Streptococcus and application thereof

A technology of streptococcus and microbial strains, applied in the field of microorganisms, can solve the problem of rare probiotic products, and achieve the effect of great application potential

Active Publication Date: 2020-05-05
SHENYANG MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing probiotic products are mainly aimed at the regulation and control of

Method used

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  • Streptococcus and application thereof
  • Streptococcus and application thereof
  • Streptococcus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Isolation and identification of symcsis 3-17 streptococci.

[0028] 1. Isolation and culture of symcsis 3-17 streptococci.

[0029] symcsis 3-17 is isolated from throat swabs of healthy children, separated and purified on nutrient agar containing 5% (v / v) defibrated sheep blood, and picks 0.5-1.0mm of grass-green hemolysis ring on the pick plate single colony in diameter. The colonies were inoculated on nutrient blood agar, cultured under aerobic conditions at 37°C for 24 hours, and independent colonies were selected from the culture dish for subculture and purification.

[0030] Screening of culture conditions: culture on agar plate containing 5% defibrated sheep blood for 24 hours, set culture temperature as 4, 15, 22, 25, 30, 35, 37, 42, 45°C, aerobic, anaerobic and Under the micro-aerobic environment, the growth condition of the bacterial strain is tested; under the presence of adding 2.5, 3.5, 4.5 or 6.5% (g / L) NaCl to the culture medium, the salt toler...

Embodiment 3

[0071] Bacteriostatic experiment of embodiment 3 symcsis 3-17 streptococci.

[0072] 1. Enrichment of symcsis 3-17 streptococci.

[0073] Pick a single colony of symcsis 3-17 Streptococcus and inoculate it on a sheep blood agar plate, culture it at 37°C for 24 hours, then further enrich and amplify pure Streptococcus on a sheep blood agar plate.

[0074] 2. Determination of symcsis 3-17 streptococcal antagonism and long-term antagonism.

[0075] Pick single colonies 3-17 grown on the blood agar plate, culture in BHI medium containing 5% fetal bovine serum for 24 hours, fully vortex, and dilute to 10 8 cfu / mL, spare. will be diluted to 10 6 100ul of cfu / mL pathogenic bacteria suspension were spread on BHI agar plates respectively. Place the Oxford cup, draw 200ul3-17 streptococcus into the Oxford cup, incubate at 37°C for 24 hours, observe the antibacterial situation, and measure the antibacterial diameter. Streptococcus 3-17 was subcultured once a day, and antagonistic te...

Embodiment 4

[0077] Example 4 Antibiotic susceptibility test of symcsis 3-17 streptococci.

[0078] Using the disk diffusion method, according to the method of CLSI (Clinical Laboratory Standards Institute), the test includes clindamycin DA2ug, erythromycin E15ug, linezolid LZD30ug, chloramphenicol C 30ug, cefotaxime CTX 30ug, penicillin G P10 10units, vancomycin VA30ug, ampicillin AMP10 10ug, ceftriaxone CRO30ug, cefepime FEP 30ug (OXOID).

[0079] Results: Sensitive to vancomycin, linezolid, chloramphenicol, cefepime clindamycin, see Table 8.

[0080] Table 8. Results of drug susceptibility test of bacterial strains.

[0081] drug sensitivity DA clindamycin 2ug sensitive E erythromycin 15ug intermediary LZD Linezolid 30ug sensitive C Chloramphenicol 30ug sensitive CTX Cefotaxime 30ug drug resistance P Penicillin 10ug —— VA vancomycin sensitive AMP Ampicillin 10ug drug resistance CRO Ceftriaxone 30ug drug resistanc...

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Abstract

The invention relates to the technical field of microorganisms, in particular to streptococcus symcsis 3-17 streptococcus and an application thereof. Experiment proves that the streptococcus providedby the invention is named as streptococcus symcsis sp.nov.3-17, and is registered and preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC 60684; and the streptococcus has broad spectrum resistance and long-term antagonizing stability, and can resist one or more of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, proteus vulgaris, enterobacter cloacae and acinetobacter baumannii. The streptococcus GDMCC 6068 provided by the invention can be applied as probiotics, corresponding products capable of being practically applied can be prepared from the streptococcus GDMCC 6068, and the streptococcus GDMCC 6068 has quite application tremendous potential in the field of medicine and pharmacy.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Streptococcus symcsis 3-17 streptococcus and application thereof. Background technique [0002] A probiotic is a kind of bacteria that itself or its metabolites can change the composition of the flora in a certain part of the host, regulate the balance of the microecological flora in the intestinal tract, promote the growth of beneficial bacteria, effectively inhibit the growth and reproduction of pathogenic bacteria, and regulate the immune function of the host mucosa and system. A class of living microorganisms that are beneficial to the host. Has other advantages. The phenomenon of multi-drug-resistant bacteria caused by the abuse of antibiotics has limited the actual use of antibiotics and the scope. In contrast, probiotics have the advantages of non-toxic, high-efficiency, no residue, and no drug resistance. [0003] Existing probiotic products are mainly aimed at...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/744A61P31/04C11D3/38C11D3/48C12R1/46
CPCA61K35/744A61P31/04C11D3/381C11D3/48C12R2001/46C12N1/205Y02A50/30
Inventor 肖纯凌齐贺李新鸣孙冶李舒音
Owner SHENYANG MEDICAL COLLEGE
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