A method for analyzing residual veterinary drugs in mutton

Abstract

The invention relates to the field of analysis and testing and particularly relates to a method for analyzing residual veterinary drugs in mutton. The method at least comprises sample pretreatment, enzymatic hydrolysis, extraction, purification and on-machine testing. Compared with the existing detection method, the method provided by the invention has higher accuracy, extracts, purifies and testsa variety of veterinary drugs, and is fast and efficient. The method utilizes a multi-extraction method using three different solvents, fully extracts the veterinary drugs in mutton as many as possible, improves the detection accuracy and reduces the influence caused by the base on the veterinary drugs. In gradient elution, three mobile phases are used so that it is solved that the original two mobile phases with solubility under action of an adsorbent in a chromatographic column are layered so that the target drugs are respectively dissolved in two solvents, the characteristic peak detectedby the detector splits into a primary characteristic peak and a secondary characteristic peak, the drug content capable of being detected is reduced and the determination of other drugs is influenced.

Description

technical field [0001] The invention relates to the field of detection and analysis of veterinary drugs, in particular to a method for analyzing residual veterinary drugs in mutton. Background technique [0002] Nowadays, the use of veterinary drugs is inevitable in animal husbandry production. In order to ensure food safety, my country has established a corresponding withdrawal period system for veterinary drugs. Withdrawal period for veterinary drug residues refers to the interval between the cessation of dosing of livestock and poultry and the approval of slaughter or the approval of their products on the market. Announcement No. 278 of the Ministry of Agriculture of the People's Republic of my country stipulates that all drugs or other chemical substances used for food animals must have a drug withdrawal period. However, in the actual animal husbandry process, due to the lack of relevant knowledge and the drive of economic interests, some livestock farmers failed to imp...

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Problems solved by technology

In addition, streptomycin can damage the baby's auditory nerve, causing hearing impairment in newborns

Certain benzimidazole veterinary drugs may cause mutations in human cells

Third, due to the long-term exposure of animals to antibacterial drugs, they accumulate in the body, and the drug-resistant microorganisms in the animal body are selectively multiplied, which reduces the efficacy of some commonly used drugs, such as penicillin, chloramphenicol, and gentamicin. , sulfonamides and other drugs have developed resistance in livestock production

In order to prohibit the excessive use of veterinary drugs in animal husbandry, many countries and international organizations, including China, have set strict limits on the residues of veterinary drugs in meat such as cattle, sheep, pigs, and horses, including "tentative maximum residues". ", "All limited standards", "Exempted eggs", etc., but there are still many problems in the detection of these veterinary drugs

[0004] The current veterinary drug detection methods in my country are mainly based on the classification of veterinary drugs. These methods have exposed several defects: for example, the screening ability for non-testing items of veterinary drug residues is insufficient, and for different types of veterinary drug residues, its detection method There are differences, so that the detection of veterinary drug residues can only measure the specified types of veterinary drugs, but lacks the ability to screen for other types of non-detected veterinary drug residues in the sample, which makes food safety monitoring hidden dangers

Secondly, different pretreatment and detection methods are required for different veterinary drug residue detection items of the same sample, which not only prolongs the detection cycle, increases detection cost and difficulty, but also reduces work efficiency

Furthermore, the extraction and purification process of traditional veterinary drug detection methods is cumbersome, costly, and time-consuming, which is difficult to meet the purpose of large-scale sample determination

Moreover, when detecting the content of residual veterinary drugs in meat, due to the relatively strong interaction between some veterinary drugs and the meat matrix, the detection process has a greater impact on the detection of some veterinary drugs, resulting in deviations in the detection limit and quantitative limit of these drugs. High, for example, salbutamol in mutton, due to its special structure, is affected by the mutton matrix and solvent during the detection process, the detected result is lower than its actual content, and may affect the test results of other veterinary drugs

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