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Preparation method of pigskin antibacterial polypeptide

The technology of antibacterial polypeptide and antibacterial peptide is applied in the field of preparation of pigskin antibacterial polypeptide, which can solve the problem of little extraction, and achieve the effects of removing fishy smell substances, high yield and purity, and high degreasing rate.

Inactive Publication Date: 2018-10-26
金华市铁骑士生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the antimicrobial peptides from pigs are mainly related to the antimicrobial peptides in the small intestine of pigs in domestic and foreign research, but there are few studies on the extraction and purification of antimicrobial peptides from pig skin and their antibacterial activity.

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  • Preparation method of pigskin antibacterial polypeptide

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Embodiment 1

[0016] A method for preparing pigskin antibacterial polypeptides, comprising raw material pretreatment, fermentation medium preparation, Aspergillus niger fermentation, ultrafiltration, and purification, the specific steps of which are:

[0017] 1) Raw material pretreatment: cut fresh pigskin into pieces, add 3% sodium carbonate and 0.031% ethyl valerate aqueous solution at a material-liquid ratio of 1:8 (g / mL), and heat it at a temperature of 45°C and an ultrasonic frequency Degreasing under the condition of 40kHz for 60 minutes, discarding the lye, washing with water until nearly neutral, then adding water to homogenate according to the ratio of material to liquid at 1:8 (g / mL), heating and boiling, filtering, and drying to obtain degreased pork skin. Under the assisted action of ultrasonic waves, the grease can be quickly formed into water-soluble soap salt and glycerin to achieve the purpose of degreasing, and the degreasing rate is high. On the one hand, the addition of et...

Embodiment 2

[0024] A method for preparing pigskin antibacterial polypeptides, comprising raw material pretreatment, fermentation medium preparation, Aspergillus niger fermentation, ultrafiltration, and purification, the specific steps of which are:

[0025] 1) Raw material pretreatment: cut fresh pigskin into pieces, add 2% sodium carbonate and 0.035% ethyl valerate aqueous solution at a material-to-liquid ratio of 1:10 (g / mL), at a temperature of 40°C and an ultrasonic frequency Degrease under the condition of 45kHz for 45min, discard the lye, wash with water until nearly neutral, then add water to homogenate according to the material-liquid ratio of 1:10 (g / mL), heat to boil, filter, and dry to obtain defatted pork skin;

[0026] 2) Fermentation medium preparation: Take 10 parts of defatted pork skin, 20 parts of sucrose, 5 parts of sodium chloride, 0.005 parts of zinc chloride, 2.0 parts of potassium dihydrogen phosphate, 0.4 parts of magnesium sulfate, and 0.01 parts of iron sulfate by...

Embodiment 3

[0032] A method for preparing pigskin antibacterial polypeptides, comprising raw material pretreatment, fermentation medium preparation, Aspergillus niger fermentation, ultrafiltration, and purification, the specific steps of which are:

[0033] 1) Raw material pretreatment: cut fresh pigskin into pieces, add 2% sodium carbonate aqueous solution according to the material-liquid ratio of 1:10 (g / mL), and degrease for 45 minutes at a temperature of 40°C and an ultrasonic frequency of 45kHz , discard the lye, wash with water until nearly neutral, then add water to homogenate according to the material-to-liquid ratio of 1:10 (g / mL), heat to boil, filter, and dry to obtain defatted pork skin;

[0034] 2) Fermentation medium preparation: Take 10 parts of defatted pork skin, 20 parts of sucrose, 5 parts of sodium chloride, 0.005 parts of zinc chloride, 2.0 parts of potassium dihydrogen phosphate, 0.4 parts of magnesium sulfate, and 0.01 parts of iron sulfate by weight , Vitamin B 1 ...

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Abstract

The invention discloses a preparation method of pigskin antibacterial polypeptide. The method specifically comprises the steps that fresh pigskin is cut and put into an aqueous solution containing sodium carbonate and ethyl valerate, then degreasing is carried out, an alkali solution is discarded, washing is carried out until the solution is near neutral, then water is added for homogenate, boiling, filtering and drying are carried out to obtain degreased pigskin; the degreased pigskin is utilized to prepare a fermentation medium, aspergillus niger is inoculated for fermentation, and liquid supernatant is centrifuged to obtain a fermentation broth; the fermentation broth is ultra-filtered to obtain a crude antibacterial peptide extract; the crude antibacterial peptide extract is subjectedto gel chromatography, after sample loading is carried out, elution is carried out by using double distilled water, components with higher antibacterial activity are collected, and freeze-dried to obtain the pigskin antibacterial peptide. The preparation method of the pigskin antibacterial polypeptide has the advantages that the extraction method is simple and feasible, the extraction efficiency,the yield and the purity are high, and the prepared antimicrobial peptide has high quality and high activity, can quickly kill bacteria, and has broad-spectrum antibacterial properties.

Description

technical field [0001] The invention relates to the technical field of biological extraction, in particular to a preparation method of pigskin antibacterial polypeptide. Background technique [0002] Since the discovery of antibiotics, it has been widely used in medical treatment, traditional veterinary medicine and feed additives due to its good antibacterial effect. The more serious it is, it has gradually attracted people's attention. It is extremely urgent to find effective alternatives to antibiotics and solve the problems of drug resistance and drug residues. Antimicrobial peptides (AMPs) are widely distributed and diverse polypeptide substances with broad-spectrum antibacterial activity that can be produced by both prokaryotes and eukaryotes. Negative bacteria, fungi, protozoa, cancer cells, viruses, etc. all have obvious inhibitory effects, and are regarded as a substitute for antibiotics with great application prospects, and have become a research hotspot at home a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C07K1/34C12R1/685
CPCC12P21/00C07K1/34
Inventor 全盈园
Owner 金华市铁骑士生物科技有限公司
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