Composite microbial preparation for aquaculture and method
A technology for compound microorganisms and aquaculture, which is applied in the field of compound microorganism preparations for aquaculture, which can solve the problems of loss of manpower and material resources, low survival rate, overpowering, etc., and achieve the effect of increasing production, reducing disease rate and mortality
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preparation example Construction
[0028] The steps of the above-mentioned compound microbial preparation preparation method are as follows:
[0029] Fermentation: Mix the strains of photosynthetic bacteria, Bacillus subtilis, lactic acid bacteria, nitrifying bacteria, Bdellovibrio and yeast with water in a mass ratio of 1:10, and carry out single-strain sealed fermentation in the culture medium to obtain the fermentation liquid; the culture medium The pH is controlled at 5-7, the culture temperature is controlled at 25-35°C, and the culture time is subject to a pH of 3-6 at the end of fermentation;
[0030] The medium used for fermentation is composed of 15% amino acid, 75% peptone, 8% inorganic salt and 2% trace elements by mass ratio; the inorganic salt is composed of 6% sodium chloride, 1.8% hydrogen phosphate Dipotassium and 0.2% potassium dihydrogen phosphate; trace elements are composed of 0.8% calcium, 0.6% iron and 0.6% zinc in mass ratio;
[0031] Bacteria powder preparation: Centrifuge and concentra...
Embodiment 1
[0043] Embodiment 1: splash in the pond of cultured shrimp (the water in the pond of choosing is 100m )
[0044] The components of the spilled composite microbial preparation are as follows according to the mass ratio:
[0045] Photosynthetic bacteria powder 0.8%, Bacillus subtilis powder 1%, Lactic acid bacteria powder 1%
[0046] Nitrifying bacteria powder 1%, Bdellovibrio powder 1.5%, Yeast powder 0.5%
[0047] Multivitamin 1.6%, the balance is carrier;
[0048] Among them, the carrier is corncob; the multivitamin is composed of 0.2% vitamin A, 0.3% vitamin B1, 0.3% vitamin B2, 0.3% vitamin B6, 0.3% vitamin B12, and 0.2% vitamin C;
[0049] Composite microbial preparations contain 5 billion viable bacteria / g, moisture ≤ 2%;
[0050] Fermentation: the strains of photosynthetic bacteria, Bacillus subtilis, lactic acid bacteria, Bdellovibrio, nitrifying bacteria and saccharomyces were respectively mixed with water at a mass ratio of 1:10 to carry out single-strain sealed fe...
Embodiment 2
[0056] Embodiment 2: splash in the pond of culture crab (the water in the pond of choosing is 150m )
[0057] The components of the spilled composite microbial preparation are as follows according to the mass ratio:
[0058] Photosynthetic bacteria powder 2%, Bacillus subtilis powder 3%, Lactic acid bacteria powder 1%
[0059] Nitrifying bacteria powder 1.5%, Bdellovibrio powder 4.8%, Yeast powder 1%
[0060] Multivitamin 2.25%, the rest is carrier;
[0061] Wherein, the carrier is corn starch; the multivitamin is composed of 0.3% vitamin A, 0.4% vitamin B1, 0.4% vitamin B2, 0.4% vitamin B6, 0.4% vitamin B12, and 0.35% vitamin C;
[0062] Composite microbial preparations contain 5 billion viable bacteria / g, moisture ≤ 2%;
[0063] The steps of the compound microbial preparation preparation method are as follows:
[0064] Fermentation: the strains of photosynthetic bacteria, Bacillus subtilis, lactic acid bacteria, Bdellovibrio, nitrifying bacteria and saccharomyces were re...
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