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A surface-enhanced Raman spectroscopy detection platform and its preparation method and application

A surface-enhanced Raman and spectral detection technology, applied in the field of nanomaterials, can solve the problem of no polydopamine, etc., and achieve the effects of wide linear response detection range, simple preparation, and mild reaction conditions

Active Publication Date: 2020-10-30
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, polydopamine has been applied to the modification and modification of labeling materials by more and more researchers in recent years, but there is no use of polydopamine for the quantitative detection of substrate modification, and the quantification of trace biomarkers with combined labeling materials detection report

Method used

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  • A surface-enhanced Raman spectroscopy detection platform and its preparation method and application
  • A surface-enhanced Raman spectroscopy detection platform and its preparation method and application
  • A surface-enhanced Raman spectroscopy detection platform and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Preparation of polydopamine-modified slide chip (PDA-chip)

[0035] Before preparation, soak slides in piranha water (concentrated sulfuric acid: hydrogen peroxide solution = 7:3), soak overnight, wash, dry and cool to room temperature. Next, put the glass slide into a clean petri dish and place it on a constant temperature shaker, add 20mL of 10mg / mL dopamine hydrochloride solution, adjust the speed of the constant temperature shaker, shake and react at 37°C for 1 hour, then add 20mL of 10mM pH 8.5 tris-hydrochloric acid (Tris-HCl) buffer solution and 200uL of 15ug / mL glypican 1 (GPC1) antibody solution, continue shaking reaction at 37°C for 1 hour, the solution is prepared by When it becomes clear and transparent to gray-black, a polydopamine-modified glass slide chip is generated, and then rinsed with phosphate buffered saline, then soaked in 0.5% bovine serum albumin solution for 1 hour, and then washed with deionized water and phosphate buffered saline Liquid ...

Embodiment 2

[0043] (1) Preparation of polydopamine-modified slide chip (PDA-chip)

[0044] Before preparation, soak slides in piranha water (concentrated sulfuric acid: hydrogen peroxide solution = 7:3), soak overnight, wash, dry and cool to room temperature. Next, put the glass slide into a clean petri dish and place it on a constant temperature shaker, add 30mL of 10mg / mL dopamine hydrochloride solution, adjust the speed of the constant temperature shaker, shake and react at 37°C for 1 hour, then add 30mL of 10mM pH 8.5 tris-hydrochloric acid (Tris-HCl) buffer solution and 300uL of 15ug / mL GPC1 antibody solution, continue shaking reaction at 37°C for 1 hour, the solution changes from clear and transparent to gray-black, and the The polydopamine-modified slide chip was rinsed with phosphate buffered saline, soaked in 0.5% bovine serum albumin solution for 1 hour, washed with deionized water and phosphate buffered saline, and set aside.

[0045] (2) Preparation of polydopamine-modified s...

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Abstract

The present invention belongs to the technical field of nanometer materials, and particularly relates to a surface enhanced Raman spectroscopy detection platform, a preparation method and applicationsthereof, wherein the surface enhanced Raman spectroscopy detection platform comprises a polydopamine-modified substrate chip and a polydopamine-modified surface-enhanced Raman spectroscopy labeled SERS probe. The preparation method comprises: respectively placing a clean substrate chip and a SERS probe material in a dopamine solution containing targeting recognition molecule by using the self-polymerization reaction of dopamine, and soaking the substrate in a bovine serum albumin solution so as to respectively obtain a PDA chip having targeting capture ability and a SERS probe. According to the present invention, a solution containing a detected material is placed on the chip, the SERS probe interacts with the chip, and the concentration of the detected material can be detected by detecting the SERS signal of the probe; and with the application of the surface enhanced Raman spectroscopy detection platform to detect, the characteristics of rapidness, high efficiency, high specificity,high sensitivity, wide application range and high visualization are provided.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a surface-enhanced Raman spectrum detection platform and a preparation method and application thereof. Background technique [0002] The excellent luminescent properties of surface-enhanced Raman spectroscopy have attracted extensive attention from researchers in materials science, chemical biology, and life sciences in recent years. In the past five years, many researchers have done a lot of research on the functional modification methods of surface-enhanced Raman spectroscopy labels. Surface-enhanced Raman spectroscopy labeling materials have the characteristics of high brightness, no photobleaching and narrow spectral bandwidth. They are ideal probe materials for trace substance detection and biological imaging applications, and have great application value in the field of life sciences. [0003] Dopamine (DA) is a catechol-like neurotransmitter, a chemical ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 张人陈惠李腾达黄志鹏孔继烈
Owner FUDAN UNIV