Construction method of mutant strain for increasing metabolism yield of filamentous fungi through form optimization and orientation

Abstract

The invention relates to a construction method of a mutant strain for increasing the metabolism yield of filamentous fungi through form optimization and orientation. The method comprises the steps that the filamentous fungi are cultured in a liquid culture medium; whole genomes are extracted; an amplification-aim form gene segment of a primer in the whole genomes of the filamentous fungi is designed; the aim gene segment is connected with a carrier for constructing a carrier for regulating and controlling form gene expression; the filamentous fungi are converted through the carrier, and resistance genes on the carrier are used for screening converters; the mutant strain is inoculated into a seed bottle, after culture is conducted for a time period, filtering is conducted for collecting hyphae, and after dyeing, the form is observed through a microscope; after seeds are transferred into a fermentation culture medium, sampling is conducted at certain time intervals, filtering is conducted for collecting hyphae, and after dyeing, the form is observed through the microscope; the titer of the product is measured, and the high-yield strain is screened. The hyphae of transformed fungi have multiple branches, the branches wind and gather into balls, the branches of extraglomerular hyphae are short, the balls of the fungi are compact and even in size, and the viscosity of a fermentationsolution is low.

Description

technical field [0001] The invention relates to a method for constructing mutant strains for improving the metabolic yield of filamentous fungi through morphological optimization and orientation, and belongs to the field of biotechnology. Background technique [0002] Humans have a long history of using fungi, but it was not until after World War I, with the rise of liquid fermentation technology, that filamentous fungal fermentation gradually flourished in the industrial field. Now filamentous fungi have been widely used in the fermentation industry for antibiotics (penicillin, cephalosporin, etc.), organic acids (citric acid, gluconic acid, fumaric acid, etc.) and enzyme preparations (amylase, pectinase, cellulase, etc.) The production of these products has great demand in the fields of medicine, food and other industries. [0003] During the fermentation process, the morphological changes of filamentous fungi are complex, showing distinct morphological characteristics at...

AI Technical Summary

Problems solved by technology

However, the morphology optimization of filamentous bacteria cannot be achieved fundamentally only by process control, and the molecular biology modification of related genes is the fundamental measure to achieve the control of filamentous bacteria fermentation morphology

Images