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Sulfur-doped carbon quantum dot fluorescence sensitization method for testing norfloxacin

A technology of carbon quantum dots and norfloxacin, which is applied in the field of sulfur-doped carbon quantum dots for fluorescence-enhanced detection of norfloxacin, achieving high sensitivity, specificity, high sensitivity, and high fluorescence quantum yield

Active Publication Date: 2017-12-26
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, carbon quantum dots doped with sulfur or co-doped with nitrogen and sulfur are rarely reported, and the effect of sulfur on carbon quantum dots needs further research.

Method used

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  • Sulfur-doped carbon quantum dot fluorescence sensitization method for testing norfloxacin
  • Sulfur-doped carbon quantum dot fluorescence sensitization method for testing norfloxacin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the content determination operation steps of norfloxacin in the blood are as follows:

[0024] (1) Preparation of water-soluble sulfur-doped fluorescent carbon quantum dots (S-CQDs): Weigh 2.0g sodium poly(4-styrenesulfonic acid-copolymerization-maleic acid) into 100mL ultrapure water, and ultrasonicate for 5min Form a colorless and transparent solution, transfer it to a polytetrafluoroethylene reactor, heat at 220°C for 5 h, and after natural cooling, first filter it with a filter membrane with a pore size of 0.22 μm, and then perform dialysis treatment with a dialysis bag with a molecular weight cut-off of 3000 Da for 24 h , to obtain water-soluble sulfur-doped fluorescent carbon quantum dots;

[0025] (2) Drawing of the standard working curve: 8 norfloxacin with concentrations of 0.01, 0.05, 0.1, 0.2, 0.5, 1, 5, 10 μmol / L were prepared using pH5.0 citric acid-disodium hydrogen phosphate buffer solution Standard solution, and set the volume at 5 mL; add...

Embodiment 2

[0028] Embodiment 2: the content determination steps of norfloxacin in the urine sample are:

[0029] (1) Preparation of water-soluble sulfur-doped fluorescent carbon quantum dots: The preparation method of water-soluble sulfur-doped fluorescent carbon quantum dots is to weigh 3.0 g poly(4-styrenesulfonic acid-copolymerization-maleic acid) sodium and add 100mL of ultrapure water, ultrasonic 8min to form a colorless transparent solution, and transferred to a polytetrafluoroethylene reactor, heated at 200 ℃ for 8h, after natural cooling, first filter with a filter membrane with a pore size of 0.22μm, and then filter with a molecular weight cut-off of 3500Da The dialysis bag was dialyzed for 24 hours to obtain water-soluble sulfur-doped fluorescent carbon quantum dots;

[0030] (2) drawing of standard working curve: with embodiment 1 step (2);

[0031] (3) Urine sample preparation: Take 5 mL of urine, add 1 g of activated carbon, vortex and mix for 1 min, let it stand and filter...

Embodiment 3

[0033] Embodiment 3: the content determination steps of norfloxacin in the milk sample are:

[0034] (1) Preparation of water-soluble sulfur-doped fluorescent carbon quantum dots: The preparation method of water-soluble sulfur-doped fluorescent carbon quantum dots is to weigh 5.0 g poly(4-styrenesulfonic acid-copolymerization-maleic acid) sodium and add 100mL of ultrapure water, ultrasonic 10min to form a colorless transparent solution, and transferred to a polytetrafluoroethylene reactor, heated at 240 ℃ for 6h, after natural cooling, first filter with a filter membrane with a pore size of 0.22μm, and then filter with a molecular weight cut-off of 3200Da The dialysis bag was dialyzed for 24 hours to obtain water-soluble sulfur-doped fluorescent carbon quantum dots.

[0035] (2) drawing of standard working curve: with embodiment 1 step (2);

[0036] (3) Milk sample preparation: Take 5 mL of milk, add 10 mL of methanol-water-formic acid (28:72:0.1, v / v) extract and 1 mL of 4% tr...

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Abstract

The invention discloses a sulfur-doped carbon quantum dot fluorescence sensitization method for testing norfloxacin. The method employs poly(4-styrene sulfonic acid-copolymerization-maleate)sodium as a carbon source and a sulfur source, a hydrothermal method is employed for synthesizing the sulfur-doped carbon quantum dot; the sulfur-doped carbon quantum dot can enhance the fluorescence of norfloxacin, the enhanced fluorescence signal intensity and the norfloxacin concentration show a linear relationship, so that a fluorospectrophotometry for detecting the norfloxacin is established. The sulfur-doped carbon quantum dot has obvious fluorescence sensitization effect on the norfloxacin, has strong selectivity, and has the characteristics of high sensitivity and specificity for fluorescence determination for the norfloxacin.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for fluorescence-sensitized detection of norfloxacin by sulfur-doped carbon quantum dots. Background technique [0002] Norfloxacin, commonly known as norfloxacin, has a broad antibacterial spectrum and strong antibacterial activity. It is an important synthetic drug for the treatment of various infectious diseases and is widely used clinically. At present, the analysis methods of norfloxacin mainly include flow injection photometry, ultraviolet-visible photometry, fluorescence photometry, and analysis methods using quantum dots (Quantum Dot, QDS) as fluorescent probes, etc. Quantum dots have the advantages of wide excitation band, narrow half-peak width, symmetrical and adjustable emission band, less prone to photobleaching, high fluorescence quantum yield, quantum size effect, and surface effect. In the article "Determination of Norfloxacin Con...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 杨亚玲华建豪杨德志
Owner KUNMING UNIV OF SCI & TECH
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