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Metschnikowia pulcherrima strain for preventing and treating grey speck disease after loquat harvest and application thereof

A gray spot and yeast technology, applied in the field of Maggi Mech's yeast E1, can solve the problems of high cost of prevention and treatment, high chemical pesticide residues, etc., and achieve the effects of strong disease-causing effect, reduced losses, and significant economic and social benefits

Active Publication Date: 2020-01-14
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems of high cost of loquat postharvest fungal disease control and high chemical pesticide residues in the current agricultural production, the present invention isolates and identifies a dominant pathogen of loquat gray spot disease in Zhenjiang area from postharvest rotten loquats, and provides a strain isolated from loquat at the same time. Metschnikowia pulcherrima E1 from loquat branches and leaves can significantly reduce the incidence of loquat postharvest gray spot disease and the diameter of the lesion, and reduce the loss caused by postharvest rot of loquat, which has potential application value

Method used

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  • Metschnikowia pulcherrima strain for preventing and treating grey speck disease after loquat harvest and application thereof
  • Metschnikowia pulcherrima strain for preventing and treating grey speck disease after loquat harvest and application thereof
  • Metschnikowia pulcherrima strain for preventing and treating grey speck disease after loquat harvest and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Isolation, purification and identification of pathogenic bacteria

[0029] 1.1 Isolation and purification of pathogenic bacteria

[0030] The organic loquat fruits of the "Bahong" variety, which are of the same maturity and size and free from diseases and insect pests, were picked from the "Yunbiao Orchard" in Yangzhong, Zhenjiang City, Jiangsu Province. They were transported back to the laboratory and placed in plastic baskets sterilized by sodium hypochlorite. Seal and store at room temperature for about 15 days, collect fruits with obvious symptoms, and use tissue separation method to isolate pathogenic bacteria. Use a sterile scalpel to cut out a tissue block with a size of about 5 mm from the rotten part, disinfect it with 75% ethanol for a few seconds, rinse it with sterile water 3 times, drain it, place it on a PDA medium, and culture it in a constant temperature incubator at 28°C . After the colony grows, pick the hyphae at the edge of the colony...

Embodiment 2

[0039] Example 2: Isolation, screening and identification of antagonistic yeast strains

[0040] 2.1 Isolation and purification of antagonistic yeast

[0041] Take samples of loquat branches and leaves from "Yunbiao Orchard" in Yangzhong, Zhenjiang City, Jiangsu Province, cut them into pieces with a size of 1 cm, put them into a conical flask (250 mL) containing 90 mL of PDB medium, and add a few drops of streptomycin sulfate Solution (about 3 mg), cultured at 28°C, 180rpm constant temperature shaking for 20h. The bacterial solution was diluted to 10 with sterile water using a ten-fold gradient dilution method. -4 、10 -5 、10 -6 , pipette 100 μL of the diluted solution and spread it on a Bengal red medium plate, and incubate at a constant temperature of 28°C for 2 to 3 days. Observe the colony morphology of each yeast strain, and then pick out single colonies with different culture characteristics and purify them on NYDA medium plates. The isolated and purified strains wer...

Embodiment 3

[0051] Example 3: Safety study on Metschnikowia pulcherrima E1

[0052] The tested animal species were ICR mice, provided by the Experimental Animal Center of Jiangsu University. A total of 40 clean-grade mice weighing 18-22 g were selected, half male and half male. In the test group, there were 10 male and female mice each, and the maximum limit method was adopted according to the national standard, and the test dose was 10000 mg / kg. Oral gavage was adopted, according to the test of 0.2mL / 10g body weight, and the gavage was continued for 14 days; the same 10 male and female mice in the control group were gavaged with sterile water instead of yeast suspension. Throughout the experiment, the mice were observed every day for their activity, food intake, body weight changes, and whether there was any poisoning or death.

[0053] According to the above test procedures, the statistical results of the safety test of Metschnikowia pulcherrima E1 are as follows: the mice injected wi...

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Abstract

The invention discloses a Metschnikowia pulcherrima strain for preventing and treating grey speck disease after loquat harvest and an application thereof, which belongs to the technical field of postharvest biological prevention and treatment of fruits. The biocontrol saccharomycetes is identified as Metschnikowia pulcherrima, and a preservation number is CCTCC M 2019256. The preparation method comprises the following steps: firstly, preparing a Pestalotiopsis vismiae P2 conidiospore suspension and a Metschnikowia pulcherrima E1 bacterial suspension for later use; punching a hole (3mm*3mm) inan equator part of the loquat fruit, adding the E1 bacterial suspension, and adding P2 conidiospore suspension after 2 hours; and airing, putting the material into a plastic basket, sealing with a preservative film, and storing the material at room temperature. The morbidity and rotting direct drop of the loquat grey speck disease treated by the bacterial suspension are obviously reduced. Therefore, the Metschnikowia pulcherrima can be used for preventing and treating the grey speck disease after loquat harvest, and the harm to human health and the pollution to the environment caused by the use of chemical sterilization are reduced.

Description

technical field [0001] The invention relates to a strain of Metschnikowia pulcherrima E1, which can control loquat postharvest gray spot disease, and belongs to the technical field of fruit postharvest biological control. Background technique [0002] Loquat (Eriobotrya japonica. IAndl) belongs to Rosaceae (Rosaceae), loquat (Eriobotrya), native to southeastern China, the mature loquat fruit tastes sweet, rich in nutrition, rich in fructose, glucose, carotene and vitamins A and B , C and other ingredients, and has the effects of moistening the lungs, relieving cough and quenching thirst, and is deeply loved by consumers. At present, the planting area and output of loquat in our country are increasing rapidly, and the production scale has accounted for more than 80% of the world's loquat. It has an absolute advantage in the world and has become one of my country's most competitive fruits in the international market. Due to the thin peel and tender and juicy flesh of loquat, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/14A23B7/155C12R1/645
CPCC12N1/16A23B7/155C12R2001/645C12N1/145
Inventor 孙卫红杨慧慧王玲赵一繁李胜杰毋宁宁高兴彪
Owner JIANGSU UNIV
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