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Bacillus velezensis D1 with function of efficiently degrading feed starches in aquaculture water body and application of bacillus velezensis D1

A Bacillus, aquaculture technology, applied in the field of microorganisms, can solve problems such as environmental pollution, large economic losses, and accumulation of residual bait

Pending Publication Date: 2020-06-05
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the expansion of aquaculture scale and the improvement of aquaculture density, problems such as disease outbreaks and environmental pollution have become increasingly serious, and huge economic losses have greatly hindered the development of aquaculture.
Nowadays, intensive aquaculture systems are becoming more and more popular. In order to pursue production and economic benefits, inexperienced farmers often overfeed feed. Amino acid balance in the feed and other methods are used to increase the feed intake of farmed animals, but the intestinal tract of farmed animals is short, the residence time of the feed in the digestive tract is very limited, and the digestion efficiency is low, and most of the feed is completely excreted without digestion In vitro, it leads to the accumulation of a large number of residual baits, serious endogenous pollution in ponds, and large-scale aquaculture wastewater discharge pollution has always been a negative factor restricting the development of environmentally friendly

Method used

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  • Bacillus velezensis D1 with function of efficiently degrading feed starches in aquaculture water body and application of bacillus velezensis D1
  • Bacillus velezensis D1 with function of efficiently degrading feed starches in aquaculture water body and application of bacillus velezensis D1
  • Bacillus velezensis D1 with function of efficiently degrading feed starches in aquaculture water body and application of bacillus velezensis D1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Enrichment and Screening of Bacillus Velez D1 That Can Extracellularly Secrete Amylase

[0023] Sample source: Litopenaeus vannamei aquaculture water body in intelligent mariculture room of South China Sea Institute of Oceanology, Chinese Academy of Sciences.

[0024] The specific implementation steps are as follows: select healthy prawns with large vitality and full digestive tract in the prawn breeding pond, and bring them back to the laboratory immediately. The remaining seawater on the surface of the prawns was absorbed by filter paper, the body surface was wiped with 75% v / v alcohol for disinfection, dissected under aseptic conditions, and the complete intestinal tract was taken out. Rinse the intestinal tissue with sterile PBS (pH=7.4) buffer solution for 3 to 4 times, then put it into a 1.5mL sterile EP tube, add 1mL of PBS buffer solution to the sterile EP tube, and grind it evenly with a sterile grinding rod. Bacterial suspension was prepared by 10-f...

Embodiment 2

[0026] The identification of embodiment 2 Veles bacillus D1 bacterial classification

[0027] 1. Morphological observation

[0028] The purely cultured D1 strain was inoculated on the surface of 2216E solid medium by streaking method, and cultured in a 30°C incubator for 24 hours to observe the morphological characteristics of the colony. The bacterial colony is small and grows slowly on 2216E solid medium. The colony is dot-shaped, white and opaque, with rough edges, and the colony is relatively dry.

[0029] The 2216E solid medium is prepared by adding agar powder with a mass fraction of 1.5% to the 2216E liquid medium components, mixing and sterilizing. The 2216E liquid medium contains 5g of peptone per liter, 1g of yeast extract powder, 0.1g of ferric citrate, 19.45g of sodium chloride, 5.98g of magnesium chloride, 3.24g of sodium sulfate, 1.8g of calcium chloride, and 0.55g of potassium chloride , sodium carbonate 0.16g, potassium bromide 0.08g, strontium chloride 0.034...

Embodiment 3

[0040] Example 3 The Exploration of the Optimum Growth Conditions of Bacillus Velez D1

[0041] The effects of different pH, temperature and NaCl mass fraction (salinity) on the growth of Bacillus Velez D1 were studied respectively. Among them, the different temperatures are: 20°C, 25°C, 30°C, 37°C, 43°C; the different pH values ​​are: 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0; the different NaCl mass fractions are: 0 %, 1%, 2%, 3%, 4%, 5%.

[0042] In the above experiments, except for the investigation factors, the fermentation conditions were to inoculate the Bacillus veleus D1 culture solution in the LB fermentation medium with an inoculation amount of 1% (v / v), cultivate it at 30°C and 200rpm for 24h, and then take a sample Determination of OD for each condition 600 Value, the LB fermentation medium without inoculation was used as blank control. Take culture temperature, pH value, NaCl mass fraction as abscissa, OD 600 Values ​​plot the respective curves on the ordinate. see...

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Abstract

The invention discloses bacillus velezensis D1 with a function of efficiently degrading feed starches in an aquaculture water body and application of the bacillus velezensis D1. The preservation number of the bacillus velezensis D1 is GDMCC No:60943. The bacillus velezensis D1 disclosed by the invention is separated from intestinal tracts of prawns selves, and verification shows that the bacteriumis capable of extracellularly secreting amylase, and is capable of promoting digestion and absorption of starches and polysaccharides in feeds by bred animals after being immobilized in intestinal tracks of the bred animals. Gnotobiotic experiments of the bacterium with artemia show that the strain is free of stress or toxic effect on the bred animals even in a high concentration. The bacillus velezensis D1 grows optimally when the temperature is 25 DEG C, the primary pH value is 5.0 and the salinity is 2%; after the bacillus velezensis D1 is inoculated in a breeding water body, water qualitycan be stable, breeding targets grow healthily, and the bacterium is good in adaptability, high in security and environmentally friendly, can be used as a novel micro-ecological preparation for developing sustainable seawater breeding modes of green breeding, and has good application prospects.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of Bacillus Velez D1 capable of efficiently degrading feed starch in aquaculture water and an application thereof. Background technique [0002] As a popular seafood, Litopenaeus vannamei has extremely high nutritional value; China's shrimp farming industry has a huge scale, which has a major impact on the economic development of a region and the promotion of other industries. At present, my country is one of the world's important producers and exporters of aquatic products, and the output of prawns is still increasing year by year. According to the data of "China Fishery Statistical Yearbook 2019", the output of mariculture of prawns in China reached 55,800 tons in 2018. With the expansion of aquaculture scale and the improvement of aquaculture density, problems such as disease outbreaks and environmental pollution have become increasingly serious, resulting in huge ec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/80C12R1/07
CPCA23K10/18A23K50/80C12N1/205C12R2001/07
Inventor 陈偿丁雄祺谢媚杨艺滢方建相管云雁云龙
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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