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Preparation method of photoelectrochemical immunosensor for detecting cardiac troponin I

A cardiac troponin and immune sensor technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problem of long detection time of chemiluminescence immunoassay, complex and cumbersome operation of enzyme-linked immunoassay, poor controllability of fluorescence analysis, etc. problems, to achieve the effect of promoting the transfer of electrons, reducing other interference effects, and good biocompatibility

Active Publication Date: 2019-11-19
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many cTnI detection methods, such as chemiluminescence method (cardiac troponin luminol chemiluminescence immunoassay detection method, 031317979), electrochemical immunosensor method (a nitrogen-sulfur double-doped graphene oxide label The preparation method and application of the sandwich-type immunosensor, 201910144057.1), fluorescence colorimetry (a fluorescent immunochromatographic kit for the quantitative detection of troponin I / creatine kinase isoenzyme / myoglobin, 201110451642.X) , ELISA method (double-antibody sandwich ELISA detection kit for human cardiac troponin I, 201710797247.4), etc., but the operation of ELISA is complicated and cumbersome; fluorescence analysis has poor controllability and high toxicity; chemiluminescence immunoassay detection time is long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 Preparation of photoelectrochemical sensor

[0049] (1) Preparation of tungsten trioxide material

[0050] Dissolve 1.0 g of tungsten chloride in 35 mL of absolute ethanol, stir at room temperature for 1 h, transfer the mixed solution to an autoclave and react at 120 °C for 4 h, cool to room temperature naturally after the reaction, and then The product was washed 3 times with absolute ethanol and ultrapure water respectively. After washing, the product was dried at 40 °C for 10 h to obtain a sea urchin-shaped tungsten trioxide material;

[0051] (2) Preparation of bismuth sulfide nanorod materials

[0052] Dissolve 1.5 g of bismuth nitrate pentahydrate in 20 mL of ethylene glycol, stir at room temperature for 20 min, and use this solution as solution A; dissolve 1.2 g of sodium sulfide nonahydrate in 20 mL of ultrapure water, and stir at room temperature for 10 min min, this solution is used as solution B; dissolve 1.8g urea in 10 mL ultrapure water, sti...

Embodiment 2

[0073] Embodiment 2 Framework of photoelectrochemical sensor

[0074] (1) Preparation of tungsten trioxide material

[0075] Dissolve 1.5 g of tungsten chloride in 40 mL of absolute ethanol, stir at room temperature for 1.5 h, transfer the mixed solution to an autoclave at 160 °C for 5 h, and cool to room temperature naturally after the reaction. The product was washed 3 times with absolute ethanol and ultrapure water respectively. After washing, the product was dried at 50 °C for 11 h to obtain a sea urchin-shaped tungsten trioxide material;

[0076] (2) Preparation of bismuth sulfide nanorod materials

[0077] Dissolve 1.8 g of bismuth nitrate pentahydrate in 25 mL of ethylene glycol, stir at room temperature for 25 min, and use this solution as solution A; dissolve 1.3 g of sodium sulfide nonahydrate in 30 mL of ultrapure water, and stir at room temperature for 15 min min, this solution is used as solution B; dissolve 1.8g urea in 30 mL ultrapure water, stir well at room ...

Embodiment 3

[0098] Example 3 Construction of Photoelectrochemical Immunosensor

[0099] (1) Preparation of tungsten trioxide material

[0100] Dissolve 2.0 g of tungsten chloride in 50 mL of absolute ethanol, stir at room temperature for 2 h, transfer the mixed solution to an autoclave and react at 180 °C for 8 h, cool to room temperature naturally after the reaction, and then The product was washed 3 times with absolute ethanol and ultrapure water respectively. After the washing, the product was dried at 60 °C for 12 h to obtain a sea urchin-shaped tungsten trioxide material;

[0101] (2) Preparation of bismuth sulfide nanorod materials

[0102] Dissolve 2.0 g of bismuth nitrate pentahydrate in 30 mL of ethylene glycol, stir at room temperature for 30 min, and use this solution as solution A; dissolve 1.5 g of sodium sulfide nonahydrate in 50 mL of ultrapure water, and stir at room temperature for 20 min min, this solution is used as solution B; take 2.0g urea and dissolve it in 40 mL ...

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Abstract

The invention relates to a preparation method of a photoelectrochemical immunosensor for detecting cardiac troponin I. Immunoassay and photoelectric test are separated by constructing a split-type photoelectrochemical sensor, so that the photoelectric test and the immune recognition process of biomolecules do not interfere with each other. Bismuth sulfide nanorod modified tungsten trioxide is usedas a substrate material to provide basic photoelectric response, the band gap structures of bismuth sulfide nanorods and tungsten trioxide are matched, and visible light can be well utilized. Then, characteristic immune recognition of an antigen and an antibody is carried out in a 96 micro-well plate, ascorbic acid-coated mesoporous silica packaged by cadmium sulfide is used to label a cardiac troponin I second antibody, and cadmium sulfide is firmly bonded with mesoporous silica through disulfide bonds. After dithiothreitol is added dropwise, the disulfide bonds between cadmium sulfide and silica are disconnected, ascorbic acid is released, so that the photocurrent is improved to different degrees, and sensitive detection of cardiac troponin I is realized. The detection limit is 0.17pg / mL.

Description

technical field [0001] The invention relates to a method for preparing a photoelectrochemical immunosensor for detecting cardiac troponin I. Specifically, bismuth sulfide-sensitized tungsten trioxide is used as a base material, and mesoporous silica coated with ascorbic acid is encapsulated with cadmium sulfide The invention relates to the preparation of a split-type photoelectrochemical sensor for detecting cardiac troponin I by marking the secondary antibody of cardiac troponin I as a marker, which belongs to the technical field of new functional materials and biosensing detection. Background technique [0002] Cardiac troponin I (cTnI) has been used clinically for many years as a marker of myocardial injury. Due to its high myocardial specificity, high sensitivity to myocardial injury and long window period, cTnI has been widely accepted by clinicians and inspectors, and has not only become the "gold standard" for judging myocardial injury, especially acute myocardial inf...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30G01N33/531
CPCG01N27/305G01N27/3278G01N33/531
Inventor 魏琴徐芮范大伟吴丹曹伟马洪敏
Owner UNIV OF JINAN
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