain structure. The preparation method comprises the following steps of modifying the graphite-like carbon nitride, modifying nanogold, modifying the probe P1 and the like. The electrochemical sensor provided by the invention has strong ability of resisting heavy metal ion interference and can be applied to detecting mercury ions.

Description

technical field [0001] The invention relates to the field of gene technology, in particular to an electrochemical sensor for detecting mercury and its preparation method and application. Background technique [0002] At present, the methods for determining pollutants in the environment mainly include chromatography, ultraviolet spectroscopy, synchronous fluorescence spectroscopy, spectrophotometry, derivative photometry, and flow injection analysis. These methods have defects such as complex pretreatment, long time consumption, large sample matrix effect, and long analysis cycle. They have high requirements on the operation level of instruments and staff, and it is difficult to popularize and apply them in small and medium-sized enterprises. For example, when using spectrophotometry to detect pollutants, due to the requirements for substrate turbidity and the influence of light-interfering substances, its accuracy and scope of use are limited; while liquid and gas chromatogr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 竺园曾光明章毅汤琳张立华安鸿雪黄真真
Owner HUNAN UNIV
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