Photosynthetic bacterium with high yield nano-selenium and preparation method of nano-selenium living bacterium preparation thereof

A technology for photosynthetic bacteria and live bacteria preparations, applied in the fields of botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc. problems, to achieve the effect of low cost, low equipment requirements, and no three wastes discharge

Pending Publication Date: 2020-04-07
FOSHAN YANHUI BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem solved by the present invention is that the existing microorganisms are more resistant to the low concentration of sodium selenite and the low conversion rate of nano-selenium. More importantly, the existing nano-selenium production technology will basically be purified by centrifugation, and there will be waste water discharge, and high-concentration liquid biological nano-selenium is unstable in physical and chemical properties in long-term storage, and the content of viable bacteria is low, which limits its application and production

Method used

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  • Photosynthetic bacterium with high yield nano-selenium and preparation method of nano-selenium living bacterium preparation thereof
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  • Photosynthetic bacterium with high yield nano-selenium and preparation method of nano-selenium living bacterium preparation thereof

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Effect test

Embodiment 1

[0035] The screening of embodiment 1 biological nano-selenium producing bacteria

[0036] Preliminary screening: 115 strains of Bacillus, 32 strains of lactic acid bacteria, and 22 strains of photosynthetic bacteria commonly used in the laboratory of the inventor's company were cultivated in a selective medium containing sodium selenite. The red color of the bacterial liquid indicates the presence of nano The production of selenium, preliminary screen out can reduce sodium selenite to produce nano selenium strain.

[0037] The initial screening process is as follows:

[0038] Bacillus: in a sterilized 500 milliliter Erlenmeyer flask, add in the LB liquid culture medium containing 0.5g / L sodium selenite,

[0039] At the same time, according to the 1% (v / v) inoculum amount, the activated bacterial seed solution was added sequentially, and the culture was shaken at 30°C and 180 r / min for 2d-5d.

[0040] Lactic acid bacteria: in 500 milliliters of sterilized Erlenmeyer flasks,...

Embodiment 2

[0049] Embodiment 2 investigates the impact of different concentrations of sodium selenite on JCD-1 bacteria and the conversion rate of nano-selenium

[0050] Investigate the influence of different concentrations of sodium selenite on JCD-1 Sulfur japonica and the conversion rate of nano-selenium, using the photosynthetic bacteria liquid culture medium in Example 1, only changing the concentration of sodium selenite. Selenium content in all samples was determined using flow injection hydride-atomic absorption spectrometry. The culture solution containing the bacteria was taken at 4°C and centrifuged at 10000rpm to measure the selenium content in the supernatant. Conversion rate=(A-B) / A×100%. In the formula, A is the initial selenium content in the photosynthetic bacteria medium; B is the remaining selenium content in the medium after cultivation.

[0051] It can be seen from Table 3 that at a lower concentration (0.5-2g / L), as the concentration of sodium selenite increases, ...

Embodiment 3

[0053] Embodiment 3 investigates the stability test of different materials to nano selenium of the present invention

[0054] Because our company is in Guangdong area, the part of nano-selenium fermented liquid that produces is in summer, and its room temperature storage is no more than 30 days, just occurs the situation of black selenium, therefore carried out following test, JCD-1 bacterium is in 2g / L sub- After 7 days of fermentation in photosynthetic bacteria medium with sodium selenate (11.567mmol / L) concentration, the conversion rate was detected to be 95.5%, and the number of viable bacteria was 2.95 billion. Take 500mL each and place them in a triangular flask, and add 46.28mmol / L of Various amino acids (partial amino acid solubility is too low, such as tyrosine uses its hydrochloride), niacin, 12g / L soybean protein isolate, 12g / L sucrose, 12g / L milk powder, 12g / L poly grape to adjust pH Up to 7.0, three groups of parallel experiments, in a 45°C incubator (in order to ...

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Abstract

The invention discloses a photosynthetic bacterium with high yield nano-selenium and a preparation method of a nano-selenium living bacterium preparation thereof. The invention particularly disclosesthiocapsa roseopersicina capable of tolerating high-concentration (25g/L) sodium selenite. Nicotinic acid is creatively used for modifying high-concentration biological nano-selenium, so that the storage stability of the nano-selenium in the liquid is greatly improved. Meanwhile, the conversion rate of the nano-selenium is over 90 and thus nano-selenium fermentation liquid can be not subjected tocentrifugal purification, so that a liquid nano-selenium preparation which is relatively high in nano-selenium content, contains viable bacteria and is long in preservation time can be directly obtained. Problems that high-concentration biological nano-selenium prepared by the microorganisms in the prior art can not be stored in a liquid mode and easy transformation to black elemental selenium occurs frequently are solved and defects of high energy consumption and serious pollution are overcome. The nano-selenium living bacterium preparation has advantages of simple production process, low cost, low equipment requirement, no emission of three wastes, and environmental friendliness has the great market popularization value.

Description

technical field [0001] The invention relates to the field of nano-biological materials, in particular to a photosynthetic bacterium with high yield of nano-selenium and a photosynthetic bacterium that does not discharge three wastes, contains high-concentration nano-selenium, has a high conversion rate of nano-selenium, and has a high content of viable bacteria, and can be stored stably for a long time A preparation method of a liquid nano-selenium preparation. Background technique [0002] Selenium is an essential trace element for the human body. As a nutrient element, selenium can remove excess active oxygen free radicals in the human body and participate in the synthesis of 25 kinds of selenoproteins, such as glutathione peroxidase. The occurrence of many diseases is related to selenium deficiency. People have been looking for safe and economical ways to supplement selenium nutrition. The most common way is to supplement selenium-enriched agricultural products. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C12R1/01
CPCC12N1/20A01N63/00C12R2001/01C12N1/205
Inventor 蒋常德蒋常银张国睿
Owner FOSHAN YANHUI BIOTECH CO LTD
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