Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Germanium Dioxide Nanozyme and Its Pesticide Detection Application

A germanium dioxide and nano-enzyme technology, applied in the field of analytical chemistry, can solve the problems of long operation time, limited large-scale application, and the detection of organophosphorus pesticides has not been reported, and achieves the effect of broad application prospects.

Active Publication Date: 2021-06-04
QINGDAO AGRI UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Germanium dioxide has not been reported to have imitation enzyme activity
However, these methods tend to have low sensitivity and long operation time, require special professionals, and rely on large and expensive instruments
These factors severely limit the large-scale application of the above method in the field of pesticide residue detection.
In summary, GeO with imitation enzyme activity 2 Nanozymes are novel in their field, and GeO-based 2 Organophosphorus pesticide detection by nanozyme

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Germanium Dioxide Nanozyme and Its Pesticide Detection Application
  • Germanium Dioxide Nanozyme and Its Pesticide Detection Application
  • Germanium Dioxide Nanozyme and Its Pesticide Detection Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Imitation peroxidase material GeO 2 Preparation of nanozymes:

[0031] 1.06 g GeO 2 The solid powder was added to 10 mL of sodium hydroxide solution, 1.46 g of cetyltrimethylammonium bromide (CTAB) was dissolved in 12 mL of distilled water, and the two solutions were stirred at room temperature for 12 h. The CTAB solution was then poured into GeO 2 In alkaline solution, stir at room temperature for 8 h. Finally, the mixture was transferred to an autoclave, maintained at 100 °C for 56 h, and then cooled to room temperature. That is, germanium dioxide (GeO) with a triangular structure is obtained 2 ) nanozymes. figure 1 GeO observed by transmission electron microscopy 2 TEM imaging of nanozymes.

Embodiment 2

[0033] GeO 2 Enzyme-like activity verification of nanozymes:

[0034] Experimental system a: The catalytic reaction system contains H 2 o 2 (0.15 mmol / L), the GeO obtained in the above examples 2 Nanozyme (100 µg / mL), organic chromogen TMB (0.5 mmol / L) in acetate buffer (pH 4.0, 100 mmol / L). After reacting at room temperature (25 °C) for 30 minutes, use a microplate reader to detect the absorbance at 450−800 nm;

[0035] Two other control experiments were done: GeO was not added to the catalytic reaction system of one of the control experiments b. 2 Nanozyme, after reacting for 30 minutes under the same conditions as the above-mentioned experimental system, the absorbance value was detected; the catalytic reaction system of another control experiment c was GeO 2Nanozyme (100 µg / mL) was placed in acetate buffer (pH 4.0, 100 mmol / L), and the absorbance value was detected after standing for 30 minutes under the same conditions as the above experimental system;

[0036] Such...

Embodiment 3

[0038] GeO 2 pH optimization of nanozyme imitation enzyme activity:

[0039] The catalytic reaction system contains H 2 o 2 (0.3 mmol / L), GeO 2 Nanozyme (100 µg / mL), organic chromogenic agent TMB (0.5 mmol / L) and different pH buffers (pH 1.0−2.0, glycine-hydrochloric acid buffer; pH 3.0−6.0, acetic acid-sodium acetate buffer; pH 6.5−8.0, phosphate buffer; pH 9.0−10.0, Tris−HCl buffer; pH 11.0−12.0, sodium bicarbonate-sodium hydroxide buffer). After reacting at room temperature (25°C) for 30 minutes, the absorbance at 650 nm was detected with a microplate reader. Such as image 3 As shown, GeO 2 Nanozymes exhibit peroxidase activity at acidic pH (pH 3.5−5.0), and the optimum pH is around 4.0.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the fields of nanocatalysis and analytical chemistry, specifically including germanium dioxide (GeO 2 ) nanozyme and its pesticide detection application. GeO 2 The nanozyme has peroxidase activity at acidic pH, and can catalyze the color reaction between hydrogen peroxide and organic chromogen. The present invention utilizes prepared GeO 2 Nanozyme coupled with acetylcholinesterase realizes the quantitative analysis of organophosphorus pesticides. The invention can be applied to fields such as pesticide residue analysis, biomedicine, and environmental monitoring.

Description

technical field [0001] The invention relates to the field of analytical chemistry, specifically including germanium dioxide nanozyme and its pesticide detection application. Background technique [0002] Nanozyme is a new type of mimetic enzyme, which has become the focus of attention due to its strong catalytic activity and potential applications in biosensing, food technology, environmental protection, etc. Nanozymes have overcome many shortcomings of natural enzymes, such as high price, easy inactivation and harsh storage conditions, etc., and have had a huge impact on the fields of biosensing, immunoassay, cancer diagnosis and treatment. [0003] So far, a variety of inorganic nanomaterials (including Co 3 o 4 , CeO 2 , V 2 o 5 , CuO, MnO 2 , CuS, FeS, noble metals, carbon (fullerene, graphene), metal-organic frameworks and their composite nanostructures) all exhibit imitation enzyme activity (including imitation peroxidase, imitation oxidase, imitation catalase ,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): B01J23/14B01J35/02C12Q1/46B01J35/00
CPCC12Q1/46B01J23/14B01J35/40
Inventor 韩磊梁欣
Owner QINGDAO AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com