Multiple species inoculant and application thereof in fermenting organic waste to increase breeding of indigenous microbes to produce bio-organic fertilizer

A compound bacterial agent and fermentation tank technology, applied in the field of microorganisms and the environment, can solve the problems of high cost, single function, and inability to mix and ferment different organic wastes, and achieve the effect of saving resources and solving pollution

Inactive Publication Date: 2018-01-19
中山国晟生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to, aiming at the above-mentioned deficiencies in the prior art, provide a kind of composite bacterial agent, solve the single function of existing microbial fermentation agent, poor effect, high cost, be used for fermenting biological fertilizer and need to add repeatedly, fermentation is not thoroughly decomposed, Issues such as low level of harmlessness
[0009] The invention solves the technical problem that the prior art cannot realize the mixed fermentation of different organic wastes. By adopting the technical scheme of the invention, it can realize the common method of mixing different solid organic wastes or solid-liquid mixed organic matter in any proportion as fermentation raw materials. Fermentation realizes the harmless and efficient comprehensive utilization of organic waste, solves the pollution of organic waste to the environment, saves resources, is economical and efficient, and is ecologically friendly

Method used

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  • Multiple species inoculant and application thereof in fermenting organic waste to increase breeding of indigenous microbes to produce bio-organic fertilizer
  • Multiple species inoculant and application thereof in fermenting organic waste to increase breeding of indigenous microbes to produce bio-organic fertilizer
  • Multiple species inoculant and application thereof in fermenting organic waste to increase breeding of indigenous microbes to produce bio-organic fertilizer

Examples

Experimental program
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Effect test

Embodiment 1

[0084] Embodiment 1 adopts the following steps to prepare the composite bacterial agent of the present invention

[0085] 1. Preparation of pure dry powder of Bacillus carbonophilus:

[0086] (1) Activation culture of Bacillus carbonophilus: Inoculate the slant strain of Bacillus carbonophilus into the activation culture shake flask for activation culture. The activation medium formula is: beef extract 2.05g / L, peptone 0.45g / L, chloride Sodium 0.15g / L, yeast extract 1.40g / L, activated carbon powder 0.33g / L, pH 7.0; the activation culture conditions are: shake flask rotation speed 190rpm, culture at 29-30℃ for 22h to obtain liquid fermented seeds;

[0087] (2) Expansion culture and dehydration of Bacillus arborophilus: inoculate the obtained liquid fermented seeds into the expansion culture fermenter, the inoculation amount is 8.5%, the expansion medium formula is: maltose 30g / L, corn flour 16g / L, Yeast extract 4.8g / L, urea 3.0g / L, K 2 HPO 4 0.15g / L, MgSO 4 ·7H 2 O 0.015g...

Embodiment 2

[0113] Embodiment 2 adopts the pure thalline dry powder of 9 kinds of microbial bacteria that step 1-9 of embodiment 1 makes, according to the following ratio of the number of live bacteria in the pure thallus dry powder to the total number of live bacteria in the composite bacterial agent: Bacillus arborophilus 3 %, Bacillus amyloliquefaciens 5%, Geobacillus stearothermophilus 10%, Bacillus subtilis 30%, Streptomyces knowlesii 10%, actinomycetes fermentative 15%, Penicillium oxalicum 10%, Hansen 7% of Debaria yeast, 10% of Azotobacter rotundum, and the pure dry powder of 9 kinds of microbial bacteria are evenly mixed to prepare a composite bacterial agent.

Embodiment 3

[0114] Embodiment 3 adopts the pure thalline dry powder of 9 kinds of microbial bacteria that step 1-9 of embodiment 1 makes, according to the following ratio of the number of live bacteria in the pure thallus dry powder to the total number of live bacteria in the composite bacterial agent: Bacillus arborophilus 5 %, Bacillus amyloliquefaciens 9%, Geobacillus stearothermophilus 20%, Bacillus subtilis 15%, Streptomyces knowlesii 5%, actinomycetes fermentative 12%, Penicillium oxalicum 8%, Hansen 11% of Debaria yeast, 15% of Azotobacter rotundum, and the pure dry powder of 9 kinds of microorganisms are evenly mixed to prepare a composite bacterial agent.

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Abstract

The invention discloses a multiple species inoculant and an application thereof in fermenting organic waste to increase breeding of indigenous microbes to produce a bio-organic fertilizer. The multiple species inoculant is prepared from carbophilus bacillus, bacillus amyloliquefaciens, thermophilus fat bacillus, bacillus subtilis, streptomyces noursei, fermenting paying-off fibril bacterium, penicillium oxalicum, Hasun debaryomyces and azotobacter chroococcum by adopting the processes of strain activation, amplification cultivation and dewatering and drying to obtain pure thallus dry powder ofvarious strains, and then uniformly mixing the pure thallus dry powder according to a proportion; the multiple species inoculant is used for fermenting organic waste to produce a bio-organic fertilizer, and protogene beneficial bacteria in the ambient are activated during fermentation, so that mass propagation of the population of beneficial bacteria is achieved, and after fermentation is over, effective active bacteria enzyme activity and the quantity of population of the beneficial bacteria are high, further adding of functional microorganisms is not needed, and the bio-organic fertilizer can be transformed at one time directly. According to the invention, joint fermentation of solid organic waste or solid-liquid organic matters which have different sources and are mixed in any proportion can be realized.

Description

technical field [0001] The invention relates to a microbial bacterial agent and its application, in particular to a composite bacterial agent and its application in the production of bio-organic fertilizer by proliferating the original bacteria in fermenting organic waste, and belongs to the technical field of microorganisms and the environment. Background technique [0002] Although my country's agricultural ecological environment construction and protection have made great achievements, in recent years, my country's agricultural environment and agricultural product pollution problems have become increasingly serious, the environmental quality of cultivated land has continued to decline, and the problem of toxic and harmful substance residues in agricultural products has become prominent, which has become a constraint for agriculture and rural areas. important factor in economic development. [0003] The pollution of my country's industrial "three wastes" to the agricultural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F15/00C12R1/07C12R1/125C12R1/57C12R1/01C12R1/80C12R1/645
Inventor 凌仕信
Owner 中山国晟生物工程有限公司
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