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Preparation method of high-stability carbon dot-silicon dioxide composite particles

A technology of silicon dioxide and composite particles, applied in chemical instruments and methods, material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of deviation of test results and affect the accuracy of test results, and achieve uniform size, stable luminescence properties, Easy-to-retouch effect

Inactive Publication Date: 2019-04-26
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most fluorescence detection methods are based on the change of the fluorescence intensity of the probe with a single emission wavelength. This kind of single-color fluorescent probe detection method will be affected by the detection environment in the actual application process, which will cause large deviations in the detection results.
For example, temperature, concentration of fluorescent probes, offset of light source or detector, and impurities in the sample to be tested will all affect the accuracy of test results

Method used

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  • Preparation method of high-stability carbon dot-silicon dioxide composite particles
  • Preparation method of high-stability carbon dot-silicon dioxide composite particles
  • Preparation method of high-stability carbon dot-silicon dioxide composite particles

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1: Synthesis process of carbon dots-silica composite particles with different emission wavelengths

[0032]Add 50ml of ethanol to five 250ml round bottom flasks respectively, add 1ml of water respectively, add 1ml of tetramethylammonium hydroxide aqueous solution with mass fraction of 1%, 2%, 3%, 4%, 5%, respectively, and finally add 1ml TEOS. The silica nanoparticles doped with tetramethylammonium hydroxide were obtained after stirring and reacting in a water bath at 30° C. for 3 hours. Then the reaction solution was centrifuged at 10,000 rpm for 15 min, redispersed with ethanol and washed by centrifugation for two times, then dispersed with ultrapure water and washed by centrifugation for two times. The obtained solid was dried in an oven at 60 °C for 6 hours, and finally ground into powder thoroughly with a mortar. The obtained solid powder was calcined at 300°C for 3 hours to obtain carbon dot-silicon dioxide composite particles with emission wavelengths a...

Embodiment 2

[0033] Example 2: Synthesis process of carbon dots-silica composite particles with different fluorescence quantum yields

[0034] 50ml of ethanol was added to five 250ml round bottom flasks respectively, 1ml of water was added respectively, 1ml of tetramethylammonium hydroxide aqueous solution with a mass fraction of 1% was added respectively, and finally 1ml of TEOS was added respectively. The silica nanoparticles doped with tetramethylammonium hydroxide were obtained after stirring and reacting in a water bath at 30° C. for 3 hours. Then the reaction solution was centrifuged at 12000 rpm for 10 min, redispersed with ethanol and washed by centrifugation for two times, then dispersed with ultrapure water and washed by centrifugation for two times. The obtained solid was dried in an oven at 70 °C for 4 hours, and finally ground into powder thoroughly with a mortar. The obtained solid powder was calcined at 200°C, 300°C, 400°C, 500°C, and 600°C for 3 hours to obtain carbon dots...

Embodiment 3

[0035] Example 3.: Stability experiment of carbon dots-silicon dioxide composite particles

[0036] The carbon dot-silicon dioxide composite particles and fluorescein (commonly used fluorescent dye) synthesized in Example 1 with a wavelength of 455nm were irradiated under a fluorescence microscope respectively, and it was found that the fluorescence of fluorescein was almost completely quenched within 60s, while The carbon dot-silicon dioxide composite particles are quenched within 5%. It shows that the carbon dots-silicon dioxide composite particles prepared by the present invention have good photobleaching resistance compared with fluorescent dyes that are easily photobleached, and the corresponding fluorescence intensity change curve is shown in Figure 4 .

[0037] The carbon dot-silicon dioxide composite particles synthesized in Example 1 with an emission wavelength of 455nm were respectively dissolved in phosphate buffer solutions with pH values ​​of 3, 4, 5, 6, 7, 8, 9...

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Abstract

The invention discloses a preparation method of high-stability carbon dot-silicon dioxide composite particles and belongs to the technical field of carbon materials and fluorescent composite nanomaterials. The method comprises the steps as follows: firstly, quaternary ammonium base doped spherical silicon dioxide nanoparticles are synthesized with quaternary ammonium base as a catalyst and a doping carbon source and TEOS as a silicon source; performing high-temperature calcinations in a muffle furnace to enable the doping quaternary ammonium base is dehydrated and condensed in situ in a silicon dioxide substrate to form fluorescent carbon dots to obtain the carbon dot-silicon dioxide composite particles. The preparation process is simple, and the prepared composite particles have the advantages of uniform size, high photobleaching resistance and chemical stability, good biocompatibility and easy surface modification and the like and can be used as a universal fluorescent reference material to be composited with other fluorescent materials to construct a dual-emission ratio type fluorescent probe.

Description

technical field [0001] The invention belongs to the technical field of carbon materials and fluorescent composite nanomaterials, and specifically relates to a highly stable carbon dot with silicon dioxide as the matrix and quaternary ammonium alkali as the doped carbon source, which can be easily and efficiently synthesized by high-temperature calcination with adjustable emission wavelength- The method of silica fluorescent composite particles, and its application to the construction of dual-emission colorimetric fluorescent probes. Background technique [0002] As a new type of fluorescent material, carbon dots have attracted extensive attention due to their excellent optical properties, low toxicity, water solubility, chemical inertness and biocompatibility, and have been widely used in optical devices, biological imaging, photocatalysis and fluorescence transmission. senses etc. Carbon dots have become a promising alternative fluorescent material to traditional organic d...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/65G01N21/64
CPCC09K11/025C09K11/65G01N21/6428G01N21/643
Inventor 王敏黄臻臻滕士勇杨文胜韩延东郭子龙
Owner JILIN UNIV
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