Preparation method and application of stem cell supernate rich in VEGF and FGF

Abstract

The invention discloses a preparation method and an application of a stem cell supernate rich in VEGF and FGF. The preparation method comprises the following steps: preparing umbilical cord stem cells, over-expressing human the vascular endothelial cell growth factor (VEGF) and the human fibroblast growth factor (FGF) through a molecular cloning technology, then carrying out amplification passage,and collecting the obtained cell culture supernate to obtain the finished stem cell supernate. The supernate can be applied to repair treatment of diabetic foot gangrene wounds with limb ischemia. The preparation method has the advantages o reasonable process design, simplicity in operation, and high feasibility, and the obtained supernate contains the over-expressed VEGF and FGF, also contains human epidermal cell growth factors, human keratinocyte growth factors, human platelet-like derivative growth factors, fibronectin, immune factors and other factors, can be applied to repair treatmentof diabetic foot extremity ischemia, provides a new way for application of the stem cells to medical transformation, and has high practicability.

Description

technical field [0001] The invention relates to the technical field of biology, in particular to a preparation method and application of stem cell supernatant rich in VEGF and FGF. Background technique [0002] According to statistics from the International Diabetes Federation, the number of diabetic patients in my country reached 114 million in 2018, and the number of adult patients worldwide was 415 million. It is estimated that the number of diabetic patients worldwide will rise to 620 million by 2040. Diabetic foot is one of the chronic complications of diabetes that is difficult to treat, and severe cases require amputation. At present, the commonly used clinical treatment methods for diabetic foot include lowering blood sugar, repeated wound cleaning, preventing inflammation from worsening, improving blood supply of lower limbs, continuous negative pressure suction, hyperbaric oxygen therapy, etc. Lower extremity arterial intervention or lower extremity artery bypass ...

AI Technical Summary

Problems solved by technology

At present, the commonly used clinical treatment methods for diabetic foot include lowering blood sugar, repeated wound cleaning, preventing inflammation from worsening, improving blood supply of lower limbs, continuous negative pressure suction, hyperbaric oxygen therapy, etc. Lower extremity arterial intervention or lower extremity artery bypass grafting, but for diabetic foot patients with severe microvascular complications and macrovascular complications, the effect of surgery to improve vascular circulation is not satisfactory, and the probability of amputation is still high

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