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Fusarium aromatic fungus hnu066 and its application in the degradation of waste gas containing hydrogen sulfide

A technology of Fusarium and fungus, applied in the field of environmental pollution control, can solve the problems of short effective time, difficult preservation, high maintenance cost, etc., and achieve the effects of good storability, shortened domestication time, and improved removal efficiency

Active Publication Date: 2021-07-27
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current bio-trickling filter reactors have disadvantages such as low efficiency, short effective time, difficult storage and high maintenance costs, which limit the application of bio-trickling filter reactors in the field of exhaust gas purification to a certain extent.

Method used

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  • Fusarium aromatic fungus hnu066 and its application in the degradation of waste gas containing hydrogen sulfide
  • Fusarium aromatic fungus hnu066 and its application in the degradation of waste gas containing hydrogen sulfide
  • Fusarium aromatic fungus hnu066 and its application in the degradation of waste gas containing hydrogen sulfide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Screening, isolation, and culture of Fusarium aromatica fungus "HNU066"

[0031] Aromatic Fusarium fungus "HNU066" was isolated from Rousselot (Wenzhou) Gelatin Co., Ltd. bioreactor ( figure 1 ). The separation method is as follows: take 5 g of the acclimated biological trickling tower reactor sludge, dilute it with distilled water to three different concentration gradients of 10 times, 100 times and 1000 times, and spread on the PDA fungal solid medium respectively. Each petri dish was coated with one milliliter of the diluted sludge leaching solution, incubated in the dark at a constant temperature of 25 degrees, and the single colony formed was observed after culturing for one week. Pick the mycelium from the edge of the selected single colony, transfer it to a fresh PDA plate for streak separation and culture, and repeat the streak operation until a pure culture is obtained, which is transferred to a PDA slant for storage.

Embodiment 2

[0032] Example 2: Morphological and molecular biological identification of Fusarium aromatica fungus "HNU066"

[0033] Under the microscope, observe the characteristics of the colony, hyphae and spores of the endophytic fungi, and refer to the "Fungus Identification Manual" for identification. Specifically, the screened Fusarium aromatica fungus "HNU066" strain was seeded on a PDA plate by the spot method, placed at a constant temperature of 25 degrees for cultivation, and the morphological characteristics of the strain were observed with the naked eye, including colony morphology, color, size, Edge characteristics, mycelial traits, growth rate and other characteristics. The hyphae on the surface of the colony were picked and placed under an optical microscope (Japan, OLYMPUS) to observe the presence or absence of spores and spore-forming structures.

[0034] The morphological characteristics of the aromatic Fusarium fungus "HNU066" of the present invention are as follows:

[...

Embodiment 3

[0036] Example 3: Molecular biological identification of Fusarium aromatica fungus "HNU066"

[0037] The genomic DNA of Fusarium aromaticum fungus "HNU066" was extracted by the general fungal DNA extraction method. The extraction method is as follows: take 1.5 mL of bacterial liquid, 12000 × g, 2 min, collect the bacterial cells; add 500 μL of 5 × CTAB (containing 1% β-mercaptoethanol), quick-freeze in liquid nitrogen for 30 s, and then transfer to 65 degrees for a warm bath for 30 s, Repeat the above process 3 times; vortex at high speed for 3-5min, warm bath at 65°C for 20min, add 2ml of phenol:chloroform:isoamyl alcohol (25:24:1, V / V / V), 12000×g, 10min, take the supernatant; add 2-fold volume of absolute ethanol to the supernatant, and centrifuge again after standing for 20 minutes; pour off the supernatant and wash the precipitate with 70% alcohol; dry under natural conditions to obtain fungal DNA for use.

[0038] Using the fungal universal primers ITS5 (5'-GGAAGTAAAAGTC...

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Abstract

The invention discloses the aromatic fusarium fungus HNU066 and its application in the degradation of waste gas containing hydrogen sulfide. The fungal strain "HNU066" of the genus Fusarium redolens of the present invention has a preservation number: CCTCC NO: M 2019500. Application of fungus HNU066 in the degradation of exhaust gas containing hydrogen sulfide. The fungus "HNU066" increases its abundance in the active packing of the biotrickling filter reactor, which can improve the removal efficiency of the bioactive packing for hydrogen sulfide-containing waste gas. Adding aromatic Fusarium fungus "HNU066" to the reactor, after entering the stable period, the absorption and removal efficiency of hydrogen sulfide-containing waste gas increased by 34.5% compared with the control group.

Description

technical field [0001] The invention belongs to the field of environmental pollution control, and relates to a sulphur fungus HNU066 of the genus Fusarium redolens and its application in the biodegradation of industrial waste gas containing hydrogen sulphide. Background technique [0002] With the intensification of environmental pollution, industrial waste gas emissions have become a major culprit affecting people's healthy life. In the actual industrial waste gas emissions, the waste gas containing hydrogen sulfide occupies an important proportion, so it is listed as one of the national priority control gas pollutants. Hydrogen sulfide is a foul-smelling gas with the smell of rotten eggs, which seriously affects the life of surrounding residents. Therefore, the discharge of a large amount of hydrogen sulfide-containing waste gas seriously damages human health. In addition, the formation of sulfate ions after the hydrogen sulfide discharged into the atmosphere is the main ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14B01D53/84B01D53/52C12R1/77
CPCB01D53/52B01D53/84B01D2251/95C12N1/14C12N1/145C12R2001/77Y02A50/20
Inventor 刘奇沈晨佳倪建国成卓韦宋欣欣杨华云李伟东
Owner HANGZHOU NORMAL UNIVERSITY
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