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Method for sterile propagation of solanum torvum stocks through test tube micro-cutting

A technology of micro cuttings and rootstocks, applied in seed immunization, horticultural methods, seed and rhizome treatment, etc., can solve the problems of low germination rate, germination potential and germination index, slow seedling growth speed, and limited scale application, etc., to meet the needs of large The effects of large-scale seedling breeding, high survival after transplanting, and easy operation

Inactive Publication Date: 2019-12-06
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the long age of eggplant seedlings, low germination rate, germination potential and germination index, and slow growth of seedlings, it has limited its large-scale application in industrial seedling cultivation. cost

Method used

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  • Method for sterile propagation of solanum torvum stocks through test tube micro-cutting
  • Method for sterile propagation of solanum torvum stocks through test tube micro-cutting
  • Method for sterile propagation of solanum torvum stocks through test tube micro-cutting

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Experimental program
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Effect test

Embodiment 1

[0029] A method for aseptic propagation of eggplant rootstock test tube micro-cutting, comprising the following steps:

[0030] Step 1: Culture medium preparation, bud differentiation medium with MS medium plus KT0.5mg / L, IBA0.10 mg / L, sucrose 30g / L, agar 4.8g / L; subculture medium with MS medium IBA0.40mg / L, sucrose 30g / L, agar 4.5-5.0g / L; rooting medium is 1 / 2MS medium supplemented with IBA0.20 mg / L, sucrose 30g / L, agar 4.5-5.0g / L. Adjust the pH of the above medium to 5.8, put 50mL of each bottle into tissue culture bottles, seal it and put it into an autoclave for sterilization at 121°C for 20 minutes;

[0031] Step 2: To obtain aseptic seedlings, soak the eggplant seeds in 1g / L gibberellin for 3 hours, make the seeds swell completely, then transfer them to the ultra-clean workbench for disinfection, first rinse with 70% ethanol for 15 seconds, and rinse with sterilized water After 3 times with 0.1% HgCl 2 Disinfect for 10 minutes, rinse with sterilized water for 5 times, ...

Embodiment 2

[0039]Step 1: Culture medium preparation, bud induction medium is supplemented with MS medium KT0.4mg / L, IBA0.05 mg / L, sucrose 25g / L, agar 4.5g / L; subculture proliferation medium is supplemented with MS medium IBA0.15mg / L, sucrose 30g / L, agar 4.5g / L; rooting medium is 1 / 2MS medium supplemented with IBA0.15mg / L, sucrose 30g / L, agar 4.8g / L. Adjust the pH of the above medium to 5.8, put 50mL of each bottle into tissue culture bottles, seal it and put it into an autoclave for sterilization at 121°C for 20 minutes;

[0040] Step 2: To obtain aseptic seedlings, soak the eggplant seeds in 1g / L gibberellin for 3 hours, make the seeds swell completely, then transfer them to the ultra-clean workbench for disinfection, first rinse with 70% ethanol for 15 seconds, and rinse with sterilized water After 3 times with 0.1% HgCl 2 Disinfect for 10 minutes, rinse with sterilized water for 5 times, blot the surface moisture with sterilized filter paper, and carefully sow the seeds on the surfac...

Embodiment 3

[0047] Step 1: Culture medium preparation, bud induction medium is supplemented with MS medium KT0.45mg / L, IBA0.15 mg / L, sucrose 32g / L, agar 5.0g / L; subculture proliferation medium is supplemented with MS medium IBA0.25mg / L, sucrose 32g / L, agar 5.0g / L; rooting medium is 1 / 2MS medium supplemented with IBA0.25mg / L, sucrose 32g / L, agar 5.0g / L. Adjust the pH of the above medium to 6.0, put 50mL of each bottle into tissue culture bottles, seal it and put it into an autoclave for sterilization at 121°C for 20 minutes;

[0048] Step 2: To obtain aseptic seedlings, soak the eggplant seeds in 1g / L gibberellin for 3 hours, make the seeds swell completely, then transfer them to the ultra-clean workbench for disinfection, first rinse with 70% ethanol for 15 seconds, and rinse with sterilized water After 3 times with 0.1% HgCl 2 Disinfect for 10 minutes, rinse with sterilized water for 5 times, blot the surface moisture with sterilized filter paper, and carefully sow the seeds on the surf...

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Abstract

The invention discloses a method for sterile propagation of solanum torvum stocks through test tube micro-cutting. The method comprises the steps of obtaining of aseptic seedlings, bud induction culture, multiplication subculture, rooting culture and seedling hardening and transplanting. A bud induction culture medium is prepared from MS, 0.4-0.6 mg / L KT and 0.05-0.15 mg / L IBA, a subculture multiplication culture medium is prepared from MS and 0.35-0.45 mg / L IBA, and a rooting culture medium is prepared from 1 / 2 MS and 0.15-0.25 mg / L IBA. According to the method, a stem segment is taken as anexplant, and the explant is simple and easy to take; matching of a plant growth regulator is simple, a used reagent is low in price, and the cost is low; the bud induction rate reaches 90%, and after15 days, lateral buds of about 1 centimeter can grow; subculture multiplication is carried out for 30 days, the multiplication coefficient reaches 5-6 times, the rooting effect is significant, and thetransplanting survival rate is high. The method for sterile propagation of solanum torvum stocks through test tube micro-cutting has the advantages that a whole tissue culture rapid propagation technology system of solanum torvum is established, the whole production process cycle is short, the operation is simple and convenient, the proliferation multiple is high, the large-scale seedling propagation demands can be met completely, and the method has good application prospects.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture rapid propagation, in particular to a method for aseptic propagation of eggplant rootstock test tube micro-cutting. Background technique [0002] Solanum torvum, also known as "Torubum", is a wild relative of the cultivated eggplant (Solanum melogena), native to America. The plant is highly resistant to major soil-borne diseases and insect pests such as bacterial wilt, verticillium wilt, fusarium wilt, and root-knot nematode, and is a grafted rootstock commonly used in eggplant and tomato production. [0003] Although the growth potential of the plant is extremely strong, the eggplant seed grains are extremely small and the endogenous hormone content is low, so the seeds have strong dormancy after maturity, and it takes about one month for the eggplant seeds to germinate normally. After sprouting and breaking through the soil, the seedlings grow very slowly at the initial stage, espe...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00A01C1/00A01C1/08
CPCA01C1/00A01C1/08A01G31/00A01H4/001A01H4/008
Inventor 于文进张映卿钟川阳燕娟
Owner GUANGXI UNIV
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