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A strain of marine pseudoalteromonas and its decolorization flocculant preparation method

A pseudoalteromonas, decolorizing flocculant technology, applied in bacteria, chemical instruments and methods, microorganism-based methods, etc., to achieve the effect of high separation rate, stable performance, and no potential toxicity

Active Publication Date: 2020-01-21
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

General Microbiology Center (CGMCC), China Committee for Culture Collection of Microorganisms, is located at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A marine Pseudoalteromonas strain was isolated and screened from the spitting mud of Philippine clams cultured in a marine farm in Zhujiajian, Zhoushan, Zhejiang, China, and was identified as Pseudoalteromonas ( Pseudoalteromonas sp.), was deposited on August 29, 2016 in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microbial Cultures (CGMCC), and the preservation number is CGMCC No.12913. The General Microorganism Center (CGMCC) of China Committee for Culture Collection of Microorganisms is located at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0022] The screening method for a strain of marine pseudoalteromonas is as follows: collect fresh Philippine clams, put them into sterile water and spit out mud, take 1ml of mud, use gradient dilution to pour plate separation and streak purification, and screen out those with high flocculation by activity test and depigmenting active strains Pseudoalteromonas sp. G...

Embodiment 2

[0030] A method for preparing a decolorizing flocculant of marine pseudoalteromonas, comprising the following steps:

[0031] 1) Seed liquid culture: first inoculate the marine bacteria with the preservation number CGMCC No. 12913 on the slant medium Pseudoalteromonas sp. GHS18, and cultured at 22°C for 72 hours to activate the strain, then add sterile water 1.3 times the volume of the medium, and oscillate to obtain a seed solution;

[0032] 2) Preparation of liquid medium: prepare liquid medium according to the following ingredients and concentrations: rice straw hydrolyzate 250ml / L, ammonium sulfate 1.0 g / L, yeast extract 0.6g / L, dipotassium hydrogen phosphate 4.1g / L, Potassium dihydrogen phosphate 1.2g / L and the rest artificial seawater; adjust the pH to 7.4, and autoclave at 115°C for 30min. Among them, the artificial seawater formula is: sodium chloride 25.0 g / L, magnesium chloride 1.9 g / L, magnesium sulfate 3.1 g / L, calcium chloride 1.3 g / L, sodium bicarbonate 0.2 g / L,...

Embodiment 3

[0036] Example 3: Determination of flocculation activity

[0037] Adopt flocculation activity test method (R.Kurane, K.Takeda, T.Suzuki.Screening and Characteristics of Microbial Flocculants.Agric.Biol.Chem.1986: 2301-2307) to measure the flocculation activity of kaolin suspension, take 5g / L of kaolin suspension 93ml, add 5ml 1% (m / v) CaCl 2 As a coagulation aid, add 2ml of the sample of Example 1, adjust the pH value to 9, mix and stir quickly for 1min, then slowly stir for 5min, let stand for 10min, take the mixed solution at 1cm below the water level, and measure the absorbance A at 550nm with a visible spectrophotometer , while using 2ml of distilled water instead of the sample as a control, measure the absorbance B, and calculate the flocculation rate, FR(%) = (A-B) / A×100%. Then the flocculation rate of the flocculant to the kaolin suspension is 91.87%.

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Abstract

The invention discloses a strain of marine pseudoalteromonas and a method for preparing a decolorizing flocculant thereof. The marine pseudoalternomonas is obtained by separating and screening from the spitting mud of Philippine clams cultured in a seawater farm in Zhujiajian, Zhoushan, Zhejiang, China. , identified as Pseudoalteromonas ( Pseudoalteromonas sp.). The decolorizing flocculant preparation method includes seed liquid culture, liquid medium preparation, expanded fermentation culture, and flocculant preparation. The present invention uses inorganic acid to hydrolyze crop waste to obtain hydrolyzate to replace the carbon source in the culture medium, which saves costs; and uses sandy soil as the carrier to attach the bacteria to the carrier, so that the active ingredients of the flocculant can be easily and thoroughly cultivated separated from the liquid. The beneficial effect is that the prepared decolorizing flocculant has less dosage, high decolorizing rate, large floccules formed, fast settling speed, stable performance, good decolorizing and flocculating effect, no potential toxicity to organisms, and is green and healthy.

Description

technical field [0001] The invention relates to the field of preparation methods of microbial flocculants, in particular to a strain of marine pseudoalteromonas and a preparation method of a decolorizing flocculant thereof. Background technique [0002] Flocculation technology is widely used in water supply, wastewater flocculation purification, fermentation industry, solid-liquid separation, etc. The flocculation effect depends on the type of flocculant selected. At present, there are two main types of flocculants used in the water treatment industry: inorganic flocculants represented by aluminum-based, iron-based and their polymers, and organic polymer flocculants represented by PAM and their derivatives. They are widely used due to their good flocculation effect and low cost during use, but their unsafety to the environment has attracted enough attention. Aluminum ions in aluminum-based flocculants can easily cause Alzheimer's disease; iron ions in iron-based flocculants...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04C02F3/34C12R1/01
CPCC02F3/34C12P19/04C12P2203/00C12N1/205C12R2001/01
Inventor 穆军杨桥崔霞王东方王玉霞
Owner ZHEJIANG OCEAN UNIV
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