Wintercherry fermentation fruit juice and preparation method thereof
A technology of sour berry and fermented raw juice, applied in the direction of bacteria used in food preparation, functions of food ingredients, food ingredients containing natural extracts, etc., can solve unacceptable, low utilization rate of active ingredients, limitations of development and utilization, etc. problems, to achieve the effect of benefiting digestion and absorption, improving antioxidant performance, and broadening product categories
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Embodiment 1
[0052] The preparation of embodiment 1 sour berry original juice
[0053] Select sour berries that are free from insects, rot, and full of fruit, wash them three to four times under running water, remove the stems, and put them in the juicer. Use a juicer to crush the sour berries, and the crushing time is 1 to 2 minutes. Add 0.2% to 0.4% of pectinase and 0.1% to 0.3% cellulase in the juice of physalis respectively, stir evenly, and carry out enzymolysis in water bath at 35 to 45°C. The solution time is 30min~50min. After the enzymatic hydrolysis is completed, inactivate the enzyme in water at 90°C to 100°C for 10min to 15min. After the enzyme inactivation is completed, use gauze to filter, remove the fruit peel and seeds of the sour berry, and obtain the filtrate which is the original juice of the sour berry.
Embodiment 2
[0054] Activation, domestication and selection of embodiment 2 strains
[0055] (1) Activation method
[0056] Take the prepared MRS liquid medium, sterilize it and let it cool down, inoculate an appropriate amount of bacteria powder into the medium under aseptic conditions, mix well and place it in a 37°C constant temperature incubator for 24 hours. The alternative fermentation strains of sour pulp fermented juice are lactic acid bacteria: Lactobacillus plantarum (CICC20022), Lactobacillus acidophilus (CICC6006), Lactobacillus brevis (CICC6239), Lactobacillus rhamnosus (CICC20255) and Lactobacillus paracasei ( CICC6239) These strains are commercially available. The five lactic acid bacteria strains were subcultured for 3 to 4 times in the culture medium. When the viable count reaches 10 7 When the CFU / g is above, the strain can reach normal vigor, and it should be stored in the refrigerator for later use. When the bacterial strain is a dry powder, activation is required, ...
Embodiment 3
[0064] Determination of the proportion of fermented strains of sour berry original juice of embodiment 3
[0065] Since the fermentation effect of compound strains is better than that of single strains, the selected Lactobacillus plantarum and Lactobacillus acidophilus are compounded, and the compounding ratio is 1:1, 2:1, 1:2 according to the mass ratio. . Lactobacillus acidophilus (La) and Lactobacillus plantarum (Lp) were inoculated in the raw juice of sour berries according to the mass ratio of 1:1, 2:1, and 1:2, and cultured at 35°C to 38°C for 48 hours. Measure the number of viable bacteria, acidity, and pH value once every 4 hours, so as to select the appropriate addition ratio of the composite bacteria. According to the growth curves of different proportions of strains fermented for 48 hours, combined with lactic acid content, pH value and sensory evaluation, the final starter was determined, and the results were as follows Figure 4 , 5 , 6, and 7. according to ...
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