Blood lead cleaner for blood lead cleaning in vitro
A technology of blood and blood lead, which is applied in the field of biomedical instruments, can solve problems such as inability to enter red blood cell lead ions, impossibility and poor removal of lead ions, and severe blood lead poisoning, so as to overcome unclear removal principles, relieve illness, compose simple effects
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[0041] Example 1
[0042] 1) Preparation of magnetic ferroferric oxide (Fe 3 O 4 NPs)
[0043] Weigh ferric chloride hexahydrate (1.35g, 5mmol) and fully dissolve it in 30mL ethylene glycol solution. Under stirring conditions, sodium acetate (NaAc, 3.6g) and polyethylene glycol-2000 (PEG-2000 1.0g) were added. Stirring was continued for 30 minutes, and the solution was transferred to a high temperature reactor at 200°C for 72 hours. After natural cooling, it was washed with deionized water and ethanol solution several times, and dried in vacuum at 60°C.
[0044] 2) Preparation of magnetic ferroferric oxide / rich aminated mesoporous silica composite material using polylysine as template (MMS / P NPs)
[0045] Dissolve 100 mg of sodium lauroyl sarcosinate into 10 mL of citric acid / sodium citrate buffer solution (0.1 M, pH 5.2) at room temperature, and add 150 μL of ε-polylysine (20 wt%) to the solution. At this time, the solution immediately turned into an emulsion, forming polylysine / s...
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[0054] Example 2
[0055] 1) Preparation of magnetic ferroferric oxide
[0056] Hydrothermal method was used to synthesize Fe3O4 nanoparticles (Fe 3 O 4 NPs). The specific method is as follows: Weigh ferric trichloride hexahydrate (1.35 g, 5 mmol) and fully dissolve it in 40 mL of ethylene glycol solution. Add sodium acetate (NaAc, 1.8g) and sodium citrate trihydrate (Na 3 Cit 0.2g). Stirring was continued for 1 hour, and the solution was transferred to a high temperature reactor at 200°C for 20 hours. After natural cooling, it was washed with deionized water and ethanol solution several times, and dried in vacuum at 60°C.
[0057] 2) Preparation of magnetic ferroferric oxide / rich aminated mesoporous silica composite material using hyperbranched polyamide (PAMAM) as template (MMS / H NPs)
[0058] Will Fe 3 O 4 NPs solution (3mg / mL, 50mL) was mixed with 9mL hydrazine hydrate and treated with ultrasound for 30 min. The mixture was transferred to a three-necked flask, and 70 mL of dei...
Example Embodiment
[0063] Example 3
[0064] 1) Preparation of magnetic ferroferric oxide
[0065] Hydrothermal method was used to synthesize Fe3O4 nanoparticles (Fe 3 O 4 NPs). The specific method is as follows: Weigh ferric trichloride hexahydrate (1.35 g, 5 mmol) and fully dissolve it in 40 mL of ethylene glycol solution. Add sodium acetate (NaAc, 1.8g) and sodium citrate trihydrate (Na 3 Cit 0.2g). Stirring was continued for 1 hour, and the solution was transferred to a high temperature reactor at 200°C for 20 hours. After natural cooling, it was washed with deionized water and ethanol solution several times, and dried in vacuum at 60°C.
[0066] 2) Preparation of magnetic ferroferric oxide / rich aminated mesoporous silica composite material using chitosan as template
[0067] Will Fe 3 O 4 NPs solution (3mg / mL, 50mL) was mixed with 9mL hydrazine hydrate and treated with ultrasound for 30 min. The mixture was transferred to a three-necked flask, and 70 mL of deionized water was added. Subsequent...
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