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A Strain of Pseudomonas stutzeri and Its Application

A technology of Pseudomonas stutzeri and Bacillus, applied in the direction of application, bacteria, and fungicides, can solve the problems of long cycle of disease-resistant varieties, single resistance, and pesticide residues, and achieve good application prospects, fast growth, and high yield. The effect of large amount of spores

Active Publication Date: 2020-10-09
宁国市百立德生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease-resistant variety has a long cycle and a single resistance
The use of chemicals for chemical control takes a long time and a large amount of use, resulting in pesticide residues and environmental pollution; at the same time, the long-term use of chemical pesticides makes pathogenic bacteria resistant to them, resulting in decreased control efficiency or even failure

Method used

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  • A Strain of Pseudomonas stutzeri and Its Application
  • A Strain of Pseudomonas stutzeri and Its Application
  • A Strain of Pseudomonas stutzeri and Its Application

Examples

Experimental program
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Effect test

Embodiment 1

[0022] 1. Isolation and purification of Pseudomonas stutzeri strain YT2003

[0023] The Pseudomonas stutzeri strain YT2003 of the present invention is obtained from the soil by the dilution plate method and the plate streaking method. The separation method is as follows:

[0024] (1) Separation of bacilli: soil samples are collected, and plots of different types of crops are selected in different regions of the country, and the 5-point sampling method is adopted to collect appropriate amounts of soil around the plot and the central depth within the range of 10-20cm, etc. Mix well. Indicate the location, time and person of collection. Weigh 1g soil sample in 100mL sterile water, place it in a shaker at 30℃, shake at 150rpm for 10min, then incubate it in a water bath at 60℃ for 30min, take 100μL of 10-2, 10-3, 10-4 dilution for coating On the LB medium plate, each gradient was coated in three parallels. After culturing at 30°C for 2 days, the microbial colonies of different forms o...

Embodiment 2

[0036] Fermentation process of Pseudomonas stutzeri strain YT2003

[0037] LB liquid medium: The formula is: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH adjusted to 7.0-7.2.

[0038] Mass fermentation medium formula (mass percentage): soy flour 3.0%, corn flour 3.0%, light calcium carbonate 0.2%, sodium chloride 0.1%, the rest is water, pH 7.3.

[0039] Mass fermentation process of Pseudomonas stutzeri strain YT2003:

[0040] ①Bacterial seed liquid culture: pick a small amount of bacteria from the slope of the test tube from the Pseudomonas stutzeri strain YT2003, transfer to LB liquid medium, shake culture at 30℃ for 3-5 days, this is the seed liquid;

[0041] ② Mass solid fermentation: Inoculate a large amount of fermentation medium with the seed liquid inoculation ratio of 1:90. Place the inoculated raw materials in a fermentation room (30°C and relative humidity above 85%) for fermentation and culture for 3-5 days. After drying, the original Pseu...

Embodiment 3

[0043] Field efficacy test

[0044] This example provides related experiments on the control effect of Pseudomonas stutzeri strain YT2003 on watermelon fusarium wilt.

[0045] 1) Test medicine

[0046] Pseudomonas stutzeri strain YT2003 original powder (prepared in Example 2); 70% thiophanate methyl wettable powder (commercially available).

[0047] 2) Test crops and control objects:

[0048] The test crop was watermelon; the variety was Qilin 8424;

[0049] Control object: Fusarium wilt.

[0050] 3) Test site conditions, test design and arrangement

[0051] The test site was set up in the watermelon field in Xinhu Village, Wanglao Township, Dongying City, Shandong Province. The soil was clay soil, and fusarium wilt was severe in previous years. The cultivation conditions of the experimental sites are uniform.

[0052] The ratio of Pseudomonas spp. YT2003 original powder in this test facility is 1:200, 1:400, 1:600; 70% thiophanate-methyl WP 1:600; clean water is used as a control for a to...

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Abstract

The invention discloses a strain of Pseudomonas stutzeri and its application, and relates to the field of biological control of plant diseases. The bacterial strain is Pseudomonas stutzeri (pseudomonas stutzeri) YT2003, and the preservation number is CGMCC No.14674. The invention also discloses a biological control preparation comprising the Pseudomonas stutzeri strain YT2003 and a preparation method thereof. The Pseudomonas stutzeri strain of the invention has the characteristics of fast growth speed, wide action spectrum, strong stress resistance, rapid and large-scale colonization in plant rhizosphere, etc., and thus has good application prospects. The biological control preparation prepared by the Pseudomonas stutzeri strain can be used as biological pesticide or biological fertilizer to prevent and control various soil-borne plant bacterial diseases, including watermelon wilt and the like.

Description

Technical field [0001] The invention relates to the field of biological control of plant diseases, in particular to a strain of Pseudomonas stutzeri and its application. Background technique [0002] Plant diseases seriously affect the growth of agricultural and forestry crops, and even cause crop deaths, causing significant economic losses. For the control of plant diseases, conventional methods include traditional chemical control, breeding of disease-resistant varieties, and biological control. Disease-resistant varieties have a long cycle and single resistance. The long-term use of chemical control agents and the large amount of use have caused pesticide residues and environmental pollution; at the same time, the long-term use of chemical pesticides makes pathogens resistant to them, resulting in a decline in control effectiveness or even failure. The use of beneficial microorganisms to control plant diseases has broad prospects. [0003] Pseudomonas is an important part of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/27A01P3/00C12R1/38
CPCA01N63/10C12N1/205C12R2001/38
Inventor 牛兆颖牛赡光张俊生张淑静
Owner 宁国市百立德生物科技有限公司
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