Method for separating and extracting amino acid mother liquor

An extraction method and amino acid technology, which are applied in the field of amino acid mother liquor treatment, can solve problems such as unfavorable treatment of large-scale mother liquor, high cost of culture medium raw materials, limited amount of waste water used, etc., and achieve good film hanging effect, simple and feasible method, and pollutant concentration. reduced effect

Inactive Publication Date: 2019-04-26
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Studies have shown that amino acid industrial wastewater can be used to produce animal protein for feed. For example, the Chinese patented technology "Method for Fermenting Debariae hansenii Using High Salt Amino Acid Wastewater" discloses a method of recycling amino acid wastewater for fermentation A method for producing bacterial protein, the method uses waste water to prepare a medium, and the components of the medium are high-salt amino acid sewage: 125-330g / L, carbon source: 30-100g / L, potassium dihydrogen phosphate (KH 2 PO 4 ): 5-15g / L, yeast powder: 0-10g / L, magnesium sulfate (MgSO 4 ): 3-9g / L, calcium carbonate (CaCO 3 ): 0.75-2g / L, ferrous sulfate (FeSO 4 ): 1.5-4g / L, and the pH of the medium is 3.5-7.5. This method effectively treats wastewater and prepares bacterial protein; however, this method also has some defects: the cost of medium raw materials is high, and the amount of wastewater used is limited, etc. defect
[0006] The applicant's previous patented technology "CN107267427A, a method for recycling threonine mother liquor" used Bacillus subtilis, Bacillus licheniformis and Scenedesmus obliquus to treat the mother liquor, and the effect was good, but Bacillus subtilis, lichen Bacillus and Scenedesmus obliques are domesticated, and additional medium components need to be added, the process is complicated, the cost is increased, and it is not conducive to the treatment of large-scale mother liquor

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  • Method for separating and extracting amino acid mother liquor
  • Method for separating and extracting amino acid mother liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The separation and extraction method of threonine mother liquor, it comprises the steps:

[0029] Extraction and separation of threonine by high performance liquid chromatography: to contain Na + The positive resin is a stationary phase adsorbent, to which the mother liquor of threonine is added, the chromatographic separation temperature is 65°C-85°C, and the flow rate is controlled at 80-85m 3 / h, for chromatographic separation, using water as the eluent, the flow rate is controlled at 90-95m 3 / h, to separate the residual sugar, pigment and inorganic salt in the mother liquor of threonine for the production of fertilizer; the liquid after chromatographic separation is evaporated and concentrated, and then centrifuged to obtain threonine crystals and waste liquid;

[0030] Candida utilis was inoculated into YPD liquid medium for cultivation to obtain Candida utilis seed liquid, and the cell concentration was 1×10 9 CFU / ml;

[0031] Lactobacillus casei was inoculate...

Embodiment 2

[0036] The separation and extraction method of amino acid mother liquor, it comprises the steps:

[0037] Extraction and separation of threonine by high performance liquid chromatography: to contain Na + The positive resin is a stationary phase adsorbent, to which the mother liquor of threonine is added, the chromatographic separation temperature is 65°C-85°C, and the flow rate is controlled at 80-85m 3 / h, for chromatographic separation, using water as the eluent, the flow rate is controlled at 90-95m 3 / h, to separate the residual sugar, pigment and inorganic salt in the mother liquor of threonine for the production of fertilizer; the liquid after chromatographic separation is evaporated and concentrated, and then centrifuged to obtain threonine crystals and waste liquid;

[0038] Candida utilis was inoculated into YPD liquid medium for cultivation to obtain Candida utilis seed liquid, and the cell concentration was 2×10 9 CFU / ml;

[0039] Lactobacillus casei was inoculate...

Embodiment 3

[0044] The threonine mother liquor used in the present invention is the raffinate after extracting threonine from the fermentation broth, and detects the indicators of pollutants in the threonine mother liquor before treatment, after chromatographic treatment and after biochemical treatment. For specific processes, refer to Example 1, See Table 1 for details:

[0045] Table 1

[0046]

[0047] As shown in Table 1 above, after chromatographic treatment, the content of threonine in the waste liquid decreased significantly, a certain amount of threonine was extracted, the total yield increased, and the economic benefit was improved; the residual sugar, pigment and Inorganic salts can also be used in fertilizers; after biochemical treatment, the pollutants and crude protein content in the waste liquid are significantly reduced, and the treated liquid can be used as water source for field irrigation, reducing the environmental protection pressure of enterprises.

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Abstract

The invention belongs to the technical field of amino acid mother liquor treatment and discloses a method for separating and extracting amino acid mother liquor. The method comprises the following steps: 1) extracting and separating threonine through chromatography; 2) preparing a fermented liquid; 3) preparing an anabaena flos-aquae fluid; 4) treating effluent. According to the invention, high performance liquid chromatography is firstly adopted for separating residual threonine in mother liquor; the separated component can be used for fertilizers; a certain amino acid product can be acquired; pollutant concentration in effluent can be reduced; biochemical treatment time can be shortened; waste of resources can be eliminated.

Description

technical field [0001] The invention belongs to the technical field of amino acid mother liquor treatment, and in particular relates to a method for separating and extracting amino acid mother liquor. Background technique [0002] my country is a big country in the production and consumption of amino acids. In the production of chemical, pharmaceutical, food and other industries, a large amount of waste mother liquor containing amino acids is often discharged, which contains a variety of amino acids, residual sugar, fermentation intermediate products, ammonium salts, etc., which are characterized by high COD and high NH 3 The characteristics of -N, and it is acidic. After being discharged into water bodies, it will seriously pollute the environment, and it is difficult to treat. In order to solve the problem of wastewater pollution and make effective use of wastewater resources, in the traditional production process, the bacterial protein in the waste amino acid mother liquo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34C07C227/40C07C229/22C12R1/245C12R1/72C12R1/01C02F103/36
CPCC12N1/16C12N1/20C02F3/341C02F3/347C07C227/40C02F2103/36C07C229/22
Inventor 赵兰坤李德衡徐庆阳张建华刘元涛杨雪曹博超
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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