Tomato long non-coding RNA-lncrna23468 and its cloning method and application method

A technology of long-chain non-coding and application methods, which is applied in the cloning and application field of tomato long-chain non-coding RNA-lncRNA23468, which can solve the problems of drug resistance of environmental pollution pathogens and problems of agricultural production, and achieve enhanced resistance, excellent effect, The effect of decreased expression
CN108085318BActive Publication Date: 2021-05-18DALIAN UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
DALIAN UNIV OF TECH
Publication Date
2021-05-18

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Abstract

The present invention provides a long-chain non-coding RNA-lncRNA23468 that improves resistance to tomato late blight by silencing miRNA482b, its DNA molecular sequence is shown in SEQ ID NO.1; a cloning method for the long-chain non-coding RNA is provided: Using the cDNA of wild-type late blight-resistant tomato L3708 as a template for PCR amplification, the obtained PCR product was connected to the pMD-19T cloning vector, transformed into Escherichia coli DH5α, and a single colony was picked for sequencing; the long-chain non-coding Methods of RNA application. After the long-chain non-coding RNA obtained by the method of the present invention is transiently overexpressed in tomato, the expression level of tomato miR482b is significantly reduced, and the resistance to late blight is significantly improved; it is used for silencing miR482b, which is important for cultivating tomato varieties resistant to late blight significance.
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Description

technical field

[0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to the cloning and application of a tomato long-chain non-coding RNA-lncRNA23468. Background technique

[0002] Long noncoding RNAs (lncRNAs) are a class of RNAs with a length of more than 200nt that do not contain a coding sequence (CDS), which are abundant in plants and have various biological functions. In recent years, with the rapid development of omics technology, lncRNAs have been identified in Arabidopsis, corn, rice, wheat, cotton, tomato, cucumber, poplar and other plants. Studies have found that plant lncRNAs play an important regulatory role in flowering regulation, photomorphogenesis and other developmental processes, as well as biotic and abiotic stress responses. During the interaction between plants and pathogens, four wheat lncRNAs respond to the infection of stripe rust; some Arabidopsis lncRNAs are induced by Fusarium fungus; some tom...

Claims

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