Electrochemiluminescence method for detecting carcinoembryonic antigen and kit thereof

A technology of electrochemistry and carcinoembryonic antigen, which is applied in the field of analytical chemistry and nanometers, can solve the problems of low quantum yield, few gold nanoclusters, and unclear luminescent mechanism

Inactive Publication Date: 2019-01-04
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limitations of its low electrochemiluminescence intensity, low quantum yield, and unclear lu...

Method used

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  • Electrochemiluminescence method for detecting carcinoembryonic antigen and kit thereof
  • Electrochemiluminescence method for detecting carcinoembryonic antigen and kit thereof
  • Electrochemiluminescence method for detecting carcinoembryonic antigen and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Add 0.6 mL of 0.5 mol / L NaOH and 0.4 mL of 20 mg / mL chloroauric acid solution into 4 mL of 0.08 mol / L N-acetyl-L-cysteine ​​solution, mix well and place at 37 °C for constant temperature Incubate for 3 hours in the sink. After the reaction was completed, the reaction solution was dialyzed and purified for 24 h with a dialysis bag with a molecular weight of 3000 to obtain the purified N-acetyl-L-cysteine-protected gold nanocluster solution, which was stored in a refrigerator at 4 °C in the dark.

Embodiment 2

[0050] Glassy carbon electrodes with a diameter of 3 mm were coated with 1.0 μm, 0.3 μm, 0.05 μm Al 2 o 3 The powder is polished and polished in turn until it reaches a smooth mirror surface, and then put in HNO in turn 3 solution, absolute ethanol, ultrasonic cleaning in deionized water for 3 minutes, N 2 blow dry. Take 5 μL of the N-acetyl-L-cysteine-protected gold nanocluster solution prepared in Example 1 and drop it on the surface of the treated glassy carbon electrode, dry it at room temperature, and further soak the electrode in 0.1 mol / L React in sodium borohydride solution for 5 minutes to obtain gold nanocluster probe modified electrode. Insert the above electrodes into 0.1 mol / L pH 7.4 phosphate buffer solution containing 0.1 mol / L potassium persulfate and 0.1 mol / L KCl. The step pulse method was adopted, the initial potential was 0 V, the pulse time was 10 s, the end potential was -2 V, and the pulse time was 1 s. The high voltage of the photomultiplie...

Embodiment 3

[0052] Glassy carbon electrodes with a diameter of 3 mm were coated with 1.0 μm, 0.3 μm, 0.05 μm Al 2 o 3 The powder is polished and polished in turn until it reaches a smooth mirror surface, and then put in HNO in turn 3 solution, absolute ethanol, ultrasonic cleaning in deionized water for 3 minutes, N 2 blow dry. Take 5 μL of the N-acetyl-L-cysteine-protected gold nanocluster solution prepared in Example 1 and drop it on the surface of the treated glassy carbon electrode, dry it at room temperature, and further soak the electrode in 0.1 mol / L react in sodium borohydride solution for 5 minutes to obtain a gold nanocluster probe modified glassy carbon electrode. A three-electrode system was adopted, with gold nanocluster probe-modified glassy carbon electrode as the working electrode, platinum wire electrode as the counter electrode, and Ag / AgCl as the reference electrode. 4 -H 2 SO 4 (1:1 V / V) solution, manganese dioxide was electrodeposited, the deposition poten...

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Abstract

The invention discloses an electrochemiluminescence method for detecting a carcinoembryonic antigen and a kit thereof. The method adopts the carcinoembryonic antigen as a detection object, organicallycombines a high quantum yield gold nanocluster electrochemiluminescence technology and an immunoassay technology, adopts a reduction method to prepare a high quantum yield gold nanocluster electrochemiluminescence probe, and uses a manganese dioxide nanomaterial as an electrochemiluminescence quencher to recover an electrochemiluminescence signal by an oxidation-reduction reaction of manganese dioxide and ascorbic acid produced by an enzyme-linked immunosorbent reaction, thereby developing the novel high-performance electrochemiluminescence carcinoembryonic antigen detection method based on high quantum yield gold nanocluster probes and the detection kit. The linear range of detection of a carcinoembryonic antigen in the method is 3*10-5 ng/mL-3 ng/mL, and the detection limit is 27 fg/mL.The method has the advantages of being fast, accurate, high in sensitivity, good in selectivity and stability, low in sample consumption and the like, and has a good clinical application prospect.

Description

technical field [0001] The invention relates to an electrochemiluminescent carcinoembryonic antigen detection method and a detection kit based on a high-quantum-yield gold nanocluster probe, belonging to the fields of analytical chemistry and nanotechnology. Background technique [0002] Malignant tumors are an important class of diseases that affect people's lives and health, and have become the largest public health problem in the world. The confirmation and sensitive detection of tumor markers have been widely used in the diagnosis of malignant tumors and the judgment of curative effect, recurrence and prognosis. Carcinoembryonic antigen is an internationally recognized tumor marker. Clinically, the detection of carcinoembryonic antigen is of great significance to the diagnosis of lung cancer, gastric cancer, colon cancer, pancreatic cancer and other glandular malignant tumors. At present, many immunoassay methods for detecting the content of carcinoembryonic antigen in ...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N27/416G01N33/53
CPCG01N21/76G01N27/416G01N33/53
Inventor 彭花萍黄种南陈伟吴伟华庄琼琼
Owner FUJIAN MEDICAL UNIV
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