Use of dietary fiber extract from oudemansiellara maflbes in preparation of preparations for treating and/or preventing liver injury-related diseases caused by high fat diet

A technology of dietary fiber and Odermushroom, which is applied in health care products. In the field of pharmaceuticals, the extract of dietary fiber of Ouddermis The effect of rate and purity

Inactive Publication Date: 2018-08-10
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is the extract of Oudemansiellara Maflbes, Oudemansiellara Maflbes, which is an edible fungus with great nutritional, health care and medicinal value, and there are few studies on it at present

Method used

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  • Use of dietary fiber extract from oudemansiellara maflbes in preparation of preparations for treating and/or preventing liver injury-related diseases caused by high fat diet
  • Use of dietary fiber extract from oudemansiellara maflbes in preparation of preparations for treating and/or preventing liver injury-related diseases caused by high fat diet
  • Use of dietary fiber extract from oudemansiellara maflbes in preparation of preparations for treating and/or preventing liver injury-related diseases caused by high fat diet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation method of the dietary fiber extract of Ouderia oosporus:

[0046] (1) After the sample is dried, it is pulverized and weighed. The ratio of solid to liquid is 1:10. It is boiled and refluxed with 95% ethanol to extract for 2 hours, filtered with suction, and the filter residue is retained;

[0047] (2) add water to the filter residue, the ratio of solid to liquid is 1:10, boil and reflux for extraction for 2 hours;

[0048] (3) When the temperature drops to about 60°C, adjust the pH value to 6-7, add papain for enzymatic hydrolysis, the enzyme amount is 0.2% of the weighed sample, enzymatically hydrolyze in a water bath at 60°C for 1 hour, and keep stirring during the period;

[0049] (4) After the enzymatic hydrolysis is completed, add 95% ethanol with 4 times the volume of the extract to precipitate overnight, filter with suction, and retain the filter residue;

[0050] (5) The filter residue is gradient eluted with 78% ethanol, 95% ethanol, absolute et...

Embodiment 2

[0056] Example 2: Improving effect of Ouderia oodota dietary fiber extract on liver injury in obese mice induced by high-fat diet

[0057] 1. Reagents:

[0058] Total cholesterol (T-CHO) test kit: Nanjing Jiancheng Institute of Bioengineering.

[0059] Triglyceride (TG) test kit: Nanjing Jiancheng Institute of Bioengineering.

[0060] Low-density lipoprotein cholesterol (LDL-C) test kit: Nanjing Jiancheng Bioengineering Institute.

[0061] High-density lipoprotein cholesterol (HDL-C) test kit: Nanjing Jiancheng Bioengineering Institute.

[0062] Alanine Aminotransferase (ALT) Test Kit, Aspartate Aminotransferase (AST) Test Kit, Total Protein (TP) Test, Albumin (ALB) Test and Total Bilirubin (T-Bil) Test Kit All were purchased from Nanjing Jiancheng Institute of Bioengineering.

[0063] Thirty female C57 mice, SPF grade, weighing 12-14 g, and 28-30 days old, were purchased from Guangdong Medical Experimental Animal Center with license number SCXK (Guangdong) 2013-0002.

[...

Embodiment 3

[0101] Example 3: The improvement effect of the dietary fiber extract of Ouderia oosporus on the diseases related to intestinal flora imbalance in obese mice induced by high-fat diet

[0102] 1. Experimental method

[0103] The feces of the mice at the end of the animal experiment in Example 2 were collected, quickly frozen with liquid nitrogen, and stored in a -80°C refrigerator. DNA was extracted from stool samples, and the quality of DNA extraction was detected by 0.8% agarose gel electrophoresis, and DNA was quantified by UV spectrophotometer. Forward primer 5'-ACTCCTACGGGAGGCAGCA-3' and reverse primer 5'-GGACTACHVGGGTWTCTAAT-3' were used to amplify the V3-V4 region gene of mouse 16S rRNA. The PCR amplification products were detected by 2% agarose gel electrophoresis, and the target fragments were recovered by cutting the gel, and the recovery was performed using the gel recovery kit of AXYGEN Company. Referring to the preliminary quantitative results of electrophoresis,...

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Abstract

The invention relates to use of a dietary fiber extract from oudemansiellara maflbes in the field of pharmacy, in particular to use of a dietary fiber extract from the oudemansiellara maflbes in preparation of preparations for treating and/or preventing liver injury-related diseases caused by high fat diet. The dietary fiber extract from the oudemansiellara maflbes not only can significantly contribute to the repair of liver injury caused by the high fat diet, but also shows obvious therapeutic and/or preventive effects on the liver injury-related diseases; for example, the dietary fiber extract from the oudemansiellara maflbes effectively inhibits the expression of inflammatory factors, effectively alleviates the deterioration of inflammation, improves various physiological and pathological indicators related to immunity, and can be used in various fields such as medicines and health care products, thus having great economic and social values.

Description

technical field [0001] The present invention relates to the use of the dietary fiber extract of Ouderia oosporus in the fields of pharmacy, health care products, etc., and particularly relates to the use of the dietary fiber extract of Ouderia oodactana in the preparation of treatment and / or prevention of liver damage caused by high-fat diet. Use in the preparation of diseases. Background technique [0002] Obesity refers to the excessive accumulation of fat in the human body, which obviously exceeds the average amount of normal people, and there are abnormal changes in body metabolism, physiology and biochemistry, which is called obesity. As of 2014, 600 million people in the world are obese. It is estimated that by 2025, the number of obese people in the world will reach 1.4 billion. Obesity has become one of the public health problems that attract worldwide attention. Studies have found that the occurrence and development of common diseases in wealthy societies are mostl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715C08B37/00A23L33/21A61P1/16A61K36/07
CPCA23L33/21A23V2002/00A61K31/715A61K36/07A61P1/16C08B37/0003A23V2200/32A23V2250/208
Inventor 陈地灵杨俭唐晓萃帅欧赖国校谢意珍吴清平杨柏华
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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