Preparation method of naringin

Abstract

The invention discloses a preparation method of naringin. An alkali extraction and acid deposition method is adopted, young grapefruits are selected as a raw material, lime and NaOH are added in a certain solid-liquid ratio, stirring and extraction are performed at the normal temperature, the pH value of an extraction solution is adjusted with HCl acid, filtration is performed after flocculation treatment with CaCl2, filtrate obtained after filtration passes through a ceramic membrane system, an obtained dialysate is subjected to acid adjustment, crystals can be quickly separated under the stirring condition and dried, and the product naringin is obtained; naringin is recovered from a nanofiltration concentrated solution obtained after an acid dialysate is subjected to nanofiltration membrane treatment, and the nanofiltration dialysate is recycled for continuous raw material extraction. Macroporous resin adsorption and recrystallization are not required, the purity of naringin can reach 98% or above, the extraction rate is high, the yield of naringin is high, the product purity is high, steps of macroporous resin adsorption separation and recrystallization in traditional technologies are omitted, the process is simplified, the production cycle is shortened, besides, the amount of wastewater is greatly reduced, and the method is suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a preparation method of naringin. Background technique [0002] Rutaceae pomelo is one of the most widely cultivated fruit trees in subtropical regions of my country. Pomelo fruit trees are affected by physiological factors, natural environment and other factors. Every year, 80% of the fruit on the tree will be artificially thinned or fall off naturally. Thousands of tons of young fruits are discarded in the orchard every year to rot, causing ecological pollution and waste of resources. Yang Shuang et al. found that during the growth period of the fruit, the naringin content decreased with the prolongation of the development time, and the content of naringin in the young fruit stage of various varieties was the highest (the content of "late white pomelo" can be as high as 35.29%). After the fruit is ripe, 92% of naringin is concentrated in the peel, and 8% is distributed in the pul...

AI Technical Summary

Problems solved by technology

Among them, the hot water extraction method is mainly carried out based on the principle that naringin has a high solubility in hot water, and a certain amount of hot water is used to extract the raw materials multiple times, so as to ensure that the naringin in the raw materials is completely extracted. During the extraction process, Impurities that are easily soluble in hot water, such as pectin, polysaccharides, and pigments, will be extracted from the raw materials by hot water together with naringin. The presence of a large number of impurities in the extract makes it difficult to separate naringin from the extract, resulting in crystallization. The time is long and the yield is low. If it is necessary to prepare naringin with a content of more than 98%, it needs to be adsorbed by a macroporous resin column, and recrystallized with alcohol and water for many times. At the same time, due to the water extraction method, a large amount of heat energy is required. Heating, high energy consumption, complex operation, long time crystallization leads to long production time of single batch, low efficiency, low water usage rate, large amount of waste water

The alkali extraction and acid precipitation method is based on the principle that naringin is easy to open the ring under alkaline conditions, and the solubility becomes larger, and it is closed under acidic conditions, and the solubility becomes smaller and precipitates, because naringin does not dissolve under hot alkaline conditions. It is stable and easy to decompose. If it is extracted at room temperature, the amount of water required is relatively large, and the raw material is under weakly alkaline conditions, due to the dissolution of low-ester pectin, gelation occurs, which affects the fluidity of the solution. , mainly found in laboratories, rare in industrial production

The organic solvent extraction method mainly uses ethanol heat reflux extraction, and ethanol extraction is used. Due to the excellent solubility of ethanol, a large amount of pigment, pectin, and other types of active ingredients in the raw material are dissolved, so it needs to be removed by water precipitation. Degreasing with non-toxic solvents, decolorizing with activated carbon, and multiple recrystallizations can obtain high-content naringin products. In addition, in the process of concentrating and recovering ethanol during production, the loss of ethanol is serious, the extraction cost is high, the process operation is complicated, and the time is long

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