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Bacterial cellulose nanofiber composite membrane for efficient protein adsorption and separation and preparation method of composite membrane

A technology of bacterial cellulose and nanofibers, which is applied in fiber types, fiber treatment, plant fibers, etc., can solve the problems of limited specific surface area of ​​electrospun fibers, difficulty in practical application, and increased protein adsorption. The effect of short time consumption and increased adsorption capacity

Active Publication Date: 2017-12-01
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent "graphene-based protein adsorption sponge" (CN106622120A) discloses a preparation method of graphene-based protein adsorption sponge, which can be used for protein adsorption after functional group modification of graphene oxide, solvent replacement, freeze-drying and high-temperature calcination. Graphene sponge, but this method is costly and time-consuming (period > 5 days), making it difficult to achieve practical application
文献[Scalablefabrication of electrospun nanofibrous membranes functionalized with citricacid for high-performance protein adsorption[J].ACS Applied Materials&Interfaces,2016,8,11819-11829]和[Highly carbonylated cellulose nanofibrousmembranes utilizing maleic anhydride grafting for efficient lysozymeadsorption[J].ACS Applied Materials & Interfaces, 2015, 7, 15658-66] reported a method of modifying adsorption functional groups on the surface of electrospun fiber membranes to prepare protein adsorption and separation membranes. The high porosity of electrospun fiber membranes can be used to achieve rapid mass transfer of proteins , but due to the limited specific surface area of ​​electrospun fibers (diameter > 200nm), it is difficult to achieve a substantial increase in protein adsorption

Method used

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  • Bacterial cellulose nanofiber composite membrane for efficient protein adsorption and separation and preparation method of composite membrane
  • Bacterial cellulose nanofiber composite membrane for efficient protein adsorption and separation and preparation method of composite membrane

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Effect test

Embodiment 1

[0029] A preparation method of bacterial cellulose nanofiber composite membrane for efficient protein adsorption and separation:

[0030] Step 1: Mechanically dissociate the bacterial cellulose membrane into bacterial cellulose nanofibers with an average length of 300 μm and an average diameter of 100 nm by high-speed stirring and dissociation;

[0031] Step 2: modifying the carboxylic acid group (-COOH) on the surface of the bacterial cellulose nanofiber;

[0032] Step 3: the above-mentioned bacterial cellulose nanofibers are dispersed in water, and a stable bacterial cellulose nanofiber suspension is formed by adding a dispersant alkylphenol polyoxyethylene ether; the mass percentage of bacterial cellulose nanofibers in the suspension is 0.05wt%;

[0033] Step 4: Apply simultaneous ultrasonic filtering (eg figure 1 Shown) method spreads above-mentioned bacterial cellulose nanofiber suspension on the cellulose filter paper surface that pore size is 300 μm to form wet state ...

Embodiment 2

[0036] A preparation method of bacterial cellulose nanofiber composite membrane for efficient protein adsorption and separation:

[0037] Step 1: Mechanically dissociate the bacterial cellulose membrane into bacterial cellulose nanofibers with an average length of 180 μm and an average diameter of 80 nm by ultrasonic dissociation;

[0038] Step 2: Modification of sulfonic acid groups (-SO 3 H);

[0039] Step 3: the above-mentioned bacterial cellulose nanofibers are dispersed in methanol, and a stable bacterial cellulose nanofiber suspension is formed by adding a dispersant fatty alcohol polyoxyethylene ether; the mass percentage of bacterial cellulose nanofibers in the suspension is 0.002wt%;

[0040]Step 4: spread the above-mentioned bacterial cellulose nanofiber suspension on the surface of a polyethylene terephthalate spunbond nonwoven fabric with a pore size of 150 μm to form a wet composite fiber membrane by means of synchronous ultrasonic filtration; the synchronous ul...

Embodiment 3

[0043] A preparation method of bacterial cellulose nanofiber composite membrane for efficient protein adsorption and separation:

[0044] Step 1: Mechanically dissociate the bacterial cellulose membrane into bacterial cellulose nanofibers with an average length of 100 μm and an average diameter of 50 nm by using a high-pressure homogeneous dissociation method;

[0045] Step 2: modifying sulfate groups (-OSO) on the surface of the bacterial cellulose nanofibers 3 H);

[0046] Step 3: the above-mentioned bacterial cellulose nanofibers are dispersed in ethanol, and a stable bacterial cellulose nanofiber suspension is formed by adding the dispersant sodium stearate; the mass percentage of bacterial cellulose nanofibers in the suspension is 0.001wt %;

[0047] Step 4: spread the above-mentioned bacterial cellulose nanofiber suspension on the surface of a polypropylene melt-blown nonwoven fabric with a pore size of 80 μm by synchronous ultrasonic filtration to form a wet composite...

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Abstract

The invention discloses a bacterial cellulose nanofiber composite membrane for efficient protein adsorption and separation and a preparation method of the composite membrane. The preparation method comprises the steps as follows: a bacterial cellulose membrane is mechanically dissociated into bacterial cellulose nanofiber; the surface of the bacterial cellulose nanofiber is modified with an adsorption functional group; the modified bacterial cellulose nanofiber is dispersed in an insoluble solvent, and a stable bacterial cellulose nanofiber suspension is formed by adding a dispersant; the bacterial cellulose nanofiber suspension is laid on the surface of a porous fiber base material with a synchronous ultrasonic filtration method to form a wet-state composite fiber membrane; the residual solvent in the wet-state composite fiber membrane is removed and the bacterial cellulose nanofiber composite membrane for efficient protein adsorption and separation can be obtained. A completely covered continuous two-dimensional net structure formed by the bacterial cellulose nanofiber is arranged on the surface of the composite membrane, meanwhile, a large quantity of adsorption active sites on the surface and high porosity are realized, and quick and effective adsorption of protein is realized, and the adsorption capacity is high.

Description

technical field [0001] The invention relates to a bacterial cellulose nanofiber composite membrane for efficient protein adsorption and separation and a preparation method thereof, in particular to a bacterial cellulose nanofiber composite membrane capable of fast protein adsorption and separation and a preparation method thereof, belonging to nanofiber composite membranes field of materials technology. Background technique [0002] Realizing efficient separation and purification of proteins is of great significance in the fields of biopharmaceuticals, analytical chemistry, and life sciences. The current main protein separation and purification technologies include centrifugation, precipitation, chromatography, electrophoresis, etc. Among them, chromatography technology is the most widely used in protein separation and purification due to its advantages of good selectivity, high separation efficiency, and strong applicability. At present, the protein adsorption and separati...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/28B01J20/30B01D71/12B01D69/12B01D67/00D06M15/05D06M101/32D06M101/20D06M101/06D06M101/28D06M101/34
CPCB01D67/0002B01D69/12B01D71/12B01D2325/02B01J20/265B01J20/2808B01J20/28095D06M15/05D06M2101/06D06M2101/20D06M2101/28D06M2101/32D06M2101/34
Inventor 丁彬唐宁张世超刘丽芳俞建勇
Owner DONGHUA UNIV
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