Ophiocordyceps sinensis mycelium powder and production method thereof

A technology for Cordyceps sinensis fungus and mycelium powder, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low degree of organicization, low yield of selenium-enriched cordyceps mycelium, and no selenium enrichment. Cordyceps sinensis mycelium powder industrialization and other problems to overcome the inhibition effect

Active Publication Date: 2019-02-22
马兰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the prior art, the yield of submerged fermentation of selenium-enriched Cordyceps sinensis mycelium is low, and there are also defects such as low utilization rate of inorganic selenium, low selenium content of the product, and low degree of organicization
Therefore, there is no report on the industrialization of selenium-enriched Cordyceps sinensis mycelium powder by liquid submerged fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of slant seeds: DCH slant medium: 5.0% sucrose, 0.3% yeast powder, 1.0% tryptone, 1.0% agar powder, with an inorganic ion solution of pH 5.6-6.0 (0.5 grams of magnesium sulfate, 1 gram of potassium dihydrogen phosphate , 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate, and 1000 ml of distilled water). After the culture medium is prepared, subpackaged, and sterilized, it is inclined to make an inclined plane. Inoculate the slant medium with the activated CICC No. 14088 Cordyceps sinensis strain, and culture it at 15-16°C for 40 days.

[0033] Preparation of shake flask seeds: shake flask medium: glucose 1.5%, corn flour 2.0%, molasses 0.5%, potato 6.0%; silkworm chrysalis powder 2.5%, soybean sprout powder 1.0%, yeast powder 0.5%, bran 1.0%; sulfuric acid Magnesium 0.02%, potassium dihydrogen phosphate 0.02%, and the rest is water. First cut potatoes ...

Embodiment 2

[0038] Preparation of slant seeds: DCH slant medium: 5.0% sucrose, 0.3% yeast powder, 1.0% tryptone, 1.0% agar powder, with an inorganic ion solution of pH 5.6-6.0 (0.5 grams of magnesium sulfate, 1 gram of potassium dihydrogen phosphate , 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate, and 1000 ml of distilled water). After the culture medium is prepared, subpackaged and sterilized, the inclined plane is made, and the activated Cordyceps sinensis strain of CICC number 14088 is inoculated on the inclined plane medium, and cultured at 17-18° C. for 35 days.

[0039] Preparation of shake flask seeds: shake flask medium: glucose 1.5%, corn flour 2.0%, molasses 1.0%, potato 6.0%; silkworm chrysalis powder 2.5%, soybean sprout powder 1.0%, yeast powder 0.5%, bran 1.0%; sulfuric acid Magnesium 0.02%, potassium dihydrogen phosphate 0.02%, and the rest is water. First cut pota...

Embodiment 3

[0044] Preparation of slant seeds: DCH slant medium: 5.0% sucrose, 0.3% yeast powder, 1.0% tryptone, 1.0% agar powder, with an inorganic ion solution of pH 5.6-6.0 (0.5 grams of magnesium sulfate, 1 gram of potassium dihydrogen phosphate , 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate, and 1000 ml of distilled water). After the culture medium is prepared, subpackaged and sterilized, the inclined plane is made, and the activated Cordyceps sinensis strain of CICC number 14088 is inoculated on the inclined plane medium, and cultured at 17-18° C. for 35 days.

[0045] Preparation of shake flask seeds: shake flask medium: glucose 1.8%, corn flour 2.0%, molasses 0.5%, potato 6.0%; live silkworm chrysalis 6.0%, soybean sprout powder 1.0%, yeast powder 0.5%, bran 1.0%; sulfuric acid Magnesium 0.02%, potassium dihydrogen phosphate 0.04%, and the rest is water. First, cut potat...

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Abstract

The invention relates to the technical field of biological fermentation, and discloses ophiocordyceps sinensis mycelium powder and a production method thereof. The method concretely comprises the following steps of taking ophiocordyceps sinensis bacteria as strains, carrying out submerged fermentation to produce selenium-rich ophiocordyceps sinensis mycelium by adding inorganic selenium or/and organic selenium liquid into a culture medium, wherein the yield of dry mycelia is 28-35 g/L, the total selenium contained in the mycelia is 25-1,350 mu g/g, the w/% of organic selenium to total seleniumis greater than 92%, and the two types of mycelia further contain effective ingredients such as 4-8% of cordycepic acid, 5-8% of cordyceps polysaccharide and 0.03-0.17% of adenosine, and smashing themycelium into 80-100 mesh to prepare a finished product of the mycelium powder. Experiments prove that the product has the functions of oxidation resistance, cancer resistance and prevention, fatigueresistance, immunity reinforcement, lung and kidney tonifying, liver protection for detoxification and the like, and the ophiocordyceps sinensis mycelium powder has practicability no matter used as amedicine, a health care food or a nutritional supplement.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a selenium-enriched Cordyceps sinensis mycelium powder produced by submerged liquid fermentation and a production method thereof. Background technique [0002] Cordyceps sinensis (Cordyceps sinesis) lives in alpine meadows at an altitude of 3000-5200 meters on the Qinghai-Tibet Plateau, and is a complex of insects and fungi. The relationship between the insect and the fungus is that after the Cordyceps sinensis (Ophiocordyceps sinesis) infects the larvae of bat moths, it gradually transforms the nutrients in the larvae into mycelium and fills the entire insect tissue, leaving only a layer of insect skin. Keeping the shape of insects in dry soil for several months is called winter worms. When the temperature and humidity are suitable in summer, rod-shaped fruiting bodies (sperms) grow from the head of the worms and are exposed to the ground, which is called summer ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/38C12R1/645
CPCC12N1/14C12N1/38
Inventor 马兰陈大伟刘小朋
Owner 马兰
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