A fusiform lysine Bacillus ca1 and its application in promoting the growth of pocket mushrooms
A lysine bacillus and spindle-shaped technology is applied to the spindle-shaped bacillus lysine CA1 and its application field in promoting the growth of pocket mushrooms, and can solve the problem of low growth promoting efficiency, insignificant effect and environmental pollution of chemical fertilizers and other problems, so as to improve the economic and social benefits of production, reduce the amount of auxiliary materials, and increase the fruiting yield.
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Embodiment 1
[0024] 1) Sample collection and storage
[0025] Soil samples of pocket mushroom fruiting bodies, fungus bags, and soil-covered edible fungi were randomly collected in Jining Changshengyuan Mushroom Industry Co., Ltd., and the samples were taken by aseptic operation. After sampling, they were put into sterile bags and stored at -20°C.
[0026] 2) Purification and preservation of bacteria
[0027] Accurately weigh 1g of the soil sample to be tested, put it into a sterilized test tube filled with 9mL of sterile water, place it on a shaker at 28°C, 170r / min, and shake for 20min to disperse the microbial cells, and let it stand for 20-30s. into 10 -1 diluent; then use a 1mL sterile pipette to draw 10 -1 Dilute 1mL, transfer it into a test tube filled with 9mL sterile water, blow and aspirate the bacterial solution to mix evenly, and serve as 10 -2 Diluent; change another sterile pipette to draw 10 -2 Dilute 1mL, transfer to a test tube filled with 9mL sterile water, blow and a...
Embodiment 2
[0046] 1) Preparation of bacterial agent
[0047] Inoculate the activated bacterial strain of Bacillus lysinica fusiformis CA1 on the seed expansion medium (starch 6~20g / L, K 2 HPO 3 4~12g / L, ammonium sulfate 2~5.5g / L, yeast extract 0.5~5g / L, sucrose 4~10g / L, MgSO 4 2~5g / L, CaCO 3 2~5g / L, SiO 2 2~10g / L, FeCl 3 0.02~1g / L, pH 7.2~7.4), cultivated under the conditions of 28~32℃, 150~200r / min for 18~24h, to obtain the seed solution;
[0048] The obtained seed liquid is inoculated in the fermentation medium (sucrose 2~5g / L, K 2 HPO 3 2~5g / L, yeast extract 1.2~5g / L, ammonium sulfate 0.2~5g / L, soybean peptone 1.5~5g / L, MgSO 4 0.4~5g / L, pH7.2~7.4), the inoculum amount is 5~10% (volume percentage), cultivated for 48~72h at 28~32℃ and the stirring speed of the fermenter is controlled at 150~200r / min , control the ventilation rate 1:0.5-2vvm, and obtain the fermentation broth. The effective number of lysine bacillus in the fermentation broth is 1.0×10 9 cfu / mL~2.0×10 9 ...
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