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Compound microbial agent and preparation method therefor and treatment method for high-salinity nitrogen-containing wastewater

A compound microbial agent and microbial technology, applied in the field of microbial engineering, can solve problems affecting microbial enzyme activity, sludge settling performance, microbial wall separation, etc., to solve difficult biochemical treatment, improve biochemical treatment effect, and accelerate biochemical treatment. The effect of decomposition

Pending Publication Date: 2020-05-08
山东海景天环保科技股份公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, too high salinity has a greater impact on conventional biochemical treatment processes, mainly as follows: 1) too high salinity increases the osmotic pressure in the water environment, resulting in the separation of microbial biomass walls; 2) too high salinity The concentration will affect the activity of biological enzymes secreted by microorganisms; 3) Excessive salt concentration will affect the sludge settling performance
[0004] Due to the existence of the above problems, the application effect of conventional biochemical treatment processes in the treatment of high-salt nitrogen-containing wastewater is poor

Method used

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  • Compound microbial agent and preparation method therefor and treatment method for high-salinity nitrogen-containing wastewater
  • Compound microbial agent and preparation method therefor and treatment method for high-salinity nitrogen-containing wastewater

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preparation example Construction

[0029] The present invention also provides a kind of preparation method of composite microbial bacterial agent, comprises the following steps:

[0030] a) respectively preparing Candida tropicalis bacterial liquid, Pichia pastoris bacterial liquid, Bacillus chestnut bacterium liquid, Bacillus cereus bacterial liquid, Nitrobacter bacterial liquid and Halomonas bacterial liquid;

[0031] b) Mix the 6 kinds of bacterial liquids prepared in step a), then add a filter aid, and centrifuge to obtain the bacterial sludge;

[0032] c) mixing the bacteria sludge, the microbial growth promoter solution and the adsorption carrier, and drying in the shade to obtain a composite microbial bacterial agent.

[0033] In the preparation method provided by the present invention, first prepare Candida tropicalis bacterium liquid, Pichia pastoris bacterium liquid, Bacillus chestnut bacterium liquid, Bacillus cereus bacterium liquid, Nitrobacter bacterium liquid and Halomonas bacterium respectively ...

Embodiment 1

[0045] Preparation of each microbial culture.

[0046] Except for Nitrobacter, which was prepared by acclimation culture, the other 5 microbial strains were obtained by the way of preparing seed liquid-fermentation.

[0047] (1) Preparation of Candida tropicalis liquid:

[0048] (1.1) Seed liquid preparation: move the Candida tropicalis strains preserved at 4°C to 25°C for 4h activation; then inoculate into 500mL seed medium, set the culture temperature at 24°C, and shaker speed at 200r / min. Cultivate for 16 hours to obtain seed solution.

[0049] In step (1.1), the composition of the seed medium is by weight (w / w): 2% glucose, 1% yeast powder, 2% peptone, and the balance is water; the sterilization conditions are 121°C, 0.15 Mpa sterilization 20min.

[0050] (1.2) Fermentation: Inoculate in the fermentation medium with an inoculum size of 1% to 5% by volume percentage, culture temperature is 28°C, shaking table speed is 120r / min, culture time is 16h, and the number of effe...

Embodiment 2

[0075] Preparation of compound microbial bacterial agent:

[0076] (1) The 6 kinds of bacterial liquids prepared in Example 1 are in accordance with the following: Candida tropicalis bacterial liquid: Pichia pastoris bacterial liquid: Bacillus chestnut bacterial liquid: Bacillus cereus bacterial liquid: Nitrobacter bacterial liquid: Halomonas Bacterial liquid=1:1:3:3:35:40 volume ratio is mixed, obtain mixed bacterial liquid; Then add diatomaceous earth to mixed bacterial liquid according to the mass / volume ratio of 5g / 100mL; Centrifuge at 4000r / min for 40min in a three-legged centrifuge, collect the sludge and weigh it, and detect that the moisture content is 25%.

[0077] (2) According to protease 2%, amylase 4%, ferrous sulfate 0.03%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 0.3%, manganese sulfate 0.02%, the rest is configured into a solution by mass ratio of water; then add it into the bacteria sludge prepared in step (1) and stirred evenly to obtain a bac...

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Abstract

The invention belongs to the field of microbial engineering, in particular to a compound microbial agent and a preparation method therefor and a treatment method for high-salinity nitrogen-containingwastewater. The compound microbial agent comprises mixed bacteria, a microbial growth promoter and an adsorption carrier; the mixed bacteria and the microbial growth promoter are loaded onto the adsorption carrier; and the mixed bacteria include monilia tropicalis, pichia pastoris, bacillus badius, bacillus cereus, nitrobacteriaceae and halomonas sp. The compound microbial agent can obviously theresistance of biology to salinity, can be used for improving the effect of biochemical treatment of the high-salinity nitrogen-containing wastewater, and has a very broad application prospect.

Description

technical field [0001] The invention belongs to the field of microbial engineering, and in particular relates to a composite microbial bacterial agent, a preparation method thereof, and a treatment method for high-salt nitrogen-containing wastewater. Background technique [0002] High-salt nitrogen-containing wastewater exists in large quantities in different industries, such as leather industry, pharmaceutical industry, pickling industry, and printing and dyeing industry. The existence of nitrogen pollution in water bodies will not only cause drinking water supply safety problems, but also cause a series of problems such as seasonal hypoxia and eutrophication of surface water bodies, so denitrification treatment must be carried out before discharge. [0003] At present, there are many treatment methods for high-salt and nitrogen-containing wastewater, and biochemical treatment has been widely concerned because of its economical, high-efficiency, and low environmental pollut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/02C02F3/34C12R1/74C12R1/84C12R1/07C12R1/085C12R1/01
CPCC12N1/20C12N1/16C02F3/02C02F3/34C02F3/347Y02W10/10
Inventor 燕锡尧张英张映李川川刘文玉吴书超秦军
Owner 山东海景天环保科技股份公司
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