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A kind of Maggi mech's yeast and its application

A technology of Mechi yeast and Maggi, applied in the field of microorganisms, can solve the problems of stable control effect without chemical fungicides, shallow depth, and not as easy to use as chemical reagents.

Active Publication Date: 2021-05-04
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reasons are as follows: 1) The research time of yeast is short and the depth is shallow. Most of the antagonistic yeasts reported in the literature are used to prevent and control the pathogenic bacteria in the wounds of postharvest fruits, rather than directly used on the fruit skin to prevent the invasion of pathogenic bacteria in normal fruits. ; 2) Under actual production conditions, yeast is greatly affected by the external environment, such as humidity, temperature and pH, which will have a great impact on the activity of yeast. Once the activity of yeast decreases, it will not be able to play a role in the disease. 3) Compared with chemical fungicides, the cost of using antagonistic yeasts is relatively high, and there is no stable control effect of chemical fungicides; 4) Yeasts cannot be stored at room temperature for a long time, and their service life is short, and they are not as good as chemical fungicides. Reagents are easy to use
A series of factors have affected the further development and utilization of antagonistic yeasts, and hindered the use of yeasts as fruit preservatives and biocontrol agents in production.

Method used

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  • A kind of Maggi mech's yeast and its application
  • A kind of Maggi mech's yeast and its application
  • A kind of Maggi mech's yeast and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Isolation and strain identification of Metschnikowia sp. T-2 of the present invention

[0046] 1. Sample collection

[0047] Fresh blueberries were purchased from Tianjin Hongqi Farmer's Fruit Wholesale Market.

[0048] 2. Isolation and screening of strains and antagonistic screening

[0049] Isolate strains from fresh blueberries, use conventional gradient dilution coating to separate, use YPD agar (containing ampicillin 25mg / L) medium to culture at 28°C, 180rpm, pick colonies with large differences in colony morphology and pure culture , purified and preserved on YPD agar medium (containing ampicillin 25mg / L), and carried out preliminary screening and multiple re-screening of antagonistic bacteria with blueberry postharvest Botrytis cinerea and Alternaria as target pathogens, and finally obtained a strain The yeast-like strain with strong antibacterial activity was named T-2.

[0050] 3. Identification of strains

[0051] Morphology and partial conserved sequences...

Embodiment 2

[0054] The low temperature tolerance test of Metschnikowia sp. T-2 of the present invention.

[0055] At present, the suitable growth temperature range of yeast (Metschnikowia sp.) reported in the literature is generally 20-30°C, and it cannot grow under the condition above 40°C. For example, the authorized patent "A Maggi Meki Yeast and Its Application" CN105349442B, the authorized patent "A Maggi Meki Yeast and Its Application for Inhibiting Fungi" CN 103642705, and the authorized patent "A Strain of Hansen Debariae hansenii strains and their application in "CN 104988082B" all the Debariae hansenii strains grow weakly at an ambient temperature of 4°C. However, Metschnikowia sp. T-2 described in this application grows well at 4°C, as shown in Figure 3(a), indicating that it is extremely resistant to low temperature and has extremely high low temperature tolerance .

[0056] The specific test method is as follows:

[0057] An equal amount of Metschnikowia sp. T-2 strain (1×...

Embodiment 3

[0059] Because blueberries belong to small berry fruits, and this type of fruit also includes grapes, strawberries, cherries, cherry tomatoes, etc., it has been proved by literature ([1] Li Ning. Detection of postharvest rot-causing bacteria in grapes and fungal elicitor-induced resistance [D]. Yang Ling, Northwest Agriculture and Forestry University 2005. [2] Reglinski T. Inhibition of Botrytis cinerea growth and suppression of botrytis bunch rot in grapes using chitosan [J]. Plant Pathology, 2010,59(5):882–890 .[3] Du Xiaoqin, He Jingliu, Qin Wen, et al. Research progress on the inhibitory effects of postharvest pathogenic bacteria and plant essential oils on sweet cherry fruits [J]. Food Industry Science and Technology, 2015(21): 368-371.[4] Alique R, Zamorano J P, Martínez M A, et al. Effect of hea and cold treatments on respiratory metabolism and shelf-life o sweet cherry, type picota cv “Ambrunés” [J]. Postharvest Biology and Technology, 2005, 35(2): 153-165.[5]Williamso...

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PUM

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Abstract

The present invention discloses a Maggi Mech's yeast T‑2, the storage number of which is: CGMCC No. 18253, and the application of the Maggi Mech's yeast in sterilization and preservation of fruit. Especially the application of the Maggi Metschniella in inhibiting and killing Botrytis cinerea and / or Alternaria. Fermenting the Maggi Mech's yeast to obtain a fermented liquid, the fermented liquid is used for sterilization and preservation of fruit. Fermenting the Maggi Metchi yeast to obtain its metabolites, which are used for sterilization and preservation of fruit; the metabolites are liquid metabolites or gaseous metabolites. The above-mentioned metabolites can also be used as a fungicide for fruit sterilization and preservation. This technology can not only be used as a fruit fungicide, but also has good safety, low temperature resistance, no side effects, simple operation and strong practicability.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Maggi meki yeast and its application. Background technique [0002] Blueberry (Vaccinium spp.), belonging to Rhododendronaceae, Vaccinium plant, is a perennial small shrub berry fruit tree, originated in North America. my country's blueberry production areas are mainly distributed in Liaoning, Heilongjiang, Jilin, Shandong coastal areas, Yunnan, Guizhou and other regions. Blueberry fruit contains a variety of nutrients, which can not only enhance eyesight and prevent eye fatigue, but also resist oxidation, anti-aging, lower blood pressure, lower cholesterol, etc., so it is called one of the five healthy fruits. In recent years, the scale of blueberry production in my country has gradually expanded. However, blueberries are berry fruits with high water content, thin peel and low hardness. Due to the concentrated picking period, which coincides with the high t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A23B7/155C12R1/645
CPCA23B7/155A23V2002/00C12N1/145C12R2001/645A23V2200/10A23V2250/76
Inventor 王远宏常若葵艾丹张斌田小卫
Owner TIANJIN AGRICULTURE COLLEGE
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