High-sensitivity iron ion probe and application thereof

A high-sensitivity, iron-ion technology, applied in the field of fluorescent probes, can solve problems such as being unsuitable for mass detection and real-time detection, and achieve the effects of high yield, short time, and easy availability of raw materials

Active Publication Date: 2019-02-15
HUANGSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above method requires expensive instruments and professional inspectors, and is not suitable for mass detection and real-time detection

Method used

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  • High-sensitivity iron ion probe and application thereof
  • High-sensitivity iron ion probe and application thereof
  • High-sensitivity iron ion probe and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation of embodiment 1 iron ion probe

[0038] (1) Synthesis of Rhodamine 6G:

[0039] a, 5.0000g 3-ethylamino-4-methylphenol, 15.0000g phthalic anhydride and 4.8000g zinc chloride are placed in a mortar and ground, and poured into an eggplant-shaped flask after grinding evenly (the eggplant-shaped flask The size is selected according to the needs of use), heat the flask, keep the temperature at 165 ° C, and slowly stir the reaction for 1 hour, so that the mixture gradually melts and the color becomes darker. At this time, careful stirring is required. As the reaction progresses, the consistency gradually It becomes smaller, and then gradually becomes larger, and the reactant presents a metallic brown-purple color; then decompress and vacuumize, and remove the cork after 5 to 10 minutes until no phthalic anhydride sublimates in the bottle;

[0040] b. Dissolve the mixture obtained in step a in 120mL of N,N-dimethylformamide (DMF), then slowly drop into 1200mL ...

Embodiment 2

[0054] Ion response and selectivity of the iron ion probe of embodiment 2

[0055] Add 2940 μL of acetonitrile aqueous solution (the volume ratio of water and acetonitrile is 1:4) and 30 μL of iron ion probe solution (weigh 88 mg of the target product prepared in Example 1 to 50 mL) into 15 7 mL centrifuge tubes with a pipette gun. In a volumetric flask, dissolve with acetonitrile and make up to volume with acetonitrile), then add 60 μL of prepared 3mmol / L metal ion solution to 14 centrifuge tubes, each metal ion solution corresponds to a centrifuge tube, one of which does not contain The acetonitrile aqueous solution of the probe solution was used as a blank sample, and was labeled with label paper; the 14 metal ions were Ag + 、Al 3+ 、Ba 2+ , Ca 2+ 、Co 2+ 、Cu 2+ , Fe 3+ , Hg + 、K + , Mn + 、Na + 、Ni + , Pb + , Zn 2+ .

[0056] When adding Fe 3+ solution, it was found that the color of the solution in the centrifuge tube changed from light yellow to colorless, an...

Embodiment 3

[0060] Interference of embodiment 3 iron ion probe

[0061] In this example, Fe 3+ Whether it interferes with the response of other metal ions to detect ion interference, use a pipette gun to add 60 μL of Fe 3+ solution, and shake well. Join Fe 3+ After the solution, the colors of 14 centrifuge tubes all turned red, indicating that other metal ion solutions had no effect on the iron ion probe of the present invention.

[0062] Then detect the ultraviolet absorption situation of the solution in these 14 centrifuge tubes with the ultraviolet spectrophotometer, the wavelength is set at 300~600nm, the result is as follows Figure 4 shown. The results show that when adding Fe 3+ After solution, the solutions in all 14 centrifuge tubes showed obvious ultraviolet absorption peaks at 530nm, and the absorbance changed. Among them, Fe 3+ The absorbance is significantly higher than that of other metal ions and Fe 3+ The absorbance of other metal ions on Fe 3+ There is no interfer...

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Abstract

The invention relates to the technical field of fluorescent probes, specifically to a high-sensitivity iron ion probe and application thereof. The specific scheme is that the invention discloses a structural formula of the high-sensitivity iron ion probe, and further discloses application in detecting Fe<3+>. When the concentration of the Fe<3+> is 3 mmol/L, the minimum detection limit for Fe<3+>is 0.32 [mu]M, and the Fe<3+> undergoes complexation reaction. The iron ion probe synthesized by the invention has a simple synthesis process, easy availability of raw materials, simple reaction conditions, simple operation and high yield, and is highly selective to Fe<3+> among various common metal ions, and is not interfered by other common ions. The high-sensitivity iron ion probe realizes reaction type identification on the combination of Fe<3+> and is irreversible; the target probe can be used for naked eye detection of the Fe<3+> without any equipment. Moreover, the high-sensitivity ironion probe is short in detection time for the Fe<3+> and higher in sensitivity.

Description

technical field [0001] The invention relates to the technical field of fluorescent probes, in particular to a high-sensitivity iron ion probe and its application. Background technique [0002] Iron is one of the essential trace elements for the human body, accounting for about 0.006% of the body's weight. It is also the most important transition metal ion in organisms, and is a cofactor for many enzymatic reactions, providing oxygen-carrying capacity for heme, and in cells It plays a vital role in many physiological processes such as metabolism. At the same time, Fe element is the most important component of various enzymes such as myoglobin and hemoglobin. The source of iron in the human body is intake from food on the one hand, and release from red blood cells on the other hand. If the body is deficient in iron, it will affect the synthesis of myoglobin, etc., and show corresponding case characteristics, such as decreased immunity and infection resistance, weakened abili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/107G01N21/33G01N21/64G01N21/78G01N21/82
CPCC07D491/107G01N21/33G01N21/6428G01N21/78G01N21/82G01N2021/825
Inventor 李长江叶天卉潘乐周权黄飞
Owner HUANGSHAN UNIV
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