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A kind of immobilized biological reaction filter plate and its preparation method and application

A technology of biological reaction and filter plate, which is applied in the direction of using microorganisms, chemical instruments and methods, fixed on or in inorganic carriers, etc., can solve the problems of inability to maximize the efficiency of microbial treatment, poor mass transfer performance of embedding balls, The treatment effect is strongly dependent on other issues, to achieve the effects of increased bioavailability, strong adhesion, and reduced treatment time

Active Publication Date: 2021-07-16
WUHU YIZE SMALL & MEDIUM ENTERPRISE PUBLIC SERVICE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with natural polymer gel carrier, the mechanical strength of immobilized particles made of organic polymer gel carrier is larger than that of natural polymer gel carrier, but its mass transfer performance is not as good as that of natural polymer gel Immobilized particles made of carriers, and after embedding, they are more active against microorganisms, which can easily cause the inactivation of immobilized microorganisms
[0006] In addition, most of the traditional embedded and immobilized microorganisms are porous pellet preparations with small specific surface area and small contact area with the substrate, and most of the microorganisms are embedded and immobilized inside the polymer carrier, and the treatment effect is strongly dependent on or Limited by the mass transfer performance of the carrier, the mass transfer performance of the embedding sphere formed by solidification and crosslinking of the polymer carrier is poor, and the microbial treatment efficiency cannot be maximized
At the same time, the continuous treatment of substrate solution per unit volume requires a large amount of immobilized microbial preparations as fillers, resulting in a large hydraulic retention time, slow treatment effect and low efficiency.

Method used

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  • A kind of immobilized biological reaction filter plate and its preparation method and application
  • A kind of immobilized biological reaction filter plate and its preparation method and application
  • A kind of immobilized biological reaction filter plate and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Prepare and apply the immobilized biological reaction filter plate according to the following method:

[0039] 1. Raw material preparation and prefabrication

[0040] The degree of polymerization of pure PVA is 2000, and the degree of alcoholysis is 87%.

[0041] Sludge enrichment and domestication of sewage treatment bacteria:

[0042] (A) Sterile initial culture medium is used to domesticate and enrich sewage treatment bacteria, and the activated sludge from the sewage treatment plant is inserted into the fermentation tank with a mixed liquid suspended solids concentration (MLSS) of 2.19g / L. Stir culture at 50rpm in the solution to ensure constant culture conditions: temperature 14°C, pH=7.0, dissolved oxygen 2.0mg / L; the concentration of ammonium nitrogen in the initial culture solution is 250mg / L, and the chemical oxygen demand is 100mg / L;

[0043] (B) Cultivate until the MLSS reaches 8g / L, replace the acclimatization culture medium every 6 hours wit...

Embodiment 2

[0055] Embodiment 2: Prepare and apply the immobilized biological reaction filter plate according to the following method:

[0056] 1. Raw material preparation and prefabrication

[0057] Suspension culture of chlorella alone to prepare algae suspension culture for sewage treatment, the algae cell density in the algae culture suspension is 1.22×10 5 stop culture when cells / mL;

[0058] The degree of polymerization of pure PVA is 6000, and the degree of alcoholysis is 89%.

[0059] 2. The preparation of the immobilized biological reaction filter plate includes the following specific steps:

[0060] (1) Heat the colorless transparent glass raw material to 950°C and keep it warm. After the liquid glass is completely melted, use a multi-layer spray deposition plate preparation device to prepare a deposited glass plate. The spray deposition process parameters are: 10MPa high-pressure argon as the atomizing gas, The vertical spraying distance is 35cm, the inner diameter of the ca...

Embodiment 3

[0070] Embodiment 3: Prepare and apply the immobilized biological reaction filter plate according to the following method:

[0071] 1. Raw material preparation and prefabrication

[0072] Separate suspension culture of Nannochloropsis, Botrytis, and Scenedesmus to prepare the suspension culture medium of sewage treatment algae, centrifuge separately after entering the plateau stage, and resuspend in pure water after mixing according to the wet weight ratio of 2:2:3, and the algae cells in the resuspension liquid Density is 6.35×10 5 per mL, the degree of polymerization of pure PVA is 4000, and the degree of alcoholysis is 88%.

[0073] Sludge enrichment and domestication of sewage treatment bacteria:

[0074] (A) Adopt aseptic initial culture liquid to carry out domestication and enrichment to sewage treatment bacteria, in the fermenter, the activated sludge is inserted into the culture liquid with 100rpm stirring culture according to the concentration of mixed liquid suspen...

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Abstract

The invention discloses an immobilized biological reaction filter plate and its preparation method and application. The immobilized biological reaction filter plate includes a deposition glass plate and microorganisms, and adopts a porous deposition glass plate formed after glass droplet cooling and deposition as a supporting carrier. The specific surface area under the same volume is large, and the contact area with the substrate liquid after loading the PVA film is also large. The microorganisms are fixed in the PVA film layer, the bioavailability is greatly improved, and the treatment efficiency is improved. The pore size of the deposition glass plate is moderate, taking into account the fluidity and hydraulic retention time of permeation filtration, and reducing the processing time per unit volume of substrate while ensuring the elimination rate of ammonia nitrogen. The modified PVA membrane has a stable effect of microorganism immobilization, and has little influence on the activity of microorganisms. When the immobilized biological reaction filter plate is applied to sewage treatment, the number of filter plate layers can be flexibly adjusted according to the ammonia nitrogen level of the sewage, the treatment intensity can be changed, unnecessary waste can be reduced, high-ammonia nitrogen sewage can be treated efficiently, and treatment costs can be reduced.

Description

technical field [0001] The invention relates to the field of immobilized microorganisms, in particular to an immobilized biological reaction filter plate and its preparation method and application. Background technique [0002] Microbial immobilization technology refers to the biological immobilization technology that uses physical or chemical methods to immobilize dominant microorganisms on a specific carrier at high density and maintain activity for a certain period of time, and can also meet specified requirements under suitable growth conditions. The basic starting point of microbial immobilization technology is to improve the efficiency and stability of the treatment process based on the dominant microbial population, increase the microbial density per unit volume, and eliminate the problem of microbial loss. Compared with the traditional activated sludge method, which has low microbial biomass, prone to sludge swelling and loss, and poor adaptability to wastewater qual...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N1/36C02F3/34C02F3/32C02F3/02C02F9/14C02F101/16
CPCC02F1/001C02F3/02C02F3/322C02F3/325C02F3/34C02F2101/16C12N1/36C12N11/14Y02W10/10
Inventor 孙有俊孙冬梅范彩云鲍芳丽
Owner WUHU YIZE SMALL & MEDIUM ENTERPRISE PUBLIC SERVICE CO LTD
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