Check patentability & draft patents in minutes with Patsnap Eureka AI!

Bone tissue engineering scaffold capable of promoting rapid generation of blood vessels

A technology of bone tissue engineering and blood vessels, applied in the field of composite scaffolds and their preparation, to achieve the effects of improving adhesion, ensuring biological activity, and promoting angiogenesis

Inactive Publication Date: 2018-06-15
CHONGQING MEDICAL UNIVERSITY
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the preparation of multiple growth factor carrier systems using supercritical fluid technology for composite microspheres and scaffolds has not yet been reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bone tissue engineering scaffold capable of promoting rapid generation of blood vessels
  • Bone tissue engineering scaffold capable of promoting rapid generation of blood vessels
  • Bone tissue engineering scaffold capable of promoting rapid generation of blood vessels

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] Example 1 Preparation of PDGF-PLLA drug-loaded microspheres

[0023] 0.2 mg of PDGF was dissolved in 2.5 mL of dimethyl sulfoxide, and 200 mg of PLLA with a molecular weight of 10 ku was dissolved in 40 mL of dichloromethane. When both solutions are completely dissolved, mix them well and set aside. CO in the cylinder 2 After being liquefied by the refrigeration system, it is pressurized by a high-pressure plunger pump, heated by a constant temperature water bath in the pipeline, and pumped into a 500mL autoclave through the outer channel of the coaxial two-flow nozzle on the top of the autoclave. After the pressure in the kettle reaches 12Mpa, maintain CO 2 Pumping rate, open the vent valve to vent at a flow rate of 300ml / min, and keep the pressure in the kettle constant. Adjust the temperature of the external drying oven and pipeline water bath of the autoclave to keep the temperature inside the autoclave constant at 33°C. Then the mixed test solution was pumped i...

example 2

[0024] Preparation of Example Two Composite Support

[0025] PLGA (PLA:PLG=50:50, MW=ku) and HA powder were ground at a ratio of 1:4, and the grinding time was 8 hours. Add 20% PDGF-PLLA drug-loaded microspheres, and 20 μg FGF-2 and VEGF freeze-dried powder. FGF-2 and VEGF lyophilized powder were first centrifuged at 10 000r / min or 12 000r / min for 30s, resuspended in pH 7.6, 5mmol / L Tris to 0.1g / L, and added PLGA, HA and PDGF- PLLA microsphere powder was mixed gently and freeze-dried. The mixed powder was put into a cylindrical mold with a diameter of 5 mm and pressed at room temperature to obtain small discs, which were then taken out. Then place in a polytetrafluoroethylene cylinder mold and place the mold in supercritical CO 2 Stainless steel reactor. After the reactor is sealed, use a liquid pump to condense the CO condensed below 0°C 2 Pressed into the reactor, the temperature starts to rise when the reaction pressure is 8MPa, and the reaction temperature is 35°C. Th...

example 3

[0026] Induction of Example 3 Composite Scaffold to Angiogenesis in Chicken Embryo Allantoic Choriomembrane

[0027] Choose white-skinned eggs with clean surface and homogeneous shell, 50-65g per egg, standardized shape, uniform air chamber and pores. Rinse twice with warm water, then soak with bromogeramine lotion for 3 minutes. The chicken embryos were incubated in an incubator at a temperature of 37.8±0.5°C and a humidity of 40-60%. During the incubation process, the egg should be turned continuously to prevent the embryo from sticking and promote the movement of the amniotic membrane. The chick embryo is upright, at 45°. Tilt, turn eggs at least 2-4 times a day. Check the embryonic development status with the egg detection light every day, identify the symbolic characteristics of embryonic development, and eliminate stillbirths at any time. The second to sixth day of incubation is a critical period for the development of chicken embryos. After that, the embryos themsel...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention relates to a composite scaffold capable of promoting rapid generation of blood vessels in bone tissue engineering and a preparation method thereof. In the invention, PLLA (levorotatory polylactic acid) microspheres loaded with PDGF (platelet derived growth factor) are prepared by adopting a supercritical fluid forced dispersion solution technology; then a supercritical carbon dioxidefoaming technology is used to load the microspheres, a VEGF (vascular endothelial growth factor) and an FGF-2 (basic fibroblast growth factor) into PLGA (polylactic acid-glycolic acid) porous scaffold together; and finally, vascular endothelial cells are planted on the scaffold to construct a composite scaffold which can cooperatively regulate and control the generation of blood vessels. In the invention, the planted vascular endothelial cells can differentiate into new blood vessels, the extracellular matrix secreted by the planted vascular endothelial cells can improve the adhesion of related cells, the secondary release of three vascular induction factors can induce the growth of blood vessels, and the synergistic effect of the three can promote the rapid generation of blood vessels ina bone repair process. The preparation of the scaffold provided by the invention is simple in operation, and the prepared composite scaffold has good biocompatibility and functionality, and has an application prospect in tissue engineering restorations.

Description

technical field [0001] The invention relates to a composite scaffold capable of promoting the rapid formation of blood vessels in bone tissue engineering and its preparation, and belongs to the technical field of drug dosage form change and its preparation method. Specifically, it is a new method of using supercritical fluid technology to combine microspheres and scaffolds to prepare vascular tissue engineering scaffolds, especially involving supercritical fluid forced dispersion solution technology and supercritical foaming technology for drug-loaded microspheres and composite scaffolds. The green controllable preparation process and the sequential controllable release of three vascular induction factors including PDGF, FGF-2 and VEGF in the composite scaffold. Background technique [0002] Blood vessels are not only transport channels for oxygen, various nutrients, and metabolic wastes, but also an important pathway for cell signaling molecules involved in tissue repair to...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L27/12A61L27/54A61L27/38
CPCA61L27/12A61L27/3808A61L27/3847A61L27/54A61L2300/252A61L2300/414A61L2300/602
Inventor 白燕丁庆
Owner CHONGQING MEDICAL UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com