Liquid culture medium suitable for tender stems of Dendrobium officinale and preparation method thereof
A technology of liquid culture medium and dendrobium candidum, which is applied in the direction of culture medium, botany equipment and methods, horticultural methods, etc., can solve the problems of unsatisfactory arginine content and polysaccharide content, and achieve the improvement of arginine content and polysaccharide content The effect of content
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
preparation example Construction
[0095] The present invention also discloses a method for preparing a liquid culture medium suitable for the tender stems of Dendrobium officinale, comprising the following steps:
[0096] S1, add apple extract, potato extract, major elements including potassium, nitrogen, phosphorus, magnesium, trace elements including iodine, boron, molybdenum, iron salts and organic matter at room temperature for 5-7 times Amount of water is mixed to obtain product A;
[0097] S2, predissolve 2, 4-D and NAA in absolute ethanol to prepare a 0.5-2 mg / ml solution to obtain product B;
[0098] S3, mix products A and B in tissue culture bottles, sterilize under high-temperature steam for 20-30 minutes, and cool to obtain.
[0099] Concrete formula embodiment is as follows:
Embodiment 1
[0101] Formula: sucrose 20g / L, potassium nitrate 50mg / L, ammonium nitrate 80.04mg / L, magnesium sulfate 246.47mg / L, calcium chloride 147.02mg / L, potassium iodide 166.01mg / L, boric acid 61.83mg / L, manganese sulfate 223.01 mg / L, Zinc Sulfate 287.54mg / L, Sodium Molybdate 241.95mg / L, Copper Sulfate 249.68mg / L, Cobalt Chloride 237.93mg / L, Disodium EDTA 372.25mg / L, Ferrous Sulfate 278.03 mg / L, inositol 100mg / L, glycine 2mg / L, thiamine hydrochloride 0.1mg / L, pyridoxine hydrochloride 0.5mg / L, niacin 0.5mg / L, potassium dihydrogen phosphate 75mg / L, potato extract Extract 10%, apple extract 5%, 2,4-D 2mg / L, NAA 0.5mg / L.
Embodiment 2
[0103] Formula: sucrose 20g / L, potassium nitrate 50mg / L, ammonium nitrate 80.04mg / L, magnesium sulfate 246.47mg / L, calcium chloride 147.02mg / L, potassium iodide 166.01mg / L, boric acid 61.83mg / L, manganese sulfate 223.01 mg / L, Zinc Sulfate 287.54mg / L, Sodium Molybdate 241.95mg / L, Copper Sulfate 249.68mg / L, Cobalt Chloride 237.93mg / L, Disodium EDTA 372.25mg / L, Ferrous Sulfate 278.03 mg / L, inositol 100mg / L, glycine 2mg / L, thiamine hydrochloride 0.1mg / L, pyridoxine hydrochloride 0.5mg / L, niacin 0.5mg / L, potassium dihydrogen phosphate 75mg / L, potato extract Extract 20%, apple extract 5%, 2,4-D 2mg / L, NAA 0.5mg / L.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More