Detonating agent used for aerobic fermentation of biogas residue and preparation method thereof
A technology of aerobic fermentation and detonator, applied in the field of agricultural waste recycling, can solve the problems of slow heating of materials, poor quality of aerobic fermentation products, short high temperature period, etc., and achieves time extension, efficiency improvement and harmless level. Effect
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Embodiment 1
[0047] (1) Mix Bacillus, Lactobacillus, Enterococcus, Brevibacillus, Aromatobacter, and Acetobacter according to the ratio of 20:44:15:8:8:5, and add it to the microbial culture medium (into 1L of water Add 6g of beef extract, 15g of peptone, 6g of sodium chloride, and prepare by sterilizing at 121°C for 15 minutes), and incubate at 25°C for 24 hours to obtain a composite microbial agent; the number of viable bacteria per gram of the compound microbial agent can reach 1× 10 9 above;
[0048] (2) Mix biochar, wood acetic acid and microbial culture medium evenly in the ratio of 10g: 15ml: 75ml to obtain a high-efficiency immobilization medium.
[0049] (3) Take 1g of compound microbial agent and place it in a 250ml Erlenmeyer flask, add 8ml of high-efficiency immobilization medium to ensure that each gram contains 1×10 beneficial bacteria 9 Above, adsorb and fix in a 150rpm shaker at 25°C for 24h, centrifuge at 4000r / min for 15min, pour off the supernatant, and freeze-dry the ...
Embodiment 2
[0051] (1) Mix Bacillus, Lactobacillus, Enterococcus, Brevibacillus, Aromatobacter, and Acetobacter according to the ratio of 20:44:13:9:9:5, and add it to the microbial culture medium (into 1L of water Add 6 g of beef extract, 15 g of peptone, 6 g of sodium chloride, and sterilize at 121° C. for 15 minutes to prepare), and incubate at 25° C. for 28 hours to obtain a composite microbial bacterial agent.
[0052] (2) Mix biochar, wood acetic acid and microbial culture medium evenly in the ratio of 8g: 18ml: 72ml to obtain high-efficiency immobilized culture medium.
[0053] (3) Take 1g of compound microbial agent and place it in a 250ml Erlenmeyer flask, add 10ml of high-efficiency immobilization medium to ensure that each gram contains more than 1 billion beneficial bacteria, absorb and fix it in a 150rpm shaker at 25°C for 30h, 4000r / Centrifuge for 20 minutes at 1 min, pour off the supernatant, and freeze-dry the lower layer of sediment to become the primer for biogas residu...
Embodiment 3
[0055] (1) Mix Bacillus, Lactobacillus, Enterococcus, Brevibacillus, and Aroma-like bacteria at a ratio of 20:45:15:10:10 (excluding Acetobacter), and add them to the microbial culture medium (to each Add 6 g of beef extract, 15 g of peptone, and 6 g of sodium chloride into 1 L of water, prepare by sterilizing at 121° C. for 15 minutes, and incubate at 25° C. for 26 hours to obtain a composite microbial agent.
[0056] (2) Mix biochar, wood acetic acid, and microbial culture medium evenly in the ratio of 10g: 20ml: 70ml to obtain a high-efficiency immobilized medium.
[0057] (3) Take 1g of compound microbial agent and place it in a 250ml Erlenmeyer flask, add 10ml of high-efficiency immobilization medium to ensure that each gram contains more than 1 billion beneficial bacteria, absorb and fix it in a 150rpm shaker at 25°C for 30h, 4000r / Centrifuge at 1 min for 15 min, pour off the supernatant, and freeze-dry the lower layer to become the priming agent of the present inventio...
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