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Heterotrophic nitrification and aerobic denitrification bacterium and application thereof in pig raising wastewater treatment

An aerobic denitrifying bacteria and heterotrophic nitrification technology, applied in the field of environmental microorganisms, can solve the problems of increased investment cost, low biomass concentration, slow growth, etc., and achieve the effects of good application value, simple operation and mild reaction.

Active Publication Date: 2020-05-01
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the nitrogen in the water body exceeds the nitrogen required to meet the requirements of fish farming, it usually leads to excessive growth of algae, covering the water surface, resulting in insufficient dissolved oxygen in the water body, and the death of aquatic organisms due to oxygen depletion
(2) Harmful to human health
(3) Increase the cost of sewage treatment
However, it has the following disadvantages: (1) Nitrifying bacteria are inorganic chemical energy autotrophic bacteria, and the type of nutrient metabolism determines their slow growth, long generation cycle, low biomass concentration, poor environmental adaptability, weak impact load resistance, and high concentration of ammonia nitrogen. and nitrite nitrogen are more likely to inhibit its growth
(2) The two reactions of nitrification and denitrification cannot be unified in time and space, which increases the investment cost
However, most of these related reports are reports on the denitrification characteristics of the strains. For the heterotrophic nitrifying aerobic denitrifying bacteria with high concentration of ammonia nitrogen removal, heavy metal resistance, salt tolerance, and simultaneous removal of COD and ammonia nitrogen from swine wastewater Delftia tsuruhatensis has not been reported

Method used

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  • Heterotrophic nitrification and aerobic denitrification bacterium and application thereof in pig raising wastewater treatment
  • Heterotrophic nitrification and aerobic denitrification bacterium and application thereof in pig raising wastewater treatment
  • Heterotrophic nitrification and aerobic denitrification bacterium and application thereof in pig raising wastewater treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Screening and isolation and identification of heterotrophic nitrification-aerobic denitrification strains

[0059] Take samples from the pig farm manure composting fermentation tank in Zhubian Town, Junan County, Linyi City, Shandong Province, and mix 5 g of the compost samples into 45 mL of sterilized VM enrichment medium.

[0060] Then placed at 30°C, 150rpm constant temperature shaker for enrichment culture for 12h. After gradient dilution of the enriched solution, evenly spread on VM separation medium (agar 20g / L, other components are the same as VM). After culturing in a constant temperature incubator at 30°C for 1 day, single clones with different shapes and sizes were picked, streaked and purified, and numbered and stored to obtain the strains for primary screening. Put the isolated strains into 300ml wide-mouth Erlenmeyer flasks containing 100ml of nitrifying medium, place them in a shaker at 30°C and 150rpm for 16 hours, then take the bacterial liqui...

Embodiment 2

[0066] Example 2 Analysis of Influencing Factors for the Removal of Ammonia Nitrogen by Delftia tsuruhatensis SDU2

[0067] 1. The effect of carbon source on the removal of ammonia nitrogen by Delftia tsuruhatensis SDU2

[0068] Glucose, sucrose, methanol, glycerol, sodium acetate, sodium citrate, and sodium succinate were selected as the sole carbon source of the nitration medium, and the carbon-nitrogen ratio in the medium was C / N=10. The activated bacterial liquid in the LB medium was inserted into the nitrification medium according to the inoculum amount of 10%, and after culturing for 32 hours at 30°C and 150rpm, the calculation was carried out according to the implementation case 1, and the results were as follows figure 2 shown. The results show that the ammonia nitrogen removal rate of Delftia tsuruhatensis SDU2 using sodium acetate, sodium succinate and sodium citrate can reach more than 60%, and the ammonia nitrogen removal rate of sodium citrate can reach more tha...

Embodiment 3

[0083] Embodiment 3 is suitable for (Delftia tsuruhatensis) SDU2 ammonia nitrogen removes optimal culture medium and optimal culture condition

[0084] Based on the screening results of Example 2, determine the optimal medium suitable for Crane Haneda Delftia (Delftia tsuruhatensis) SDU2: 4.07g sodium citrate; 0.47g (NH 4 ) 2 SO 4 ; 7.9 g Na 2 HPO 4 ·7H 2 O; 1.5 g KH 2 PO 4 ,0.1g MgSO 4 ·7H 2 O and 2mL trace element solution; make up to 1L with distilled water. Among them, trace element solution: 50.0gNa 2 EDTA; 2.2 g ZnSO 4 ·7H 2 O; 5.5 g CaCl 2 ; 5.06g MnCl 2 4H 2 O; 5.0 g FeSO 4 ; 1.57g CuSO 4 ·5H 2 O; 1.60g CoCl 2 ·6H 2 O; distilled water to 1L.

[0085]Optimal culture conditions suitable for Delftia tsuruhatensis SDU2: 30° C., 150 rpm, pH=7, cultured for 168 hours, and the removal rate of ammonia nitrogen is over 90%.

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Abstract

The invention discloses a heterotrophic nitrification and aerobic denitrification bacterium, which is named as (Delftia tsuhatensis) SDU2 and is preserved in the General Microbiological Center of theChina Committee for Culture Collection of Microorganisms (CGMCC). The preservation number of the strain is CGMCC No.19313. The invention also discloses an application of the heterotrophic nitrification and aerobic denitrification bacterium in pig raising wastewater treatment. Experiments prove that the strain disclosed by the invention can tolerate ammonia nitrogen wastewater with the concentration as high as 2000ppm, can tolerate 3.5% of salt concentration, has tolerance to 20ppm metal ions Cu2+, Zn2+; Mn2+; and Ni2+ respectively, and has tolerance to 5ppm Gr6+; in addition, under the condition that the carbon-nitrogen ratio of the pig raising wastewater is maintained to be about 10 by additionally adding a carbon source sodium citrate, the strain disclosed by the invention has a relatively high removal rate on ammonia nitrogen and COD in the wastewater, and has a very good application value in a culture wastewater treatment project.

Description

technical field [0001] The invention relates to a heterotrophic nitrifying aerobic denitrifying bacterium and its application, in particular to a heterotrophic nitrifying aerobic denitrifying bacterium and its application in pig wastewater treatment. It belongs to the technical field of environmental microorganisms. Background technique [0002] With the rapid development of industrial and agricultural production and people's increasing living standards, the discharge of nitrogen-containing compounds has increased sharply, which has become a major source of environmental pollution and has attracted widespread attention from all walks of life. When there is too much nitrogen in the water body, the harm to the environment and organisms is mainly manifested in the following aspects: (1) Destruction of the water body ecosystem. When the nitrogen in the water body exceeds the nitrogen required to meet the requirements of fish farming, it usually leads to excessive growth of alga...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/20
CPCC12N1/20C02F3/34C02F2103/20C12R2001/01C12N1/205
Inventor 林建强陈利飞陈林旭潘登任一林林慧彬林建群
Owner SHANDONG UNIV