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Visual method for detecting electroactivity of biological film

A detection method and biofilm technology, which can be used in measurement devices, material electrochemical variables, instruments, etc., can solve the problems of being unsuitable for long-term observation, prone to photobleaching, unable to reflect the electrical activity of biofilms, etc. Observation, good biocompatibility, fluorescence lifetime and anti-photobleaching effect

Active Publication Date: 2017-12-12
天泓(济南)智能装备产业研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, laser confocal microscopy is commonly used to characterize biofilms with organic fluorescent dyes, which are prone to photobleaching and are not suitable for long-term observation. They are only suitable for the analysis of dead and alive bacteria, and cannot reflect the electrical activity of biofilms.

Method used

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  • Visual method for detecting electroactivity of biological film
  • Visual method for detecting electroactivity of biological film
  • Visual method for detecting electroactivity of biological film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The method for in situ detection of biofilm electroactivity comprises the following steps:

[0053] 1) A 100mL screw-top glass bottle was used as a single-chamber reactor cell, ITO conductive glass was used as a working electrode, a saturated calomel electrode was used as a reference electrode, and a titanium wire was used as an auxiliary electrode to construct a three-electrode system reactor;

[0054] 2) To the reactor assembled in step 1), add 30mL of microbial fuel cell culture solution for high-efficiency electricity production and containing electricity-producing microorganisms; add 60mL of sodium acetate medium as an electron donor;

[0055] Preferably, the microbial fuel cell culture fluid that comprises electricity-producing microorganisms refers to: adding carbon source to the microbial fuel cell is the microbial fuel cell liquid culture medium of sodium acetate and inoculating the electricity-producing microorganism Hiddenella (Geobacter), and then running the...

Embodiment 2

[0068] The method is the same as in Example 1, except that the working electrode is carbon cloth. The result is as Figure 5 As shown, a green fluorescent signal was observed on the surface of the carbon cloth.

[0069] It shows that this method can be used to directly detect the electrical activity of the biofilm with carbon cloth as the carrier.

Embodiment 3

[0071] The method is the same as in Example 1, except that the precursor compound 1 is sodium tellurite and cysteine, and the precursor compound 2 is cadmium acetate, cadmium nitrate, zinc chloride, and zinc acetate.

[0072] Electroactive biofilms were synthesized to obtain fluorescent quantum dots of cadmium telluride, cadmium sulfide, zinc telluride and zinc sulfide, respectively.

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Abstract

The invention discloses a visual method for detecting the electroactivity of a biological film. The method comprises the following steps: combining a biological film fuel battery with quantum dots; selecting IIB-VIA group elements as precursor compounds; reducing the precursor compound 1 by using the electron transfer capability of the biological film; combining the reduced precursor compound 1 with metal ions of the precursor compound 2 so as to obtain semiconductor nano particles, namely the quantum dots. The ability of the biological film synthesized quantum dots is related to electroactivity thereof, and therefore, the fluorescence intensity of the synthesized quantum dots can visually reflect the electroactivity of the biological film.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to an intuitive detection method for biofilm electrical activity. Background technique [0002] Biofilm, also known as biofilm, refers to an organized bacterial population attached to the surface of a solid carrier and wrapped by bacterial extracellular polymers. The formation process of biofilm mainly includes bacterial initial adhesion, biofilm growth and mature diffusion. [0003] The biofilm reactor is formed by introducing biofilm into the sewage treatment reactor of urban domestic wastewater treatment through artificial reinforcement. The biofilm reactor is used for the secondary biological treatment of urban sewage treatment plants. It has the advantages of stable operation, strong impact resistance, economical and energy saving, certain nitrification and denitrification functions, and can realize closed operation to prevent odor. [0004] Biofilm degradation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N27/26
CPCG01N21/6458G01N27/26
Inventor 温俊林周顺桂余震陈俊华杨贵芹汤佳
Owner 天泓(济南)智能装备产业研究有限公司