The invention relates to a construction method of a
mutant strain for increasing the
metabolism yield of filamentous fungi through form optimization and orientation. The method comprises the steps that the filamentous fungi are cultured in a
liquid culture medium; whole genomes are extracted; an amplification-aim form
gene segment of a primer in the whole genomes of the filamentous fungi is designed; the aim
gene segment is connected with a carrier for constructing a carrier for regulating and controlling form
gene expression; the filamentous fungi are converted through the carrier, and resistance genes on the carrier are used for screening
converters; the
mutant strain is inoculated into a seed
bottle, after culture is conducted for a time period, filtering is conducted for collecting hyphae, and after
dyeing, the form is observed through a
microscope; after seeds are transferred into a
fermentation culture medium, sampling is conducted at certain time intervals, filtering is conducted for collecting hyphae, and after
dyeing, the form is observed through the
microscope; the
titer of the product is measured, and the high-yield strain is screened. The hyphae of transformed fungi have multiple branches, the branches wind and gather into balls, the branches of extraglomerular hyphae are short, the balls of the fungi are compact and even in size, and the
viscosity of a fermentationsolution is low.