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Micro-fluidic device and method for manipulating and capturing micro-particles and cells based on non-contact dielectrophoresis force

A microfluidic device and a technology of dielectrophoresis, which are applied in the methods of stress-stimulated microbial growth, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problems of complex process, high chip cost, chip pollution, etc. , to achieve the effect of reducing manufacturing costs, reducing bonding accuracy requirements, and accurate sample flow rate

Active Publication Date: 2019-06-21
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a microfluidic device and method based on non-contact dielectrophoresis force control to capture microparticles and cells, so as to solve the problems of traditional metal contact dielectrophoresis chip contamination of samples, complicated process and relatively low chip cost. high problem

Method used

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  • Micro-fluidic device and method for manipulating and capturing micro-particles and cells based on non-contact dielectrophoresis force
  • Micro-fluidic device and method for manipulating and capturing micro-particles and cells based on non-contact dielectrophoresis force
  • Micro-fluidic device and method for manipulating and capturing micro-particles and cells based on non-contact dielectrophoresis force

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Preparation of microfluidic chip

[0053] 1.1 First, for the structural part of the microfluidic chip, use AutoCAD drawing software to draw the required structural graphics, make a mask plate, and use a four-inch single crystal silicon wafer as the substrate to perform glue coating photolithography, reactive ion etching, and glue removal Cleaning, gluing photolithography, deep reactive ion etching, etch out the height of the chip channel, and obtain a silicon wafer mold with a microstructure.

[0054] 1.2 Place the silicon wafer mold and the open centrifuge tube containing 10 μL of fluorosilane in a vacuum drying oven, vacuum to negative pressure to vaporize the fluorosilane, and let the mold stand in the fluorosilane vapor for 5-6 hours. In a ventilated place, the drying box was opened, and after ventilating for 1 hour, the silicon wafer was taken out. The purpose of this step is to deposit a layer of organic matter on the surface of the silicon wafer to fac...

Embodiment 2

[0056] Example 2 Using the microfluidic chip prepared in Example 1 to perform deflection sorting of polystyrene microspheres of different sizes

[0057] Put the microfluidic chip 300 packaged in Example 1 into a vacuum pot to evacuate for 30 minutes, then take out the microfluidic chip 300, suck 100 μL of DEP conductive solution with a pipette gun, and then insert the tip of the pipette into the microfluidic chip 300 The electrode solution inlet 6 of the electrode solution was left to stand for 10 minutes, and the DEP conductive liquid was sucked into the first and second liquid electrode channels by relying on the negative pressure in the closed electrode channel. The effect of liquid electrode channel part not filled with conductive liquid and filled with conductive liquid is as follows Figure 4 Shown in A and B.

[0058]The polystyrene microspheres with a diameter of 5 μm and 10 μm were washed with deionized water, shaken and centrifuged twice, and then resuspended in the...

Embodiment 3

[0062] Using the microfluidic chip 300 provided in Example 1 and the method provided in Example 2, the polystyrene microsphere sample was replaced with circulating tumor cells H446, and the circulating tumor cells were washed with PBS (containing 0.05% Tween) and centrifuged twice. , and then resuspended in the DEP buffer (8.5% sucrose [wt / vol], 0.3% glucose [wt / vol]) prepared by us, the conductivity was adjusted to 10ms / m, and the electric field parameter output by the function generator was 6V, 100KHz, the waveform is unified as a square wave. The effect of capturing circulating tumor cell H446 after starting the electrode is as follows: Figure 6 As shown, the simulation effect of the potential electric field distribution in the dielectrophoretic area is as follows Figure 7 shown.

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Abstract

The invention provides a micro-fluidic device for manipulating and capturing micro-particles and cells based on non-contact dielectrophoresis force. The device comprises a sampling pump, a micro-syringe, a micro-fluidic chip and a waste collector which are sequentially connected, a glass base layer and a polydimethylsiloxane chip layer are fitted to form the micro-fluidic chip, the polydimethylsiloxane chip layer comprises a sampling opening, a sampling channel, a sheath flow sampling opening, a sheath flow sampling channel, a first liquid electrode channel, a second liquid electrode channel,a target product collection channel, a waste collection channel and a sample discharging port, the first liquid electrode channel and the second liquid electrode channel are closed annular, arranged on two sides of a sample separation channel, and a high-frequency and high-voltage non-uniform field is applied onto the sample separation channel. The invention further provides a method for manipulating and capturing the micro-particles and the cells based on the non-contact dielectrophoresis force. The device and the method have the advantages of high separation efficiency and flux, simplicityin operation and low coat.

Description

technical field [0001] The invention relates to the field of biological detection, and more specifically relates to a microfluidic device and method for manipulating and capturing microparticles and cells based on non-contact dielectrophoretic force. Background technique [0002] Cancer is still one of the diseases with the highest mortality rate in the world. In 2008, 7.6 million people died from cancer (13% of all deaths), and by 2030 it is likely to exceed 13 million. Early diagnosis and treatment of primary cancer and effective treatment of metastatic cancer are the key to fighting cancer. [0003] Circulating tumor cells (Circulating Tumor Cells, CTCs) are a type of tumor cells that come from the primary tumor and enter the blood circulation system. Such cells can transfer through the blood circulation system and reach other organs to achieve tumor metastasis, so CTCs are closely related to the occurrence, development and metastasis of tumors. In view of the importan...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/42
Inventor 贾春平金鎏王艳敏郜晚蕾周洪波赵辉齐同赵建龙
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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