Fig soy sauce
A technology of figs and soy sauce, which is applied in the direction of yeast-containing food ingredients, food ingredients as odor improvers, and the functions of food ingredients. effect of effect
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Embodiment 1
[0020] Embodiment 1: the screening of bacterial strain
[0021] 1. Strains: Aspergillus isolated from rotten figs by natural selection. The bacterium has strong ability to decompose cellulose, has high protease activity and low pectinase activity, high juice yield and high utilization rate of raw materials.
[0022] 2. Culture medium and detection method:
[0023] (1) Potato glucose liquid medium: 200g potato (peeled), 20g glucose, 1000mL distilled water, natural pH. Sterilize at 121°C for 20 minutes.
[0024] (2) Potato dextrose agar medium: 200g potato (peeled), 20g glucose, 20g agar, 1000mL distilled water, natural pH. Sterilize at 121°C for 20 minutes.
[0025] (3) Fermentation medium: 30% fig pulp, sterilized at 80° C. for 15 minutes.
[0026] (4) Determination of enzyme activity: Determination of pectinase enzyme activity - sodium hypochlorite method.
[0027] Determination of protease enzyme activity - Folin (Folin) phenol reagent method.
[0028] Determination o...
Embodiment 2
[0034] Example 2: Characterization of Aspergillus FJ036
[0035] (1) Form
[0036] Aspergillus FJ036 was inoculated on a potato dextrose agar medium plate, and cultured at 28° C. for 3 days, the colony diameter reached 4 cm. The colonies spread out in the shape of concentric rings, and the hyphae grow prostrate and are white. After the formation of spores, the colonies turn black. The color of the medium has no obvious change, no obvious exudate, and a musty smell. The top of the conidia head forms a spherical apical capsule, which is covered with a layer of stalk base and a layer of small stalks. There are clusters of brown-black spherical spores on the small stalks, which are Aspergillus.
[0037] (2) Determination of genetic stability
[0038] Table 2: Aspergillus FJ036 carries out serial passage genetic stability result
[0039]
[0040] Aspergillus FJ036 was subcultured for 10 consecutive generations, each generation was fermented separately, and its cellulase, prot...
Embodiment 3
[0041] The preparation of embodiment 3 fig fermented liquid
[0042] Described fig fermented liquid, its preparation method is as follows:
[0043] 1) After the fresh figs are blanched, the blanched figs are broken and beaten to make a slurry;
[0044] 2) insert Aspergillus FJ036 strain into the sterilized fig pulp, and carry out fermentation culture; after the fermentation, centrifuge to obtain Aspergillus FJ036 enzyme fermentation liquid;
[0045] 3) adding the Aspergillus FJ036 enzyme fermentation liquid into the sterilized fig pulp, and performing enzymatic hydrolysis treatment; after the enzymatic hydrolysis, the enzyme was inactivated to obtain the fig fermentation liquid.
[0046] Table 3: Comparison of treatment results of fig fermentation broth
[0047]
[0048]
[0049]It can be known from Table 3 that the cellulase and protease activities of Aspergillus FJ036 strain were 51.2±1.5U / mL and 28.2±0.6U / mL, which were 27.0% and 18.5% higher than Aspergillus niger,...
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