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A kind of molecularly imprinted amphiphilic chitosan derivative lb membrane modified electrode and its construction method and application

A chitosan derivative and molecular imprinting technology is applied in the analysis of materials, material electrochemical variables, material analysis by electromagnetic means, etc., to achieve the effects of controllable film thickness, fast electrochemical response speed, and high recognition

Inactive Publication Date: 2020-08-07
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No work has been reported so far on imprinting with non-liposomal amphiphiles

Method used

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  • A kind of molecularly imprinted amphiphilic chitosan derivative lb membrane modified electrode and its construction method and application
  • A kind of molecularly imprinted amphiphilic chitosan derivative lb membrane modified electrode and its construction method and application
  • A kind of molecularly imprinted amphiphilic chitosan derivative lb membrane modified electrode and its construction method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Synthesis of Amphiphilic Cholesterol-Chitosan Carbonate. The steps are as follows: first in the reaction vessel, get 5.49 g cholesterol chloroformate and dissolve in N, N - In dimethylformamide, in the presence of 2.4 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride with stirring, react for 5 h (50°C) to obtain activated cholesterol chloride Formate solution; then add 1.0 g chitosan and methanesulfonic acid in another container, stir until chitosan is completely dissolved, under nitrogen protection, add the activated cholesterol chloroformate solution obtained in the above steps dropwise, React at 50°C for 60 h. The product mixed system is precipitated, centrifuged, washed and freeze-dried to obtain cholesterol chitosan carbonate solid. The chemical structure of the product was confirmed by infrared spectrum and carbon nuclear magnetic spectrum.

Embodiment 2

[0047] (1) Preparation of Amphiphilic Cholesterol-Chitosan Carbonate and Histamine Solution

[0048] The cholesterol-chitosan carbonate solid prepared in Example 1 was formulated into a chloroform solution of cholesterol-chitosan carbonate 1.00 mg / mL for subsequent use; histamine was dissolved in ultrapure water to prepare a concentration of 1.0 mg / mL Amine solution, stored under low temperature conditions, for later use;

[0049] (2) Formation of Langmuir film of cholesterol-chitosan carbonate / histamine mixed component

[0050] After completing the preliminary work according to the requirements of the operation method of the Langmuir tank, add ultrapure water (resistivity ≥

[0051] 18.25 Ω m) as a subphase, add 100 μL of histamine aqueous solution with a concentration of 0.2 mg / mL [diluted from the histamine aqueous solution prepared in step (1) of Example 2] dropwise with a micro-injector, and mix well Then use a microsampler to pipette 25 μL of the cholesterol-chitosan c...

Embodiment 3

[0053] Formation of Langmuir films of cholesterol-chitosan carbonate / histamine mixture components. After completing the preliminary work according to the requirements of the Langmuir tank operation method, add ultrapure water (resistivity ≥ 18.25 Ω m) into the tank as a subphase, and add 100 μL of histamine with a concentration of 0.2 mg / mL dropwise with a micro-injector Aqueous solution [formed by diluting the histamine aqueous solution prepared in step (1) of Example 2], after mixing evenly, pipette 50 μL of cholesterol-chitosan carbonate prepared in step (1) of Example 2 with a micro-sampler Chloroform solution, slowly added dropwise to the surface of the subphase water, making it spread on the water surface. After the chloroform is completely volatilized, the pressure sensor is reset to zero, and the sliding speed is fixed at 50 cm 2 / min, start to compress the sliding barriers A and B, and obtain a Langmuir film (monolayer) of cholesterol-chitosan carbonate / histamine mixed...

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Abstract

The invention discloses a molecular imprinting amphiphilic chitosan derivative LB film modified electrode, a construction method and application thereof. The method comprises the following steps: firstly performing reconstruction combination in an air / water interface through a Langmuir technology and interaction force of functional groups between amphiphilic cholesterol-chitosan carbonic ester andhistamine, so as to form a cholesterol-chitosan carbonic ester / histamine mixed component monomolecular film; transferring the mixed component monomolecular film onto an ITO glass electrode through aLB technology; then eluting histamine from a mixed component LB film through a molecular imprinting method, and forming a molecular imprinting LB film electrode for selectively recognizing histamine;finally performing measurement to find that the electrochemical responsiveness to histamine by the molecular imprinting LB film electrode is high, histamine is wide in linear detection range, low in detection limit, and excellent in selectivity, repeatability and stability, and trace histamine in beer and grape wine can be rapidly and sensitively detected through an electrochemical method. The method opens up a novel path for a surface molecular imprinting technology.

Description

technical field [0001] The invention belongs to the field of functional polymer ultra-thin films, and in particular relates to a molecularly imprinted amphiphilic chitosan derivative LB film-modified electrode and a construction method and application thereof. Background technique [0002] Histamine (HA), 2-(4-imidazole)-ethylamine, is produced in the human body by the decarboxylation of histidine through the catalysis of histidine decarboxylase (HDC), and is widely distributed and powerful in human tissue cells. One of the biologically active substances. Histamine participates in the regulation of various physiological functions in the body such as cardiovascular circulation and the pathological process of diseases such as tumors through the mediation of G protein-coupled receptors. With the help of modern genetic engineering technology, people have been able to explore the structure of histamine receptors and the interaction with histamine from the genetic level. So far,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/30G01N27/3278
Inventor 关怀民童跃进刘玉洁
Owner FUJIAN NORMAL UNIV
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