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Flexible liposome cosmetic containing active polypeptides and preparation method thereof

A flexible liposome, active polypeptide technology, applied in cosmetic preparations, cosmetics, cosmetic preparations, etc., can solve the problem of difficult passage of active ingredients

Active Publication Date: 2019-05-03
深圳高尚科美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Compared with proteins, the molecular weight of these active peptides has been greatly reduced, and some have been modified hydrophobically. However, due to the barrier function of the stratum corneum of the skin, most of the active ingredients are difficult to pass through.

Method used

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  • Flexible liposome cosmetic containing active polypeptides and preparation method thereof
  • Flexible liposome cosmetic containing active polypeptides and preparation method thereof
  • Flexible liposome cosmetic containing active polypeptides and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1 The preparation of the flexible liposome containing active polypeptide of the present invention

[0096] Accurately weigh soybean lecithin, sodium deoxycholate or polysorbate 80, and vitamin E according to the formula, dissolve them in chloroform / ethanol or ethanol respectively, dissolve and mix them in a 250ml pear-shaped bottle, and spin evaporate at room temperature for 2 hours, and the obtained The films were placed in a vacuum oven overnight. The next day, distilled water and appropriate amount of hydrophobized modified polypeptide were added, and a 400w probe was sonicated for 30 minutes to obtain a blank transfersome solution. Slowly add an appropriate amount of acetyl octapeptide-8 or palmitoyl tetrapeptide or palmitoyl pentapeptide or nonapeptide-1 or acetyl tetrapeptide-5 or myristoyl pentapeptide-17, etc. After incubation at room temperature for 40 minutes, the obtained The mother liquor is filtered through a 0.2 μm polycarbonate membrane for ma...

Embodiment 2

[0240] Embodiment 2 The transdermal effect verification of the flexible liposome containing active polypeptide of the present invention

[0241] The in vitro transdermal experiment adopts an improved single-chamber Franz diffusion cell, and mouse skin is used as the in vitro transdermal experimental skin to compare the transdermal absorption results of different flexible liposomes containing active polypeptides, and samples are taken at different time points. The HPLC method was used to measure the concentration of the drug in the transdermal receiving solution, and the cumulative transdermal amount was calculated.

[0242] Test example 2-1 Verification of transdermal effect of flexible liposomes containing acetyl hexapeptide-8

[0243] 1. Materials

[0244] Sample: the flexible liposome freeze-dried powder containing acetyl hexapeptide-8 (1-1,1-2,1-3) prepared by test example 1 with polysorbate 80 as surfactant, PAL-DP7 modification , 1-4) and flexible liposomes containing ...

Embodiment 3

[0321] Embodiment 3 The stability research of the flexible liposome freeze-dried powder containing active polypeptide of the present invention

[0322] The flexible liposome lyophilized powder containing active polypeptide prepared in Example 1 was subjected to stability research (including three aspects such as accelerated test and long-term test). The samples were placed in a drug stability test chamber at 37°C for accelerated testing. They were placed for 1, 2, 3, and 6 months respectively, and the properties of the freeze-dried powder were measured, including color, content, and solubility. The results are shown in Table 13. Then put the samples in a drug stability test box at 25°C for long-term tests, and place them for 3, 6, 9, 12, 15, 18, 21, and 24 months respectively to detect the color, HPLC content, and solubility of the freeze-dried powder. The results are shown in Table 14.

[0323] Table 13 Stability Accelerated Test (37° C.) of flexible liposome freeze-dried po...

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Abstract

The invention belongs to the field of cosmetics, and specifically relates to a flexible liposome cosmetic containing active polypeptides and a preparation method thereof. Due to barrier effects of cuticle of skin epidermis, the skin is difficult to be penetrated by most of active ingredients of cosmetics containing active polypeptides. In order to solve the problem, the invention provides a flexible liposome cosmetic containing active polypeptides. The flexible liposome cosmetic containing the active polypeptides is prepared by containing active polypeptides in flexible liposomes modified by hydrophobic modifying polypeptides, so that, transmittance of the cosmetic is effectively improved; and thus, the developed cosmetic is easier to absorb as well as better in anti-oxidizing and anti-aging effects. Therefore, the cosmetic has broad application prospects.

Description

technical field [0001] The invention belongs to the field of cosmetics, and in particular relates to a flexible liposome cosmetic containing active polypeptides and a preparation method thereof. Background technique [0002] Polypeptides are a class of compounds that are condensed by two or more amino acids and connected by peptide bonds. It is an intermediate substance of proteins. A peptide containing less than 10 amino acids is called an oligopeptide, and a peptide with more than 50 amino acids is called a protein. Polypeptides have high biological activity, which can regulate various physiological activities and biochemical reactions of body cells, and are also called active biological polypeptides. [0003] In the defense and care of natural skin aging, active biological peptides also play a unique and important physiological role, such as proliferation of skin tissue cells, cell chemotaxis and migration, repair and regeneration, angiogenesis and reconstruction, pigmen...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/67A61K8/14A61K8/35A61Q7/00A61Q19/00A61Q19/08
Inventor 杨莉魏于全
Owner 深圳高尚科美生物科技有限公司
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