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Sporosarcina strain and application thereof in deodorization of livestock and poultry manure

A technology for sporococcus spores and livestock and poultry manure, which is applied in the direction of bacteria, fungi, and animal husbandry wastewater treatment, can solve the problems of high cost, complicated reaction, secondary pollution, etc., and achieves stable and reliable products, simple and efficient preparation process. Effect

Inactive Publication Date: 2019-06-11
张俊哲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical deodorization effect is good, but there are disadvantages such as high cost, complicated reaction, secondary pollution, and difficulty in resource utilization of feces, so it is difficult to popularize and apply

Method used

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  • Sporosarcina strain and application thereof in deodorization of livestock and poultry manure
  • Sporosarcina strain and application thereof in deodorization of livestock and poultry manure
  • Sporosarcina strain and application thereof in deodorization of livestock and poultry manure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Isolation and identification of Bacillus sporogenes LY1 (CGMCC No. 16759)

[0028] Sampling: 20 to 30 black soldier fly larvae were washed and disinfected with alcohol and normal saline, then dissected and taken out the intestinal tract, ground in a sterilized mortar, and added an appropriate amount of sterile water to prepare a bacterial suspension.

[0029] Enrichment: Take 0.5ml of bacterial suspension from the mortar with a 1ml pipette, and put them into the shake flasks of 100ml of liquid enrichment medium marked in (1), put the shaker flasks into the shaker at 220 rpm / min, 30°C, shake for 48 hours (repeated 5 times); (2) stand for 48 hours in a marked Erlenmeyer flask with 200ml liquid enrichment medium. With a 1ml pipette, take 1ml of culture medium for dilution, and take 10 -3 , 10 -4 , 10 -5 , 10 -6 Four gradients, each with two parallels, were spread on solid enriched medium plates for shake flask culture, and poured with solid medium for stati...

Embodiment 2

[0039] Example 2: Application effect of Bacillus sporogenes LY1 (CGMCC No. 16759) in deodorization of chicken house air

[0040] Liquid culture medium: peptone 10g, beef extract 15g, NaCl 15g, distilled water 1L, pH 7.2, sterilized at 121°C for 20min.

[0041] After activation, Bacillus sporogenes LY1 was inserted into the liquid medium with 5% inoculum, and cultured at 37°C and 180rpm for 18-24h, and the bacterial content reached 10 8 cfu / mL, collect the fermented bacteria liquid, divide it into 2 parts on average, take 1 part of it and add yeast, lactic acid bacteria, bacillus, photosynthetic bacteria, etc. to make a mixed inoculum. Lactic acid bacteria 10%, Bacillus 10%, photosynthetic bacteria 10%, the above two bacterial liquids were sprayed evenly in two chicken houses of the same breeding scale and under the same management conditions, at different directions and about 1.5m above the ground, 5 sampling points were selected, and the ammonia gas detection instrument was ...

Embodiment 3

[0044] Example 3: Application of Saccharomyces sporogenes LY1 (CGMCC No. 16759) in the deodorization of livestock and poultry manure and kitchen waste

[0045] Culture medium: peptone 10g, beef extract 15g, NaCl 15g, distilled water 1L, pH 7.2, sterilized at 121°C for 20min.

[0046] After activation of Bacillus sporogenes LY1, cultured at 37°C and 180rpm for 18h, the bacterial content reached 10 8 cfu / mL, collect the fermented bacterial liquid, inoculate 5mL into 1L lunch boxes containing 500g chicken manure, pig manure, cow manure and kitchen waste, mix well, seal with plastic wrap, add 5mL sterile Water was used instead of bacterial liquid, and three replicates were set for each group, and the ammonia concentration was measured after being placed at room temperature for 5 days. The results are shown in Table 3 below.

[0047] Table 3 Deodorization effect of Bacillus sporogenes LY1 on livestock and poultry manure

[0048] Measurement index (ammonia concentration: ...

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Abstract

The invention relates to a Sporosarcina strain and application thereof in deodorization of livestock and poultry manure, and belongs to the field of microorganisms. The Sporosarcina patent strain hasa preservation number of CGMCC NO.16759 and is preserved in the China General Microbiological Culture Collection Center on November 21, 2018. The strain can be applied to biological deodorization of the livestock and poultry manure, can effectively reduce the release amount of malodorous gases such as ammonia gas and hydrogen sulfide in the livestock and poultry manure, reduces the generation of the malodorous gases from the source, has a stable deodorization effect, is less influenced by the temperature, and still has a better deodorization effect under the condition of normal temperature.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a sporococcus spore and its application in the deodorization of livestock and poultry manure. Background technique [0002] With the large-scale and intensive development of the livestock and poultry breeding industry, the problem of environmental pollution has become more and more serious, and the management difficulty has increased. The stench of livestock and poultry manure will be generated during the stacking process, which will lead to environmental deterioration. The odor components produced by livestock and poultry manure are complex and can usually be divided into the following four categories: First, volatile sulfur-containing compounds, such as H 2 S, CH 4 S, thioethers, etc., mainly from the reduction of sulfate by microorganisms and the metabolism of sulfur-containing amino acids; the second is nitrogen-containing compounds, such as NH 3...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F11/02B01D53/84B01D53/58C12R1/01C12R1/645C12R1/125C02F103/20
CPCY02A50/20
Inventor 张俊哲刘执平张兴晓陈国忠
Owner 张俊哲
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