A kind of microbial inoculum for effective treatment of uranium pollution and its application
A technology for microbial inoculum and uranium contamination, applied in the field of radioactive contamination bioremediation, can solve the problems of easy shedding of fixation, increased difficulty, complicated operation, etc., and achieves remarkable treatment effect, improved mineralization ability, and small aerobic environment.
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Embodiment 1
[0028] Affected by uranium mining, the water body near a uranium mine in Xinjiang is seriously polluted by uranium, and the microbial preparation of the present invention is used for repair through the following steps:
[0029] (1) Detection: Take an appropriate amount of uranium-contaminated water body samples, use ICP-MS to detect the uranium concentration in the water body is 8.5mg / L, calculate the required amount of bacteria according to the volume of the repaired water body, and ensure that the amount of bacteria is 1g / L.
[0030] (2) pH adjustment: the pH of the water body is detected to be 4, and the pH is adjusted to 5-6 with NaOH solution.
[0031] (3) Mixing: Mix Bacillus thuringiensis, Bacillus cereus and Saccharomyces cerevisiae in a weight ratio of 4:3:3 evenly. Three kinds of bacterium can be purchased directly in China General Microorganism Culture Collection Management Center (CGMCC), adopt Bacillus thuringiensis (Bacillus thuringiensis 016), Bacillus cereus (B...
Embodiment 2
[0037] Firstly, Bacillus thuringiensis, Bacillus cereus and Saccharomyces cerevisiae were activated by culturing in a shaker at 30°C for 12 hours at a shaking speed of 160 rpm, and then transferred to 100 mL of LB medium for 24 hours respectively. Afterwards, the bacteria were collected by centrifugation at 6000 rpm, and washed three times with sterilized 1 mM NaCl solution to wash away the residual medium components, and three kinds of bacteria were collected.
[0038] (1) Mix the collected three kinds of bacteria according to the weight ratio of 5:4:2, and resuspend them in a certain volume of U(VI) solution (the concentration of uranium is 100mg / L, 200mg / L, 300mg / L), so that the bacterial cell concentration is 1g / L (dry weight). Then place it in a constant temperature shaker at 30°C and 160rpm to react, take samples every 24 hours to measure the remaining uranium concentration in the solution, collect the precipitate by centrifugation, wash it with sterilized distilled wate...
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