A kind of qthipp37 gene and its application

Abstract

The present invention discloses a gene and its application of Divinia chinensis QtHIPP37, the nucleotide sequence of which is shown in SEQ ID NO.1, and the amino acid sequence encoded by the gene is shown in SEQ ID NO.2. Based on the establishment of a high-efficiency regeneration system for the bulbils on the leaves of Dieffenbachia variegata and the analysis of the transcriptome, the present invention clones the QtHIPP37 gene and constructs an overexpression vector, and transfers it into the model plant Nicotiana tabacum, and the transgenic tobacco is resistant to drought stress. It proves that the QtHIPP37 gene can improve the stress resistance performance of plants such as drought resistance and salt-alkali resistance at the molecular level, and has a good application prospect in the application of plant biotechnology.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to a gene of evergreen QtHIPP37 and its application. Background technique [0002] Drought and salinity are currently global problems that seriously restrict agricultural production. China itself is a country with severe water shortage and very scarce fresh water resources. Due to the impact of climate warming and excessive use of chemical fertilizers, drought, water shortage and soil compaction Salinization and other problems are becoming more and more serious, posing a great threat to agricultural, forestry and horticultural production. In particular, many flowering and horticultural plants are cultivated in protected areas and greenhouses all year round. Watering and irrigation consumes a lot of resources, manpower and material resources, and water resources urgently need efficient At the same time, problems such as salinization of cultivated soil ar...

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Problems solved by technology

[0005] Ornithogalum thyrsoides is a perennial bulbous flower of the genus Ornithogalum thyrsoides in the family Liliaceae. It has the advantages of strong vitality, wide adaptability, strong reproductive ability, and easy cultivation, especially for drought, saline-alkali and other adversity stresses. Strong stress resistance, but its molecular mechanism needs to be further studied

[0006] Molecular chaperones are key proteins that regulate the safe transport of metal ions in cells, and are closely related to plant resistance; heavy metal-associated isoprenylated plant protein (HIPP) is a plant-specific Molecular chaperones containing two conserved sites of metal binding site (HMA) and C-terminal prenylation site (Caax), studies in Arabidopsis and wheat have shown that in heavy metals, drought, cold, salt Under stress conditions such as alkali and disease, the expression of this gene is significantly increased, especially the gene can interact with the stress-related zinc finger protein transcription factor ATHB29 through its heavy metal-associated binding site (HMA). It plays an important role in the dehydration stress caused by drought threat, and can improve the resistance of plants to dehydration stress and drought at the molecular level; however, the HIPP gene has only been reported in a few plants such as Arabidopsis and wheat, while It has not been reported in Dieffenbachia, Liliaceae, bulbous flowers and other plants.

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