A kind of microbial inoculum for effective treatment of uranium pollution and its application

A technology for microbial inoculum and uranium contamination, applied in the field of radioactive contamination bioremediation, can solve the problems of easy shedding of fixation, increased difficulty, complicated operation, etc., and achieves remarkable treatment effect, improved mineralization ability, and small aerobic environment.

Active Publication Date: 2021-07-20
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In related fields, most of the technologies for heavy metal remediation do not include the remediation of uranium pollution. For example, the patent document "A Biochemical Composite Soil Heavy Metal Pollution Fixing Adsorbent and Its Application" (application number: 201610488744.1, publication date: 2016.11 .23) A biochemical method is disclosed to remediate heavy metal pollution in soil. Its working principle is to add photosynthetic bacteria, actinomycetes, yeast, iron powder, zeolite, mercapto chitosan and other substances to the soil, and enhance the Adsorption of cadmium, chromium, copper, mercury, arsenic, and lead, but the patent document does not indicate whether this method has adsorption effect on uranium. If the method is directly used for the adsorption of uranium, since the adsorption is usually accompanied by With desorption reaction, this method is also prone to shedding phenomenon for the fixation of uranium, resulting in the re-migration of uranium.
In addition, there are also a small number of patents that mention the restoration of heavy metals by Bacillus thuringiensis, such as the patent document "A microbial product and its manufacturing method for simultaneously controlling heavy metal pollution and insect damage" (application number: 201610713954.6, publication date: 2017.01.04) , its working principle is that Brevibacillus lateralsporosa and Bacillus thuringiensis act as insecticides, Bacillus subtilis decomposes the necrotic roots of crops to increase soil fertility, and other microorganisms are used to enrich heavy metals in the soil. This patent document is aimed at Dry soil polluted by heavy metals in agriculture, but the ability of Bacillus thuringiensis to mineralize hexavalent uranium has not been involved, let alone its effect on the restoration and treatment of uranium-contaminated water bodies
The patent document "A Method for Treating Heavy Metals in Soil" (application number: 201610250782.3, publication date: 2016.07.27) discloses a microbial agent to treat 8 kinds of pollution in soil, including cadmium, mercury, arsenic, copper, lead, chromium, nickel and zinc. method, but it does not involve the treatment of uranium pollution. In addition, the acquisition of its beneficial bacteria must first be implanted in plants, and then obtained from cow dung for use by cattle. The acquisition period is long and the operation is complicated. Increased the difficulty of practical application

Method used

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Experimental program
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Effect test

Embodiment 1

[0028] Affected by uranium mining, the water body near a uranium mine in Xinjiang is seriously polluted by uranium, and the microbial preparation of the present invention is used for repair through the following steps:

[0029] (1) Detection: Take an appropriate amount of uranium-contaminated water body samples, use ICP-MS to detect the uranium concentration in the water body is 8.5mg / L, calculate the required amount of bacteria according to the volume of the repaired water body, and ensure that the amount of bacteria is 1g / L.

[0030] (2) pH adjustment: the pH of the water body is detected to be 4, and the pH is adjusted to 5-6 with NaOH solution.

[0031] (3) Mixing: Mix Bacillus thuringiensis, Bacillus cereus and Saccharomyces cerevisiae in a weight ratio of 4:3:3 evenly. Three kinds of bacterium can be purchased directly in China General Microorganism Culture Collection Management Center (CGMCC), adopt Bacillus thuringiensis (Bacillus thuringiensis 016), Bacillus cereus (B...

Embodiment 2

[0037] Firstly, Bacillus thuringiensis, Bacillus cereus and Saccharomyces cerevisiae were activated by culturing in a shaker at 30°C for 12 hours at a shaking speed of 160 rpm, and then transferred to 100 mL of LB medium for 24 hours respectively. Afterwards, the bacteria were collected by centrifugation at 6000 rpm, and washed three times with sterilized 1 mM NaCl solution to wash away the residual medium components, and three kinds of bacteria were collected.

[0038] (1) Mix the collected three kinds of bacteria according to the weight ratio of 5:4:2, and resuspend them in a certain volume of U(VI) solution (the concentration of uranium is 100mg / L, 200mg / L, 300mg / L), so that the bacterial cell concentration is 1g / L (dry weight). Then place it in a constant temperature shaker at 30°C and 160rpm to react, take samples every 24 hours to measure the remaining uranium concentration in the solution, collect the precipitate by centrifugation, wash it with sterilized distilled wate...

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PUM

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Abstract

The invention provides a microbial bacterial agent for effectively treating uranium pollution and its application. The composition of the microbial agent includes Bacillus thuringiensis, Bacillus cereus, and Saccharomyces cerevisiae, and the organic synergy between the strains improves the mineralization ability of uranium, shortens the time for good crystal minerals, and at the same time, the uranium concentration is high It can still play a role in high or other extreme environments; its raw materials are cheap and easy to obtain, it has the advantages of high repair efficiency and less environmental disturbance, and can effectively repair uranium-contaminated water bodies. The application of the present invention realizes the direct mineralization of hexavalent uranium into hexavalent uranium minerals with larger particle size and less exposure to aerobic environment through non-reductive mineralization method, which can exist stably, with better mineralization effect and improved restoration effect , and expanded the scope of application, simple operation, easy to scale, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of bioremediation of radioactive pollution, in particular to a microbial agent for effectively treating uranium pollution and its application. Background technique [0002] At present, the nuclear energy industry is in a period of rapid development. However, a large amount of uranium-containing waste and uranium-containing wastewater will be produced in the process from uranium mining and smelting to enrichment. According to statistics, there are more than 2×10 9 Tons of uranium tailings are a long-term threat to human health. In addition, because phosphate fertilizers and potassium fertilizers also contain certain radioactive elements, the use of chemical fertilizers in agriculture will also bring certain uranium pollution. These discarded uranium will be enriched into the human body through the food chain. The radioactivity, high toxicity and carcinogenicity of hexavalent uranium will cause systemic diseases mainl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20G21F9/18C12R1/07C12R1/085C12R1/865
CPCC12N1/20G21F9/18
Inventor 林璋卫亚丽刘学明邓洪刘炜珍
Owner SOUTH CHINA UNIV OF TECH
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