Composite microbial preparation for aquaculture and method

A technology for compound microorganisms and aquaculture, which is applied in the field of compound microorganism preparations for aquaculture, which can solve the problems of loss of manpower and material resources, low survival rate, overpowering, etc., and achieve the effect of increasing production, reducing disease rate and mortality

Inactive Publication Date: 2018-10-16
安徽在天生态农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The common problems in the aquaculture industry are: the alkalinity of the aquaculture water is too high or too strong, the content of ammonia nitrogen and nitrite is high; due to the existence of the aforementioned harmful substances in the water body, there are often algal blooms in the aquaculture ponds. , resulting in excessive water color, blackening, redness, turbidity and other aging phenomena, which in turn promotes the breeding of a variety of toxic pathogenic bacteria in the water body, such as Aeromonas hydrophila, Vibrio, Escherichia coli, Streptococcus, etc.; thus causing br

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] The steps of the above-mentioned compound microbial preparation preparation method are as follows:

[0029] Fermentation: Mix the strains of photosynthetic bacteria, Bacillus subtilis, lactic acid bacteria, nitrifying bacteria, Bdellovibrio and yeast with water in a mass ratio of 1:10, and carry out single-strain sealed fermentation in the culture medium to obtain the fermentation liquid; the culture medium The pH is controlled at 5-7, the culture temperature is controlled at 25-35°C, and the culture time is subject to a pH of 3-6 at the end of fermentation;

[0030] The medium used for fermentation is composed of 15% amino acid, 75% peptone, 8% inorganic salt and 2% trace elements by mass ratio; the inorganic salt is composed of 6% sodium chloride, 1.8% hydrogen phosphate Dipotassium and 0.2% potassium dihydrogen phosphate; trace elements are composed of 0.8% calcium, 0.6% iron and 0.6% zinc in mass ratio;

[0031] Bacteria powder preparation: Centrifuge and concentra...

Embodiment 1

[0043] Embodiment 1: splash in the pond of cultured shrimp (the water in the pond of choosing is 100m )

[0044] The components of the spilled composite microbial preparation are as follows according to the mass ratio:

[0045] Photosynthetic bacteria powder 0.8%, Bacillus subtilis powder 1%, Lactic acid bacteria powder 1%

[0046] Nitrifying bacteria powder 1%, Bdellovibrio powder 1.5%, Yeast powder 0.5%

[0047] Multivitamin 1.6%, the balance is carrier;

[0048] Among them, the carrier is corncob; the multivitamin is composed of 0.2% vitamin A, 0.3% vitamin B1, 0.3% vitamin B2, 0.3% vitamin B6, 0.3% vitamin B12, and 0.2% vitamin C;

[0049] Composite microbial preparations contain 5 billion viable bacteria / g, moisture ≤ 2%;

[0050] Fermentation: the strains of photosynthetic bacteria, Bacillus subtilis, lactic acid bacteria, Bdellovibrio, nitrifying bacteria and saccharomyces were respectively mixed with water at a mass ratio of 1:10 to carry out single-strain sealed fe...

Embodiment 2

[0056] Embodiment 2: splash in the pond of culture crab (the water in the pond of choosing is 150m )

[0057] The components of the spilled composite microbial preparation are as follows according to the mass ratio:

[0058] Photosynthetic bacteria powder 2%, Bacillus subtilis powder 3%, Lactic acid bacteria powder 1%

[0059] Nitrifying bacteria powder 1.5%, Bdellovibrio powder 4.8%, Yeast powder 1%

[0060] Multivitamin 2.25%, the rest is carrier;

[0061] Wherein, the carrier is corn starch; the multivitamin is composed of 0.3% vitamin A, 0.4% vitamin B1, 0.4% vitamin B2, 0.4% vitamin B6, 0.4% vitamin B12, and 0.35% vitamin C;

[0062] Composite microbial preparations contain 5 billion viable bacteria / g, moisture ≤ 2%;

[0063] The steps of the compound microbial preparation preparation method are as follows:

[0064] Fermentation: the strains of photosynthetic bacteria, Bacillus subtilis, lactic acid bacteria, Bdellovibrio, nitrifying bacteria and saccharomyces were re...

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Abstract

The invention discloses a composite microbial preparation for aquaculture and method. The composite microbial preparation comprises the compositions of, by mass, 0.8-2% of photosynthetic bacteria powder, 1-3% of bacillus subtilis powder, 0.5-1% of lactic acid bacteria powder, 0.5-1.5% of nitrifying bacteria powder, 1.5-4.8% of bdellovibrio bacteria powder, 0.5-1% of saccharomycete bacteria powder,1.6-2.25% of multi-vitamins and 84.45%-93.6% of carriers. The preparation method comprises the three steps of fermentation, bacteria powder preparation and preparing of the preparation. The compositemicrobial preparation can be used after amplification cultureor directly sprayed. When the composite microbial preparation is applied to the aquaculture industry, nitrite, ammonia nitrogen and virosin which are in water are decomposed by strains symbiosis and enzymatic hydrolysis, so that the effects of purifying water quality and lowering pH are achieved, the incidence rate of cultured organismscan be greatly reduced, the income of aquaculturists is improved, and green and healthy aquaculture is achieved.

Description

technical field [0001] The invention relates to a compound microbial preparation and method, in particular to a compound microbial preparation and method for aquaculture. Background technique [0002] Composite microbial preparations are microbial preparations made from two or more beneficial microbial strains that are not antagonistic to each other. Beneficial bacteria use their own physiological metabolism to process organic matter in water, decompose ammonia nitrogen, nitrite, hydrogen sulfide and other adverse factors in water, purify water quality, solve water quality problems caused by various toxins, reduce the risk of breeding animals, and effectively inhibit breeding The occurrence of animal symptoms reduces the need for antibiotics in farmed animals, and the interaction of beneficial bacteria in compound biological preparations is used to achieve healthy farming. In addition, after the action of beneficial bacteria, a large number of good beneficial bacteria group...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N11/02C12N11/10C02F3/34C02F101/16C12R1/01C12R1/125
CPCC12N1/20A01K61/13A23K10/12A23K10/18A23K10/20A23K10/33A23K10/37A23K20/163A23K20/174A23K50/80C02F3/34C02F3/347C02F2101/16C02F2101/166C02F2209/06C12N1/16C12N11/02C12N11/10
Inventor 程英
Owner 安徽在天生态农业开发有限公司
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