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A kind of silica nanometer gene delivery system and its preparation method and application

A technology of silica and nanomaterials, applied in the biological field, can solve the problems of few reports of successful DNA transfection, and achieve the effect of simple and easy preparation method, smaller tumor burden and obvious therapeutic effect

Active Publication Date: 2021-10-29
北斗生命科学(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Research on mesoporous calcium silicate nanoparticles in the field of gene delivery has also been carried out. By modifying cationic polymers such as polylysine and PEI on the surface of mesoporous silicon, mesoporous silicon can successfully transfect a variety of cells in vitro, but Successful DNA transfection in vivo has rarely been reported

Method used

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  • A kind of silica nanometer gene delivery system and its preparation method and application
  • A kind of silica nanometer gene delivery system and its preparation method and application
  • A kind of silica nanometer gene delivery system and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1: Synthesis of rod-shaped silica with an aspect ratio of 2.5

[0034] In a typical synthesis, tetraethyl orthosilicate (TEOS) was introduced using the block copolymer Pluronic P123 diluted in an acidic aqueous medium as a template. By controlling the concentration of the reactants and optimizing the reaction conditions, the rod-shaped silica is obtained. The specific method is as follows: 0.8g Pluronic P123 was dissolved in 30ml 2M HCl, and 1.72g TEOS was added dropwise. At this time, the concentrations of P123 and TEOS in the solution were 2.67% and 5.73%, respectively. After uniform stirring for 10 minutes, transfer the solution to a microwave digestion apparatus, react at 40°C for 2 hours, and react at 100°C for 1 hour. The obtained precipitated product was collected by centrifugation, using an ethanol solution containing 1% HCl, and then reacted for 1 hour at an environment of 80 degrees using a microwave digestion instrument to remove the template in the ...

Embodiment 2

[0035] Example 2: Synthesis of rod-shaped silica with an aspect ratio of 6.4

[0036] 0.168g of Pluronic P123 was dissolved in 30ml of 2M HCl, and 0.35g of TEOS was added dropwise. At this time, the concentrations of P123 and TEOS in the solution were 0.56% and 1.17%, respectively. After uniform stirring for 10 minutes, transfer the solution to a microwave digestion apparatus, react at 40°C for 2 hours, and react at 100°C for 1 hour. The obtained precipitated product was collected by centrifugation, using an ethanol solution containing 1% HCl, and then reacted for 1 hour at an environment of 80 degrees using a microwave digestion instrument to remove the template in the product, washed the product several times with ethanol, and finally stored in 4 degrees ethanol solution. The appearance characteristics of the product were characterized and analyzed by transmission electron microscopy, and mesoporous nanorods with a length of 1.78 μm and a width of 278 nm were obtained.

Embodiment 3

[0037] Example 3: Synthesis of silica nanowires with an aspect ratio of 21.4

[0038]0.08g of Pluronic P123 was dissolved in 30ml of 2M HCl, and 0.17g of TEOS was added dropwise. At this time, the concentrations of P123 and TEOS in the solution were 0.27% and 0.57%, respectively. After uniform stirring for 10 minutes, transfer the solution to a microwave digestion apparatus, react at 40°C for 2 hours, and react at 100°C for 1 hour. The obtained precipitated product was collected by centrifugation, using an ethanol solution containing 1% HCl, and then reacted for 1 hour at an environment of 80 degrees using a microwave digestion instrument to remove the template in the product, washed the product several times with ethanol, and finally stored in 4 degrees ethanol solution. The appearance characteristics of the product were characterized and analyzed by transmission electron microscopy, and mesoporous nanowires with a length of 1.80 μm and a width of 84 nm were obtained.

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Abstract

The invention discloses a silicon dioxide nanomaterial prepared by hydrolyzing TEOS under acidic conditions with P123 / F127 (tri-block copolymer) as a surfactant and TEOS (tetraethyl orthosilicate) as a silicon source. to make. The aspect ratio of the silicon dioxide nanometer material is 2.5-21.4, and has a one-dimensional structure. The aspect ratio of the silica nanorod material in the silica nano-gene delivery system of the present invention can be adjusted by reactant concentration, and its structure can be regulated as required. The one-dimensional silica material of the present invention has good biocompatibility, high transfection efficiency and low toxicity when used as a gene carrier; the one-dimensional silica material and nucleic acid (such as plasmid DNA) are formulated into a mixture, passed through the abdominal cavity Injected into mice, the plasmid DNA can be efficiently and long-term expressed; the preparation method of the one-dimensional silicon dioxide material and the pharmaceutical composition of the present invention is simple and easy, and has a high degree of controllability.

Description

technical field [0001] The invention relates to the field of biotechnology, and relates to a gene delivery system, in particular to a nano silicon dioxide-based gene delivery system and its preparation method and application. Background technique [0002] Gene therapy is to introduce normal genes or therapeutic genes into target cells through genetic engineering, and achieve the purpose of treating diseases through gene expression. Gene therapy has brought new treatment options for many congenital and acquired diseases such as tumors. [0003] Viral vectors are a common gene carrier, which use the ability of viruses to infect host cells to introduce foreign genes into host cells. Generally include lentivirus (lentivirus), adenovirus vector (AV), adeno-associated virus vector (AAV), retrovirus, etc. Although viral vectors have high DNA transfection efficiency, the capacity of viral vectors to load genes is limited, and the virus has potential biological safety hazards due t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K39/395A61K47/04B82Y30/00B82Y40/00C01B33/18
CPCA61K39/395A61K47/02A61K48/0008A61P35/00B82Y30/00B82Y40/00C01B33/18C01P2004/16C01P2004/64
Inventor 赵静蔡镓鹂陈国创王志勇钱志荣
Owner 北斗生命科学(广州)有限公司
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